78
PVP 3 15
vii. Uji Kandungan Obat dalam Sediaan
Formula Abs
Kadar Terukur
µgmL Q Obat
µg Q
Obat mg
�̅ Q Obat
SD CV
Q Obat Teoritis
mg Recovery
P1 1 0.570
11.807 590.353 0.590 0.571 0.056
9.888 0.682
83.763 2 0.484
10.023 501.141 0.501 3 0.560
11.600 579.979 0.580 4 0.517
10.707 535.373 0.535 5 0.627
12.990 649.481 0.649
P2 1 0.677
14.027 701.349 0.701 0.655 0.088 13.393
0.675 97.080
2 0.556 11.517 575.830 0.576
3 0.545 11.288 564.419 0.564
4 0.747 15.479 773.963 0.774
5 0.638 13.218 660.892 0.661
P3 1 0.509
10.541 527.075 0.527 0.533 0.034
6.394 0.672
79.298 2 0.551
11.413 570.643 0.571 3 0.493
10.210 510.477 0.510 4 0.545
11.288 564.419 0.564 5 0.475
9.836 491.805 0.492
h. Stabilitas Minggu IV Suhu 45
o
C i. Uji Organoleptis
Formula Warna
Kejernihan Kehalusan
Kelengketan
PVP 1 Kuning
Terdispersi Halus
Kering PVP 2
Kuning Terdispersi
Halus Kering
PVP 3 Kuning
Terdispersi Agak kasar ada
gelembung Kering
ii. Uji Keseragaman Bobot
PVP 1 PVP 2
PVP 3
1 0.0130
0.0108 0.0099
2 0.0076
0.0127 0.0120
3 0.0113
0.0109 0.0157
4 0.0121
0.0118 0.0172
5 0.0107
0.0136 0.0151
6 0.0087
0.0145 0.0087
7 0.0055
0.0168 0.0160
8 0.0144
0.0172 0.0158
9 0.0111
0.0107 0.0081
10 0.0134
0.0126 0.0068
79
�̅
0.01078 0.01316
0.01253
SD 0.0026332
0.0022509 0.0036813
CV 24.43
17.10 29.38
iii. Uji Ketebalan
PVP 1 PVP 2
PVP 3
1 0.15
0.15 0.20
2 0.15
0.15 0.20
3 0.15
0.15 0.20
4 0.15
0.15 0.20
5 0.15
0.15 0.20
�̅ 0.15
0.15 0.20
iv. Uji pH Sediaan
pH
PVP 1 7.2
PVP 2 7.2
PVP 3 7
v. Uji Moisture Content dan Moisture Absorption
Bobot awal
Bobot akhir
Bobot akhir
MC �̅
MC SD
CV MA
�̅ MA
SD CV
P1 1 0.0130 0.0128 0.0133 1.562
1.010 1.084 107.339 3.906
3.572 1.934 54.155 2 0.0076 0.0076
0.0078 0.000 2.632
3 0.0113 0.0113 0.0116 0.000
2.655 4 0.0121 0.0118
0.0126 2.542 6.780
5 0.0107 0.0106 0.0108 0.943
1.887 P2 1 0.0108 0.0107
0.0109 0.935 0.983 1.013 103.055
1.869 4.850 3.037 62.624
2 0.0127 0.0127 0.0138 0.000
8.661 3 0.0109 0.0109
0.0112 0.000 2.752
4 0.0118 0.0116 0.012 1.724
3.448 5 0.0136 0.0133
0.0143 2.256 7.519
P3 1 0.0099 0.0099 0.0106 0.000
0.746 0.787 105.378 7.071
3.806 2.614 68.675 2 0.0120 0.0120
0.0126 0.000 5.000
3 0.0157 0.0155 0.0155 1.290
0.000 4 0.0172 0.0169
0.0174 1.775 2.959
5 0.0151 0.0150 0.0156 0.667
4.000
80
vi. Uji Ketahanan Pelipatan
Frekuensi
PVP 1 25
PVP 2 40
PVP 3 15
vii. Uji Kandungan Obat dalam Sediaan
Formula Abs
Kadar Terukur
µgmL Q Obat
µg Q
Obat mg
�̅ Q Obat
SD CV
Q Obat Teoritis
mg Recovery
P1 1 0.515
10.666 533.299 0.533 0.477 0.085 17.781
0.682 70.013
2 0.566 11.724 586.203 0.586
3 0.463 9.587 479.357 0.479
4 0.386 7.990 399.481 0.399
