Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 381
In the chromatogram image 16 S RNA isolates formed band at 1500 bp this shows that the primers used to amplify the gene can have 16 S RNA. Evident band formed thus the migration
process went well.Bacterial identification methods are widely recommended is the analysis of bacterial DNA through the reading of the nucleotide sequence of nitrogenous bases making up
the gene 16S rDNA fragments bacteria. Judging from several considerations, this method is considered better than the phenotypic analysis method. The first consideration is the 16S
rDNA gene of almost all species of bacteria have been determined sequence of nitrogenous bases that can be used as guidelines if found new species. The second consideration is the
base sequence of 16S rDNA gene nitrogen has a lower intraspecific diversity than other protein-coding genes, as well as the nature of 16S rDNA fragments were sustainable. To
obtain the base sequence or sequences should be in sequencing PCR products. Product sequencing will be used for the analysis of kinship phylogeny isolates obtained compared
with the existing data in the NCBI or other molecular data center.
4. Conclusion
From the results of this study concluded that: 1. Found 5 isolates of caterpillar pests in cabbage which shaped Cocus 4 isolates, 3 isolates of gram + and two isolates of gram -, all
isolates were able to degrade D-Glucose. Color white isolates seeds, white yellowish and brownish white. Generally isolates are not motile. Growth in the media to the surface. 2. The
results of DNA extraction and amplification of gene 16 S RNA, indicating the purity and concentration of DNA is good, confirmed by electrophoresis, amplicons formed a band at
1500 bp according to the characteristics of the 16 S RNA gene.
Acknowledgements
Presented thanks to the Research Institute of the State University of Manado which has funded this research through the application of science and technology research skim in 2014.
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PHYTOCHEMICALS, TOXICITY, AND ANTIHYPERLIPIDEMIC ACTIVITY OF SEVERAL MEDICINAL PLANTS FROM SOUTH MINAHASA
Rudi Alexander Repi
1,2
, Mokosuli Yermia Semuel
1,2
1
Department of Biology, Faculty of Mathematics and Natural Sciences, State University of Manado, Minahasa, Indonesia
2
Laboratorium of Bioactivity and Molecular Biology, Faculty of Mathematics and Natural Sciences, State University of Manado, Minahasa, Indonesia
labbiomolunima gmail.com
Abstract A research has been done to develop antihyperlipidemic phytopharmacological ethnomedicinal plants
from South Minahasa. The research aimed to know the combination effect of plant extracts to mice lipid profile. The research consisted of four steps, the first step was the identification of the plants, the
second was the extraction and phytochemicals analysis based on Harborne and Chromatography methods. The third was Brine Shrimp Lethality Tests BSLT to know the toxicity of the extracts
based on Mc Lauglin method. The fourth step was the examination of antihyperlipidemic activity of treatment I which was the combination of avocado and gedi leaves extract and treatment II avocado
and selada darat leaves extract. The result showed that treatment I possesed weak antihyperlipidemic effect p0,005 while treatment II had significant antihyperlipidemic activity that reduced
triacylgliseride and LDL but incerased HDL in mice. Phytochemicals analysis of the extracts showed that the plants consisted of flavonoids flavonol myrisetin, keemferol, quarsetin, saponins, tanins and
steroids. The LC
50
of BSLT was 300 µgml in avocado, 535 µgml in gedi and 424 µgml in selada
darat leaves extracts respectively.
Key words :
antihyperlipidemic, plant extracts, gedi leaf, selada darat leaf, avocado leaf
1. Introduction
