Plant Materials Research Methods Observations Data Analysis

Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015 ISBN: 978-602-74204-0-3 Biology Page 430 Indonesian sorghum production is still very low, even in general sorghum products not yet available in the markets. Associated with energy, in some countries such as the USA, India and China, sorghum has been used as a raw material for making ethanol fuel bioethanol. Traditionally, ethanol has a longer produced from molasses sugar cane processing waste results sugarcane. Although the price is relatively cheaper sugar cane molasses, but sorghum bioethanol can compete given the multiple advantages compared to sugar cane sorghum crops are as follows 1 sorghum plants have seeds and biomass production are much higher than sugarcane; 2 Adaptation sorghum cane is much broader than that sorghum can be grown in almost any type of land, both arable land and marginal land; 3 have the properties of sorghum plants more resistant to drought, high salinity and waterlogging water lodging; 4 Sorghum requires relatively less fertilizer and maintenance easier than sugarcane 5 The rate of growth of the sorghum crop is much faster than the cane; 6 Planting of sorghum easier, the seed needs only 4.5-5 kg ha compared to the cane that require stem cuttings 4500-6000; 7 age faster sorghum harvest is only 4 months, compared to sugar cane harvested at 7 months; 8 Sorghum can produced so for once planting can be harvested several times. Sorghum can be grown on marginal land in between ground with a certain salinity levels. Some cultivars that have been tested as UPCA-S1, Saber, Rare Kito, UGM SS1, Durra, B-69, B-72, B-75, B-76, B-83, B-90, B-92, B- 95, and B-100. But not all cultivars of sorghum has been tested its response on land that has high salinity levels, so it is considered necessary to perform the test. This study aimed to test the tolerability sorghum saline soil conditions. 2. Materials and Methods 2.1. Place and Time Research The research was conducted in the greenhouse complex Laboratory Department of Biology, Faculty of Mathematics and Natural Sciences UNIMA for field research. Measurement of plant fresh weight, dry weight of plants, and dissolved solids performed at the Laboratory of Department of Biology, Faculty of Mathematics and Natural Science, Manado State University. The research was conducted in August 2014 until October 2014.

2.2. Plant Materials

Materials used are common salt NaCl and sorghum varieties Kawali, while the tool is used as a measuring cup, polybags, meter, and oven.

2.3. Research Methods

Trial is a single factor and three replications arranged in a completely randomized design CRD. Each treatment and replications of five examples of plants. The main factor is the concentration of salt solution. The concentration of the salt solution used is 0 mM control, 150 mM, 300 mM, and 450 mM. Treatment given salt concentration since the beginning of the planting is when the seeds sown by spraying saline into the nursery until the media field capacity, then the next watering with water wells. After the age of 14 HST, the plants were transferred to polybags that has been treated with a solution of salt 1 liter. Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015 ISBN: 978-602-74204-0-3 Biology Page 431

2.4. Observations

Observations were made on vegetative growth to see the morphology and physiology of sorghum. Vegetative growth components include: 1 plant height cm, the plant height is measured from the neck to the tip of the leaf crops are aligned parallel to the rod and measured the vegetative phase; 2 stem diameter cm, measured at the root of the neck that is the boundary between the stem and roots; 3 the number of leaves pieces, all the leaves are formed.

2.5. Data Analysis

Data were analyzed by analysis of variance using SAS 9.1 for Windows program and proceed with the testing using the Least Significant Difference Test LSDT.

3. Results and Discussion