5 0.376 7.782 389.108 0.389
P2 1 0.648
13.425 671.266 0.671 0.651 0.045
6.883 0.675
96.496 2 0.552
11.434 571.680 0.572 3 0.641
13.280 664.004 0.664 4 0.655
13.571 678.527 0.679 5 0.648
13.425 671.266 0.671
P3 1 0.541
11.205 560.270 0.560 0.531 0.034
6.365 0.672
79.082 2 0.508
10.521 526.037 0.526 3 0.551
11.413 570.643 0.571 4 0.493
10.210 510.477 0.510 5 0.473
9.795 489.730 0.490
81
Lampiran 10. Data Uji Iritasi Hydrocolloid Matrix Piroksikam 1. Data Uji Iritasi Kulit
Kontrol Positif Etil Asetat + Hypafix Kelinci
24 jam 48 jam
72 jam Eritema
Udem Eritema
Udem Eritema
Udem 1
2 2
2 2
3 3
3 3
2 2
2 Total
7 7
7 Indeks Iritasi Primer : 7.0
Kesimpulan : Iritan kuat Kontrol Negatif Kasa + Hypafix
Kelinci 24 jam
48 jam 72 jam
Eritema Udem
Eritema Udem
Eritema Udem
1 2
3 Total
Indeks Iritasi Primer : 0.0 Kesimpulan : Sangat ringan
Basis 1 Kelinci
24 jam 48 jam
72 jam Eritema
Udem Eritema
Udem Eritema
Udem 1
2 3
Total Indeks Iritasi Primer : 0.0
Kesimpulan : Sangat ringan Basis 2
Kelinci 24 jam
48 jam 72 jam
Eritema Udem
Eritema Udem
Eritema Udem
1 2
3 Total
Indeks Iritasi Primer : 0.0 Kesimpulan : Sangat ringan
82
Basis 3 Kelinci
24 jam 48 jam
72 jam Eritema
Udem Eritema
Udem Eritema
Udem 1
2 3
Total Indeks Iritasi Primer : 0.0
Kesimpulan : Sangat ringan
83
Lampiran 11. Data Persen Wound Closure a. Tikus Diabetes
Hari ke-
Basis 2 Formula PVP 2
Kontrol Tikus 1 Tikus 2 Tikus 3 Tikus 1 Tikus 2 Tikus 3 Tikus 1 Tikus 2 Tikus 3
0.000 0.000
0.000 0.000
0.000 0.000
0.000 0.000
0.000
1 3.846
2.706 5.796
3.888 5.666
12.364 3.785
2.575 3.586
2
8.379 13.745
16.847 9.268
16.362 14.984
13.898 12.297
10.812
3 10.323
21.281 21.281
13.706 20.675
15.292 24.212
14.793 19.627
4
25.296 26.618
37.600 17.242
25.588 21.281
31.435 21.180
29.044
5 38.693
34.047 46.957
20.022 36.586
34.054 46.859
37.156 30.262
6 40.916
49.247 51.476
32.698 44.326
40.534 64.085
42.100 51.060
7 53.142
62.179 69.909
55.020 63.621
50.289 67.313
61.545 57.923
8
66.087 73.462
76.383 65.283
79.199 61.540
75.591 76.538
76.936
9 80.301
84.261 80.363
77.747 83.203
69.063 79.260
82.425 89.027
10
86.914 87.469
83.863 86.428
86.173 75.086
88.116 86.539
89.115
11 92.553
91.924 85.674
90.046 92.010
83.553 92.528
91.516 90.730
12 94.340
93.014 87.865
92.681 93.890
87.174 92.600
93.665 92.956
13 96.684
96.098 90.537
95.335 94.691
90.727 95.525
94.784 93.956
14
97.674 97.247
92.166 97.270
96.839 94.404
96.217 96.040
94.890
15 98.489
98.650 95.011
98.154 98.641
96.231 97.299
97.412 96.063
16 100.000 100.000
97.693 100.000 100.000 98.652 100.000
98.995 98.689
17 100.000
100.000 100.000 100.000
b. Tikus Non Diabetes
