Saran: SIMPULAN DAN SARAN

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Pp 505 LAMPIRAN Lampiran 1 : Tabel data skoring inisiasi pembungaan mindi di lokasi Gambung- Bandung No pohon Juli akhir awal agustus September Oktober meristem vegetatif apikal reproduktif meristem vegetatif apikal reproduktif meristem vegetatif apikal reproduktif 1 1 1 1 2 1 1 1 3 1 1 1 4 1 1 1 5 1 1 1 6 1 1 1 7 1 1 1 8 1 1 1 9 1 1 1 10 1 1 1 Total 7 3 3 7 4 6 Persenta se 70 30 30 70 40 60 Lampiran 2. Reseptivitas stigma dan Viabilitas Polen Tabel 1. Data skoring pengukuran reseptivitas stigma mindi Waktu pengambilan stigma Ulangan 1 2 3 Pk. 08.00 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Persentase 70 60 60 Pk.09.00 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Persentase 70 80 70 Pk. 10.00 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 1 Persentase 60 70 70 Pk.11.00 1 1 1 1 1 1 1 1 1 1 Lanjutan Tabel 1 Ulangan Waktu pengambilan stigma 1 2 3 1 1 1 1 1 1 1 1 1 1 Persentase 60 60 80 Pk.12.00 1 1 1 1 1 1 1 1 1 1 1 1 Persentase 40 40 40 Tabel 2. Analisis keragaman ANOVA pengaruh waktu pengambilan terhadap reseptivitas stigma df Sum of Squares Mean Square F Sig. Perlakuan 4 1973,3 493,3 10,57 0,001 Galat 10 466,7 46,7 Total 14 2440,0 Tabel 3 Uji beda rata-rata persentase reseptivitas stigma Perlakuan Nilai rata-rata Pk 08.00 63,33 A Pk 09.00 73,33 A Pk 10.00 66,67 A Pk 11.00 66,67 A Pk 12.00 40 B Keteranangan: Nilai yang diikuti huruf yang sama menyatakan tidak berbeda nyata pada taraf 5 Tabel 3. Data perkecambahan polen Ulangan Waktu pengambilan polen Pk. 07.00 Pk. 08.00 Pk. 09.00 Pk. 10.00 Pk. 11.00 1 3,13 0,00 0,00 3,23 0,00 2 1,52 0,84 6,25 4,44 2,44 3 0,00 12,50 16,39 14,29 0,00 4 4,69 2,56 13,04 16,67 4,55 5 11,29 4,55 10,00 11,11 9,68 6 15,13 0,00 0,00 0,00 8,33 7 9,09 8,43 0,00 0,00 19,05 8 14,29 16,00 0,00 0,00 0,00 9 4,17 0,00 0,00 0,00 0,00 Tabel 4. Analisis keragaman ANOVA pengaruh waktu pengambilan terhadap daya kecambah polen df Sum of Squares Mean Square F Sig. Perlakuan 4 2,08 0,52 0,29 0,880 Galat 40 70,53 1,76 Total 44 72,61 Tabel 5 Suhu rata-rata pada saat pengujian reseptivitas stigma dan viabilitas polen No Waktu Suhu rata-rata O C pengujian reseptivitas stigma Suhu rata-rata O C pengujian viabilitas polen 1 Pk.07.00-08.00 23,1 22,7 2 Pk.08.00-09.00 24,3 24,5 3 Pk.09.00-10.00 26,3 28,1 4 Pk.10.00-11.00 30,5 30,6 5 Pk.11.00-12.00 32,5 33,1 Lampiran 3. Penyerbukan mindi di Megamendung-Bogor Tabel 1. Data persentase bunga menjadi buah fruit set hasil penyerbukan tanaman mindi Ulangan Penyerbukan Alami kondisi terBuka PAB Penyerbukan Alami kondisi terTutup PAT Penyerbukan Buatan Polen dari pohon Sama PBPS Penyerbukan Buatan Polen dari pohon Lain PBPL 1 0,77 0,71 0,50 0,50 2 0,79 0,27 0,50 0,80 3 0,75 0,72 0,25 0,80 Rataan 77 57 42 70 A B Gambar 1 A. Proses emaskulasi pemotongan bagian petal pada penyerbukan buatan B. Proses penutupan malai bunga setelah penyerbukan buatan danatau alami PAT, PBPS dan PBPL Lampiran 4 : Produksi buah mindi di lima lokasi