Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 502
2.6.2. Antifeedant Test
The inhibitory effect of eating or called by antifeedant calculated using the formula :
AF = [ Bk - Bp Bk + Bp ] x 100 Where,
AF = antifeedant effect, Bk = weight control leaves are eaten,
Bp = weight treatment leaves are eaten.
The difference between the weight of leaf treatment and weight control leaves are eaten analyzed by paired t test and antifeedant effect data were analyzed by analysis of variance
followed by Duncan test
2.6.3. Reproduction and Development Test
Data larval mortality and length of 1-5 instar larval stage were analyzed by analysis of variance followed by Duncan test.
4. Results and Discussion 4.1. Extraction and Partition
Extraction and partitioning of
Barringtonia asiatica
seeds per 1,000 grams of powder to produce the amount of crude extract were more presented in Table 1.
Table 1. Extraction and Partition Seed B. asiatica
Extract Plant HHeavy
Powder g Extraction and Partisis g
Crude extract Fraksi CH3OH 95
Fraksi EtOAc B. asiatica
1000 110,17 11 64,85 6,5
52,435,2
Seed
B. asiatica
as much as 1 kg of the test plants after dissolved with methanol CH 3 OH to produce crude extract each as much as 93.22 g and 110.17 g. Results partition the crude
extract with solvent hexane C6H14 - methanol CI3OH 95 respectively produce the methanol fraction of 95 amounting to 59.09 g and 64.85 g, then the result of partitioning the
methanol fraction 95 solvent ethyl acetate Et OAc - water H2O to produce ethyl acetate fractions of 43.27 g and 52.34 g. From the extraction and partitioning it turns out B. asiatica
extract to produce enough material ie about 16.95 g.
B. asiatica
seed extract contains active ingredients saponins having foaming properties in water and very soluble in water.
4.2. Toxicity Extracts Against Larvae of S. exigua
Results of testing the mortality of larvae of
S. exigua
treated with
B. asiatica
seed extract can be found in Appendix 1, which then created a list of the percentage of cumulative mortality of
larvae of
S. exigua
based day treatment as in Table 2.
Table 2. The Cumulative Mortality ofLlarvae of S. exigua by Day After Treatment
Consentration ____Larval Mortality Day __________ 1 2 3 4 5 6 7 8 9 10
B. asiatica 0 0 0 0 0 0 0 0 0 0 0
0,15 0 4 12 20 23 24 25 25 25 25 0,20 0 11 27 29 32 33 35 35 35 35
0,25 0 16 31 43 47 51 53 56 56 56 0,30 16 55 67 81 85 85 85 85 85 85
0,35 27 100 100 100 100 100 100 100 100 100
Information : m= Larvae mortality, Kf= cumulative mortality, Percentace Cumulative mortality
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