Proceedings of MatricesFor IITTEP – ICoMaNSEd 2015
ISBN: 978-602-74204-0-3
Biology Page 499
2.2. Materials and Tools of Research
The tools used in this study include insect breeder box 50 x 50 x 50 cm, Oven, digital Balance GR - 200
δ = 0.1 mg, blender, Mohr pipette 0.5, 1, and 5 ml, beaker, flask, shaker magnetic stirrer TC - 2, rotary evaporator Buchi R - 144, cork borer O3 cm, vacuum pump
VP - 16, pumpkin filter, pumpkin separator, Buchner funnel, petri dish ø 9 and 20 cm, plastic box 50 x 30 x 10 cm, coarse and fine filter paper Whatman No. 41, ø 12.5 cm,
erlenmeyer flask, sieve ø 0.5 mm, aluminum foil, jars ø 18 cm, 300 ml glass jars, loupe, blotting paper, brushes, cotton, label paper, scissors, writing equipment and cameras.
Materials used include the larvae of S. exigua, seeds Bitung Barringtonia asiatica, plant scallion, mead 10 , methanol CH 3 OH, ethyl acetate EtOAc, hexane C6H14, distilled
water H2O, Agriwet 100 S, dregs saws and documentation material.
2.3 Research Method 2.3.1. Pengujian Toksisitas Ekstrak Terhadap Mortalitas Larva
S. exigua
The test method used is the residue on the leaves by way of immersion. Tests are arranged in a complete randomized design CRD, the six concentration and each treatment was repeated
five times for each extract. The treatment consisted of concentration of ethyl acetate fraction of methanol extract of seeds of
B. asiatica
0 , 0.15 , 0.20 , 0.25 , 0.30 and 0.35 . Each concentration was obtained based on preliminary test results that can cause mortality of
larvae of
S. exigua
between 0-100 .
2.3.2.
B. asiatica
Seed Extract Influence on the Activities of Eating
This testing is done by the method of residue on the leaves by way of choice. The experiment was arranged in a completely randomized design CRD, six treatments and five replications.
The treatment consisted of LC0 control, LC15, LC35, LC55, LC75 and LC100 from ethyl acetate fraction of methanol extract of seeds of
B. asiatica
.
2.3.3. Effect of Extract Against Development and Reproduction
S. exigua
Method is a method of testing conducted residue on the leaves by way of immersion. The experiment was arranged in a complete randomized design CRD with six treatments and
five replications. The treatment consisted of concentration of ethyl acetate fraction of methanol extract of seeds of
B. asiatica
0 , 0.15 , 0.20 , 0.25 , 0.30 , 0.35 . Each concentration was obtained based on those preliminary test results that can cause mortality of
larvae of
S. exigua
between 0-100 . The population in each treatment increased the amount to be used as a material in further treatment, especially in determining the reproduction of the
imago.
2.4. Research Procedure 2.4.1. Propagation Insect
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