di Jawa Barat Lokasi Rata-rata produksi buah kgphn Tahun 2009 Tahun 2010 Rata-rata per tahun Ds. Legok Huni, Kab. Purwakarta 14,7±2,1 5,0±0,7 9,9±1,3 Ds. Mekarsari Gambung, Kab. Bandung 5,1±0,6 1,2±0,1 3,1±0,4 Ds. Babakan Rema, Kab. Kuningan 5,8±1,5 5,6±1,5 5,7±1,1 Ds. Nagrak, Kab. Bogor 4,3±0,5 5,5±0,7 4,9±0,4 Ds. Sukakarya, Kab. Bogor 5,1±0,3 5,2±0,3 5,1±0,2 Sumber: Atmandhini 2011 Lampiran 5: Data Pengukuran Karakter Fenotipik Pohon Induk Mindi Tabel 1. Data rata-rata perkecambahan mindi dari lokasi Wanayasa Purwakarta Tanggal penaburan: 09062011 Praperlakuan: H2SO4 45 Jumlah benih: 4 x 25 benih No phn ind Daya Berkecambah DB Waktu mulai berkecambah WMB hari Total hari berkecambah TBhari Rata-rata harian berkecambahRH B hr 2 21 45,25 58,00 0,33 8 10 41,00 51,00 0,20 13 4 39,00 46,50 0,44 15 41 24,50 57,50 0,68 17 12 41,00 49,50 0,24 24 16 24,25 54,75 0,30 28 22 39,50 65,00 0,33 29 34 23,75 53,25 0,63 20 24 41,50 62,00 0,39 Tabel 2. Data rata-rata dari 20 benih untuk pengukuran struktur benih mindi No phn Variabel Panjang mm Diameter mm Berat g 2 12,426 ±1,802 7,941±0,625 0,410±0,113 8 11,634±1,594 7,961±0,807 0,360±0,102 13 12,674±1,635 8,411± 0,769 0,466±0,112 15 11,754±1,326 8,198±0,760 0,370±0,086 17 11,446±1,304 7,759±0,807 0,333±0,110 24 12,558±1,882 8,103±0,542 0,403±0,103 28 11,623±1,389 7,662±0,694 0,327±0,090 29 12,430±1,501 8,388±0,724 0,432±0,113 20 12,579±1,519 8,099±0,657 0,398±0,104 Tabel 3 Data rata-rata dari 10 tanaman untuk pengukuran morfologi semai mindi umur 6 minggu Tinggi total batang semai TTBS cm Tinggi batang bebas daun TBDS cm Diame ter batang semai DBS cm Kekoko han batang semai RTDS indeks Kelurus an batang semai KLBS indeks Panjan g pucuk PPS cm Panjan g daun terpanj ang PDTS cm Jumla h daun JDS helai Panjan g anak daun PADS cm Lebar anak daun LAD S cm Jarak anak daun perta ma JAD P cm Rasio panjan g lebar anak daunR PLDS cm Panja ng petiol PPD S cm Jarak helai daun terleb ar JLD S cm Bentuk lamina daun BLDS indeks Rasio panjang petiol terhadap panjang daun RPDS indeks Rasio lebar lamina thdp panjang daun LWR indeks Ph2 14,12 8,26 1,497 9,723 2,9 5,86 10,18 5,9 3,99 1,84 2,22 2,199 0,54 1,56 0,395 0,135 22,89 Ph13 17,98 6,05 1,875 9,686 2,6 11,93 12,19 11 4,59 1,99 1,18 2,309 0,51 1,74 0,385 0,111 21,77 Ph8 13,94 6,14 1,908 7,654 2,8 7,8 12,29 10 4,84 2,43 0,98 2,094 0,82 1,89 0,380 0,17 24,57 Ph15 13,27 6,02 1,307 10,573 2,7 7,15 9,99 8,8 3,53 1,82 1,24 1,939 5,2 1,67 0,495 1,468 26,54 Ph17 14,71 5,49 1,518 9,859 2,9 9,22 11,35 9,1 4,68 2,16 1,3 2,163 0,68 1,83 0,389 0,145 23,32 Ph24 17,00 3,98 6,182 4,564 2,8 13,02 15,56 9 5,42 2,52 1,97 2,152 0,72 1,83 0,342 0,129 39,03 Ph28 11,66 3,53 4,327 5,007 2,9 8,121 9,76 8,1 3,98 1,94 1,45 2,063 0,54 1,56 0,398 0,139 24,62 Ph29 15,92 5,28 2,024 8,043 2,8 10,64 13,04 10,7 4,94 2,33 1,06 2,103 0,7 1,85 0,372 0,14 23,72 Ph20 11,26 3,81 2,563 5,602 2,9 7,45 9,67 8,7 3,69 1,7 1,26 2,235 0,47 1,39 0,388 0,135 22,77 122 Lampiran 6: Prosedur Laboratorium Analisis DNA dengan Penanda Mikrosatelit

