54
2 0.0135 0.0128 0.0140 5.469 9.375
3 0.0087 0.0086 0.0095 1.163 10.465
4 0.0240 0.0232 0.0238 3.448 2.586
5 0.0226 0.0212 0.0222 6.604 4.717
P2 1 0.0218 0.0212 0.0234 2.830 5.166 2.968 57.45
10.377 8.980 3.608 40.17
2 0.0260 0.0257 0.0285 1.167 10.895
3 0.0172 0.0161 0.0179 6.832 11.180
4 0.0207 0.0193 0.0198 7.254 2.591
5 0.0153 0.0142 0.0156 7.746 9.859
P3 1 0.0251 0.0239 0.0262 5.021 2.220 1.719 77.41
9.623 9.352 0.459
4.90 2 0.0266 0.0265 0.0291 0.377
9.811 3 0.0161 0.0158 0.0173 1.899
9.494 4 0.0265 0.0261 0.0285 1.533
9.195 5 0.0225 0.0220 0.0239 2.273
8.636
7. Data Uji Ketahanan Pelipatan
Frekuensi
Basis 1 24
Basis 2 45
Basis 3 44
PVP 1 77
PVP 2 50
PVP 3 25
55
Lampiran 7. Data Perhitungan Dosis dan Kandungan Obat Hydrocolloid Matrix Piroksikam
1. Data Perhitungan Dosis Piroksikam dalam Setiap Sediaan Siap Pakai
Diameter sediaan = 1 cm Diameter petri
= 9.5 cm Luas lingkaran sediaan =
� =
� .7 = 70.911 cm
2
Luas lingkaran petri =
� =
� . = 0.786 cm
2
Dosis obat yang dimasukkan dalam setiap formula: P1 = 184.70 mg
P2 = 182.70 mg P3 = 182.00 mg
Dari setiap formula dapat dibuat menjadi 3 petri, maka dosis setiap petri:
P1 =
. �
= 61.657 mg P2 =
. �
= 60.900 mg P3 =
. �
= 60.667 mg
Perbandingan:
� � � � � � � �
� � � � � � �
� =
� ���
� � � � � � �
��� P1
=
. ��
. ��
� . �� = 0.682179 mg = 682.179 �g
P2 =
. ��
. ��
� . �� = 0.674792 mg = 674.792 �g
P3 =
. ��
. ��
� . �� = 0.672207 mg = 672.207 �g
2. Data Perhitungan Larutan Baku Piroksikam
C =
mg �
= 0,2
mg mL
= 200 �gmL
3. Data Perhitungan Larutan Intermediet Piroksikam
10 mL x 0,2 mgmL = C
2
x 100 mL = 0.02
mg mL
= 20 �gmL
4. Konsentrasi Larutan Seri Piroksikam
Seri 1 1.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 2 �gmL
Seri 2 2.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 4 �gmL
Seri 3 3.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 6 �gmL
56 Seri 4
4.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 8 �gmL
Seri 5 5.0 mL x 0.02 mgmL = C
1
x 10 mL C
= 10 �gmL
Seri 6 6.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 12 �gmL
Seri 7 7.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 14 �gmL
Seri 8 8.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 16 �gmL
Seri 9 9.0 mL x 0.02 mgmL = C
1
x 10 mL C
1
= 18 �gmL
Seri 10 10.0 mL x 0.2 mgmL = C
1
x 100 mL C
1
= 20 �gmL
5. Kurva Kalibrasi Piroksikam
Konsentrasi Abs
�̅ Abs SD
CV µgml
1 2
3
0.2 0.010
0.008 0.009
0.0090 0.0010
11.111 0.4
0.018 0.021
0.016 0.0183
0.0025 13.727
0.6 0.035
0.034 0.033
0.0340 0.0010
2.941 0.8
0.040 0.039
0.039 0.0393
0.0006 1.468
1 0.045
0.046 0.045
0.0453 0.0006
1.274 1.2
0.058 0.058
0.059 0.0583
0.0006 0.990
1.4 0.066
0.067 0.067
0.0667 0.0006
0.866 1.6
0.075 0.076
0.076 0.0757
0.0006 0.763
1.8 0.084
0.083 0.084
0.0837 0.0006
0.690 2
0.101 0.099
0.099 0.0997
0.0012 1.159
4 0.198
0.197 0.188
0.1943 0.0055
2.834 6
0.297 0.296
0.290 0.2943
0.0038 1.286
8 0.387
0.391 0.385
0.3877 0.0031
0.788 10
0.488 0.491
0.475 0.4847
0.0085 1.755
12 0.582
0.588 0.590
0.5867 0.0042
0.710 14
0.682 0.686
0.673 0.6803
0.0067 0.979
16 0.778
0.784 0.774
0.7787 0.0050
0.646 18
0.867 0.867
0.860 0.8647
0.0040 0.467
20 0.963
0.963 0.944
0.9567 0.0110
1.147
57 Gambar 1. Kurva Kalibrasi Piroksikam
6. Linearitas dan Sensitivitas Metode Parameter
