36
V. SIMPULAN DAN SARAN
5.1 SIMPULAN
Hasil uji aplikasi BAL keempat formula yogurt menunjukkan bahwa yogurt F3 yang terdiri dari L. bulgaricus, S. thermophilus, dan L. fermentum memperlihatkan penampakan yang terbaik serta
didukung dengan nilai pH yang mendekati pH yogurt komersial. Dengan demikian, formula yogurt sinbiotik F3 merupakan formula terbaik yang berpotensi untuk dikembangkan menjadi produk pangan
alternatif untuk menjaga kesehatan saluran pencernaan. Uji in vivo pada tikus memperlihatkan bahwa pemberian yogurt sinbiotik F3 mampu mencegah diare. Hal ini diperlihatkan dari analisis kadar air
feses menunjukkan bahwa yogurt sinbiotik F3 mampu mempertahankan kondisi tubuh agar tetap sehat yang ditunjukkan dengan nilai kadar air feses tikus kelompok yogurt sinbiotik yang tidak berbeda
nyata dengan kadar air feses tikus kelompok kontrol negatif . Uji Imunomodulator pada organ tikus percobaan memperlihatkan bahwa yogurt sinbiotik
F3 mampu meningkatkan respon imun yang ditunjukkan dengan peningkatan jumlah limfosit limpa pada hari ke-14. Yogurt sinbiotik juga mampu menurunkan tingkat stress oksidatif pada organ hati
yang ditunjukkan dari konsentrasi MDA hati yang rendah pada hari ke-14 dan hari ke-21 dan aktivitas SOD hati dan ginjal yang lebih tinggi dibanding tikus yang diberikan EPEC pada hari ke-
14. Namun yogurt sinbiotik terpilih belum menunjukkan efek yang nyata pada kadar MDA ginjal.
5.2 SARAN
Kemampuan produk yogurt sinbiotik dalam melawan radikal bebas perlu diuji secara in vitro misalnya kapasitas antioksidan dengan uji DPPH untuk memperkuat hasil uji in vivo.
37
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1
LAMPIRAN
41
Lampiran 1. Pereaksi-pereaksi untuk Analisis MDA dan Kurva Standar TEP 1.1. TCA 15 wv
Sebanyak 25 g TCA dilarutkan dengan air bebas ion sampai volume 100 ml.
1.2. TBA Thiobarbaturic
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