I. PERALATAN DAN BAHAN 1.1. Peralatan:

alat-alat gelas gelas ukur, erlenmeyer, mikropipet, tube, mortar dan pestel, rak tube dll, Freezer, inkubator, sentrifuge, neraca analitik, stirer, desicator, hotplate,kamera digital, UV transluminator, vortex and peltier thermal cycler alat untuk PCR, microwave, electrophorecys chamber vertikal,

1.2. Bahan :

material daun muda, bahan-bahan kimia

II. Persiapan larutan : 1. Larutan Tris-HCl 1M

Digunakan untuk tambahan dalam pembuatan buffer ekstrak. Pembuatan: timbang Tris-HCl sebanyak 1,817 g + akuades 15 ml, larutkan kemudian diukur pHnya 8,0.

2. Larutan NaCl 5M

Digunakan untuk memekatkan, memisahkan DNA dari larutan dan mengendapkan DNA serta membentuk benang-benang nukleat, Endapan DNA akan tampak seperti tepung berwarna putih. Pembuatan: timbang NaCl sebanyak 4,383 g + akuades 15 ml, larutkan sampai homogen.

3. Larutan EDTAEtilen Diamin Tetra Acetat 0,5M

Larutan ini merupakan salah satu komponen untuk buffer ekstraksi yang berfungsi sebagai pengikat ion bivalen Mg 2- dan Ca 2- yang menjadi kofaktor bagi enzim nuklease. Adanya EDTA menyebabkan enzim nuklease menjadi tidak aktif. Pembuatan: Timbang EDTA sebanyak 2,79 g + akuades 15 ml dilarutkan sampai homogen.

4. CTABCetil Trimetil Amonium Bromida 10 wv

Digunakan untuk melisis dinding sel tanaman sehingga isi sel bisa keluar dengan merusak protein dan melepaskan sebagian polisakarida dari DNA. Pembuatan : Timbang CTAB sebanyak 1,5 g + akuades 15 ml, larutkan sampai homogen.

5. Mercaptoetanol 1 vv

Digunakan untuk meisolasi DNA dan dapat mereduksi ikatan sulfida dari enzim polifenol oksidase. Pembuatan: larutkan 1 ml mercatoetanol dalam 100 ml akuades sampai homogen.

6. PVP 1

Digunakan untuk menghambat enzim polifenoloksidase dan mendegradasi rantai DNA Pembuatan: Timbang PVP 1 gr + 100 ml akuades larutka sampai homogen

7. Chloroform : Isoamylalkohol 24:1

Khloroform digunakan untuk membersihkan DNA dari protein dan polisakarida,sedangkan isoamylalkohol berfungsi untuk mengurangi busa khloroform yang terjadi pada saat pengocokan. Pembuatan: Campurkan khloroform sebanyak 24 ml dengan 1 ml isoamylalkohol, aduk sampai homogen.

8. Propanol dingin

Digunakan untuk mengendapkan DNA. Pebuatan: dinginkan propanol murni 25 ml dalam freezer untuk pengendapan DNA