Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 508
concentration given.
B. asiatica
seed extract treatment can reduce the number of eggs ranges from 41.4 to 112.4 grains. The results of analysis of variance
: 005 the effect of B. asiatica extract to the fecundity of adult females showed significantly different results each at a
concentration of 0.30 to 0.45 and 0.15 _ 0:30. Results of analysis of variance showed that the effects of extracts of the old stadium and the
percentage of eggs hatching eggs on the 5th level tested concentrations significantly different concentrations of 0. Average -rata rama hatching egg stage on domesticum L- seed extract
treatment ranged from 4.52 to 4.79 days while the average percentage of eggs hatching on all treatments ranged between 93.50 - 97.09.
Treatment with B. asiatica extract can give real effect to the old stadium hatching eggs with an average ranging from 4.30 to 4.81 while the average percentage of procedures for the eggs
to hatch ranges between 88.66 - 99.07. Treatment with both types of these extracts resulted in a decrease teradinya fecundity of female imago
S. exigua
tested. This is in line with the theory put forward by Wigglesworth 1977 that the quality and the quantity of larval
food effect on fecundity imago, where fecundity of adult females of
S. exigua
decline may be due to the short life of the imago itself because of the treatment with
B. asiatica
seed extract. Other factors suspected cause of the decline in fecundity are the active ingredients contained
in these extracts can affect the system in the process pernbentukan reproductive organs and hormone function, especially hormones that play a role in the process vitelogenesis.
5. Conclusion and Suggestion 5.1. Conclusion
1. Extract the seeds of
B. asiatica
seeds were tested on larvae of
Spodoptera exigua
have insecticidal activity. The toxicity is expressed in terms of the highest LC
50
owned namely 0.21. 2.
Nature of
B. asiatica
antifeedant highest in LC
95
- LC
15
reached 34.01 whereas only 14.3 .
3. Extract the seeds of
B. asiatica
each sublethal concentrations can : a. Extracts inhibit larval development to the magnitude of the inhibitory reaching
1.92 to 2.45 days. b. Lowering the percentage of success of the pupa into the imago highest sex ratio
is 89.40 with the number of adult females more than males. c. Lowers fecundity of adult females with many eggs
S. exigua
relatively the same for the second extract ranged from 433.8 to 504, 80 grains.
d. Shortening the life long imago with higher concentration given the lower the longer life of the imago
S. exigua
. Effect of extracts of the long-life higher at 7.97 days.
5.2. Suggestions
Ethyl acetate extract fraction from seed seeds Bitung
B. asiatica
have good chances to be developed as an alternative pest control onion caterpillar
S. exigua
. For the purpose of development is required so it can be field testing the effectiveness against the target pest and
its effect on other non-target organisms. Further studies need to be performed to isolate and identify the active compounds from the extracts
B. asiatica.
Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 509
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Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 510
SECONDARY METABOLITES COMPOUNDS IN TOMBILI
Caesalpinia bonduc
L. SEEDS
Chairunnisah J. Lamangantjo
1
, Weny Musa
2
, Jusna Ahmad
1
, Suci Safitri Dg. Palalo
2
1
Department of Biology, Faculty of Ma thematics and Science, State University Gorontalo, Gorontalo City, Indonesia
2
Department of Chemistry, F aculty of Mathematics and Science, State University Gorontalo, Gorontalo City, Indonesia
chairunnisahjlgmail.com
Abstract This study aims to determine and identify secondary metabolites contained in the seeds Tombili
Caesalpinia bonduc
L.. Compounds isolated by maceration and fractionation, phytochemical test, separation and purification, and identified by UV-Vis spectroscopy and IR. Samples were macerated
with 1.5 kg of methanol and produce 85.79 grams of viscous extract, fractionation of 33.34 grams of viscous extract with n-hexane, ethyl acetate, and water solvent each fraction produce 4.54 gram, 1.6
grams, and 9.34 grams of viscous extract respectively. Result of phytochemical test of isolates showed positive contain against flavonoids, terpenoids, alkaloids, tannins and saponins. The results showed
that the content of the metabolites derived from the Tombili seeds be in the form of white crystals. Two-dimensional TLC test with two eluent mixture ratio that is n-hexane: ethyl acetate 9: 1 E
1
isolates with Rf 0.295 and n-hexane: MTC: acetone 7: 1: 2 isolates E
2
with Rf 0.55 produce a single spot.
Keywords: Secondary metabolites compounds,
Caesalpinia bonduc
L.
1. Introduction