Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 495
analyzed bacterial isolates in this research was performed by construction dendrogram form Figure 2.
Figure 2. Dendrogram obtained by ARDRA profile of 2 strains lactic acid bacteria proteolytic and 2 reference strains member of Pediococcus genus generated from restriction by HaeIII enzyme restriction.
Dendrogram regerated from restriction by
Hae
III enzyme restriction in Figure 2 show that strains of B3.5 and B9.7 could performe to be member of the same species with similarity
index 100. Those isolates showed that they have the taxonomy or very close relationship. These two strains are within one cluster with reference strains of
Pediococcus acidilactici
0110 FNCC with similarity index 100, its means that the two strains are identical to the reference strain of
P ediococcus acidilactici
FNCC 0110. These results are consistant with data of phenotypic characteristics.
4. Conclusion
ARDRA is an accurate and reliable method capable of discriminating among the test strains B3.5 and B9.7 and reference strains
P. pentosaceus
FNCC 0019 and
Pediococcus acidilactici
FNCC 0110. The two strains B3.5 and B9.7 are identical to the reference strain of
Pediococcus acidilactici
FNCC 0110. These results are consistant with data of phenotypic characteristics. The procedure is based on digestion of the PCR amplicon amplified by 27f
and 1492r primers using two enzyme restriction endonuclease.
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Biology Page 497
BIOACTIVITY OF SEED EXTRACT BITUNG
Barringtonia asiatica
TO ONION WORM
Spodoptera exigua
HUBNER LEPIDOPTERA: NOCTUIDAE
Utari Satiman
1 1
Biology Department, Faculty of Mathematics and Natural Science, State University of Manado, Minahasa, Indonesia
utarisayahoo.com
Abstract
Spodoptera exigua
is one of the important pest on spring onion in Minahasa. Eforts are made to control the pest without any negative impact by using botanical insecticides. The aim of this research
was to assess : 1 the toxicity of EtOAc extract fraction of
Barringtonia asiatica
seed on
Spodoptera exigua
larvae 2 the influence of extract concentration on antifeedant activity of
S. Exigua
larvae and 3 the effect of extract sublethal concentration on the development and reproduction of
S. exigua
. Leaves residual method was used to evaluate the toxicity and the sublethal concentration extract to the
development and reproduction of
S. Exigua.
Second instar larvae of
S. Exigua
was used as a larvae test. The research was carried out using completely randomized design consisted of six treatments.
Each treatment was replicated five times. Probit analysis was used to calculate correlation between extract concentration and larvae mortality in order to determine toxicity LC
50
. Analysis varians was performed to calculate length of period of larvae, pupae, imago and female fecundity in regard to the
growth and reproduction of S.
exigua
. The effect of extract on antifeedant activity was determined using choice method. The result showed that
B. Asiatica
seed extract had insecticidal activity on LC
50
which was 0,21 . Extracted seed of
B. Asiatica
inhibited antifeedant activity of the test larvae at concentration 14.93
– 34.01.
B. Asiatica
extracted seed on sub lethal concentration of 0.15 to 0,30 could also extend length of period of second instar larvae to imago in average 1,92 to 2,45 days. Also,
it was reducing the percentation of pupae to be imago viz 94,60 as well as shorten living duration of male imago from 1.44 to 8,46. Whereas, female adult was 0,71 to 3 days. Furthermore, it was also
decreasing the number of eggs within a range of 41 to 112 eggs. Keywords :
Barringtonia asiatica
extract seed, toxicity, antifeedant,
Spodoptera exigua
1. Introduction 1.1. Backround
