Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
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tannins and steroids. The chromatography analysis showed that avocado leaf extract, selada darat and gedi contained polyphenols while the results based on a paper chromatography Rf
value compared to the references revealed the existence of flavonoids, a group of flavonols: mirisetin, Quercetin and keemferol as well as biflavonil group: kayaflavon. The toxicity test
showed LC
50
250 ppm to be relatively safe for consumption.
4.2. Suggestion
Further investigation on the exact active compounds in the plant extracts both tested
in vivo
and
in vitro
would be necessary in order to develop phytopharmacological antihiperlipidemic medicinal plants native to South Minahasa.
Acknowledgement
The authors thanked to DP2M Dikti through the Research Department of the State University of Manado for the financial support by the National Strategic Grants Batch I 2009.
References
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ISBN: 978-602-74204-0-3
Biology Page 394
Kramer FB, and Sheen WJ, 2006. Hormone-sensitive Lipase Knockouts. Review of Nutrition and Metabolism.
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http:www.scientificpsychic.comsitemap.html
Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 395
IMPACT OF BOTANICAL INSECTICIDES DERIVED FROM
Pangium edule
Reinw., AND
Annona muricata
L. EXTRACTS ON THE DIAMONDBACK MOTH,
Plutella xylostella
L.
Ernest H. Sakul
1
1
Department of Biology, Faculty of Mathematics and Natural Sciences, Manado State University, Minahasa, Indonesia
ernesthannysakulgmail.com
Abstract Insecticidal properties of fractioned extracts from
Pangium edule
Reinw seed and
Annona muricata
seed against
Plutella xylostella
larvae were investigated in the laboratory. The study was initiated to investigate the possibility of using botanical pesticides to control
P.xylostella,
a serious cosmopolitan pest of crucifer plants. The study aims to determine the most effective concentration and the most
active extract; to evaluate the different extract concentrations on the treated larvae; and to characterize the phytochemical contents of the most effective extracts fraction.
The study was an experiment initiated by test of phytochemical screening test in order to discover the presence of secondary metabolites in the extracts. It was followed by the test of mortality of the
diamondback moth larvae. Furthermore, the extracts hexane fraction and etanol fraction, were conducted with completely randomized design; The LC
50
values were determined following probit analysis, the data were treated in the software programme IBM Statistic SPSS 20.
Results showed that n-hexane fraction is the most effective againts larvae LC
50-48h
= 12,71 mgL from
P.edule
seed extract, LC
50-48h
= 50,81 mgL from
A.muricata
seed extract. Larva mortality was highest using 1000 ppm n-hexane fraction 96,6 derived from
P.edule
seed extract, 93,3 derived from
A.muricata
seed extract. The ethanol fraction tested positive for alkaloid, saponins, flavonoids, terpenoids, phenol and tannins.
N-hexane fraction of
P.edule
seed extract
,
and
A.muricata
seed extract are an effective botanical insecticides exhibiting larvicidal and antifeedant properties against
P.xylostella
thus it can be alternative to synthethic insecticides. Results indicate that these botanical insecticides have good
possibilities for control of
P.
xylostella. Further work is necessary to evaluate and characterize the active components of the extract fractions and its efficacy in the field.
Keywords :
Botanical insecticides, Pangium edule, Annona muricata, Phytochemical screening, Plutella xylostella
.
1. Introduction