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effect in the real against the decline of COD on the extent α of 0.05, So it no need be
forwarded test with.
5. Discussion 5.1. The Content of Ammonia
In this study the biomass of
Ipomea aquatica
may decrease the high ammonia content through the process of bioremediation of waste cattle pigs. The process of processing
pollutants in bioremediation, is through some process that is performed by the mikrobia and plants according to Hollerung, 2007; The 2010 Irawanto, Mangkoediharjo, 2010,
Supradata 2008 is as follows:
a. Biodegradation in Aerobicanaerobic metabolic process, is a microorganism that
effectively removes organic material b.
Phyto-uptake process and accumulation, accumulation of inorganic materials by the plant,
c. Phyto-stabilization, is the ability of some plants to separate water inorganic materials
on plant roots. d.
Phyto-degradation, the plant can produce enzymes that can break down organic materials or inorganic pollutants absorbed from, during the process of transpiration.
e. Rhizo-degradation, plant roots are able to perform material absorption of pollutants
of organic matter degradation results conducted by microbe. f.
Phyto-volatilitionevapotranspiration, absorption and transpiration Different phenomena that are found through the study is observational treatment 10 DAP,
where an increase in ammonia content in waste where the greater biomass treatment plants then become more ammonia content in waste water. The authors observed that the fact this is
because plants that serve as the treatment is having the symptoms of decay and some were starting to die, thus increasing the content of ammonia is no longer derived from livestock
waste but from the decay of plant water spinach.
5.2 BOD and COD
Research results through treatment biomass of
ipomea aquatica
against swine livestock waste overall decline in average levels of BOD and COD. The tendency of a decrease in the
concentrations of BOD and COD, because it is very related to the performance and growth of the existing microbe in the waste. This is according to Warmadewanthi 2001, that the
existence of processes in chemistry and biology through the activity of microorganisms or plants Bioremediation dissolved BOD COD can be removed.
Several things may explain the decline of organic material in the waste of those pigs, according to Wood in Tangahu Warmadewanthi 2001 that the decrease concentration of
organic and inorganic materials occurred due to the mechanism of activity of microorganisms and plants, through the process of oxidation by aerobic bacteria that grow around the
rhyzosphere of plants as well as the presence of heterotrophic bacteria in the waste water.
According to Brix in Khiatuddin 2003, stated that under the surface of the soil, the roots of aquatic plants, removing oxygen, so the rhyzosphere zone is formed that is rich in oxygen
throughout the surface of the hair roots. The flow of oxygen to the roots through the rod after diffusion from the atmosphere through the leaf pores. The opinion was reinforced with
Supradata 2008 that a release of oxygen surrounding the root rhyzosphere that is very possible because hydrophyta types of plants including water spinach plants, have spaces
Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 463
between cells or air duct holes aerenchyma as a means of transport of oxygen from the atmosphere to the rooting. According to Haberl and Langergraber 2002, that the process of
photosynthesis on aquatic plants hydrophyta including
Ipomea acuatica
, allows the release of oxygen in the area around rooting rhyzosphere zones. With conditions of rhyzosphere
zones rich in oxygen, aerobic bacteria led to the development in the zone. Based on the above, then the primary role of microorganisms degrade organic and inorganic materials in waste
pig, will be able to explain the trendtendency of decrease of BOD and COD.
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Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 464
VIABILITY AND MORPHOLOGY OF SPERMATOZOA IN HYPERLIPIDEMIA RAT
Rattus norvegicus
TREATED WITH SARANG SEMUT EXTRACT
Myrmecodia pendans
Merr. Perry
Djuna Lamondo
1
, Margaretha Solang
1
1
Department of Biology, Faculty of Mathematics and Natural Science, State University of Gorontalo, Gorontalo, Indonesia
juna_ungyahoo.co.id
Abstract Hyperlipidemia will increase Reactive Oxygen Species ROS and may cause lipid peroxidation in cell
membrane, decrease viability, and increase the chance of abnormality in spermatozoa. Sarang Semut
Myrmecodia pendans
contains active compound such as flavonoid, tannin, tocopherol, and polyphenol that may act as antioxidant. We hypothesize that these phytochemical compounds may
protect sperm cell from oxidative damage caused by ROS from hyperlipedemia condition. Thus, this study aimed to observe the changes in viability and morphology of spermatozoa in hyperlipidemia rat
treated with Sarang Semut extract. Post test control group design and completely randomized design were used in this experiment, consisted of five treatment groups with four replications. The treatment
goups were; Treatment group A control:standard food sf, Treatment group B: sf and yolk yl 1.5 gr 200 gr body weight BW, Treatment group C: sf, yl and extract Sarang Semut ESS dose 129 mg
200 gr BW, Treatment group D: sf, yl and ESS dose of 258 mg 200 gr BW, and treatment group E: sf, yl and ESS dose of 387 mg 200 gr BW. Blood cholesterol levels are measured using CHOD-
PAP, and spermatozoa were collected from the cauda epididymis prior to observation. Statistical test using F test showed that the ant plant extract affect viability but did not affect spermatozoas
morphology in hyperlipidemic rat. The highest increase of spermatozoas viability in hyperlipidemic rat were observed in the experiment group with ant plant extract with a dose of 129 mg BW rats for 2
weeks. Keywords: Spermatozoa,Viability, morphology, extract Sarang Semutt
1. Introduction
