55 Lampiran 3. Data hasil analisis sidik ragam dan uji lanjut Duncan
A. Data hasil analisis sidik ragam dan uji lanjut Duncan penentuan konsentrasi kawao
Larutan baku standar glukosa+fruktosa 10mM, volume total 2ml.
Volume
µ
L [glu+fru]
µ
M abs
0,000 30
150 0,260
45 225
0,388 75
375 0,472
105 525
0,687 135
675 0,798
0,1 0,2
0,3 0,4
0,5 0,6
0,7 0,8
0,9
100 200
300 400
500 600
700 800
Konsentrasi glukosa+fruktosa uM abs
Gambar 30. Kurva standar glukosa+fruktosa, dengan nilai y = 0.0011x – 0.0624
dan r
2
= 0.9725.
kons kawao,
vv Abs 1
Abs 2 Kons 1
Kons 2 Kons
rata- rata
0,674 0,927
556,000 786,000
671,000 2,5
0,221 0,314
144,182 228,727
186,455 5
0,161 0,192
89,636 117,818
103,727 10
0,183 0,21
109,636 134,182
121,909 15
0,266 0,245
185,091 166,000
175,545 20
0,328 0,294
241,455 210,545
226,000 25
0,475 0,358
375,091 268,727
321,909
56
ANOVA INVERT
459831,5 6
76638,580 14,484
,001 37038,892
7 5291,270
496870,4 13
Between Groups Within Groups
Total Sum of
Squares df
Mean Square F
Sig.
Between-Subjects Factors
2 2.5
2 5
2 10
2 15
2 20
2 25
2 1,00
2,00 3,00
4,00 5,00
6,00 7,00
KONS Value Label
N
Tests of Between-Subjects Effects
Dependent Variable: INVERT 459831,480
a
6 76638,580
14,484 ,001
932458,525 1
932458,525 176,226
,000 459831,480
6 76638,580
14,484 ,001
37038,892 7
5291,270 1429328,896
14 496870,371
13 Source
Corrected Model Intercept
KONS Error
Total Corrected Total
Type III Sum of Squares
df Mean Square
F Sig.
R Squared = ,925 Adjusted R Squared = ,862 a.
INVERT
Duncan
a,b
2 103,72700 2 121,90900
2 175,54550 175,54550
2 186,45450 186,45450
2 226,00000 226,00000
2 321,90900
2 671,00000
,159 ,099
1,000 KONS
5 10
15 2.5
20 25
Sig. N
1 2
3 Subset
Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares
The error term is Mean SquareError = 5291,270. Uses Harmonic Mean Sample Size = 2,000.
a. Alpha = ,05.
b.
57
B. Data hasil analisis sidik ragam dan uji lanjut Duncan pengaruh konsentrasi enzim
Larutan baku standar glukosa+fruktosa 10mM, volume total 2ml.
Volume µL
[glu+fru] µM
abs Volume
µL [glu+fru]
µM abs
0,034 120
600 0,212
0,033 135
675 0,253
30 150
0,034 135
675 0,250
30 150
0,033 150
750 0,294
45 225
0,035 150
750 0,293
45 225
0,037 180
900 0,388
75 375
0,098 180
900 0,375
75 375
0,091 250
1250 0,582
90 450
0,124 250
1250 0,568
90 450
0,134 300
1500 0,702
120 600
0,210 300
1500 0,702
-0,1 0,1
0,2 0,3
0,4 0,5
0,6 0,7
0,8
200 400
600 800
1000 1200
1400 1600
Konsentrasi glukosa+fruktosa uM abs
Gambar 31. Kurva standar glukosa+fruktosa, dengan nilai y = 0.0005x – 0.0530
dan r
2
= 0.9735.
1. Kondisi tanpa penambahan kawao
Kons enzim
mgl Abs 1
Abs 2 Kons 1
Kons 2 Kons
rata-rata
106,000 106,000
106,000 0,15
106,000 106,000
106,000 0,85
106,000 106,000
106,000 1,65
0,068 0,058
242,000 222,000
232,000 2,5
0,296 0,31
698,000 726,000
712,000 3,35
0,591 0,587
1288,000 1280,000
1284,000 4,15
0,744 0,764
1594,000 1634,000
1614,000 4,5
0,898 0,885
1902,000 1876,000
1889,000
58
ANOVA KONS
4751799 6
791966,476 3893,094
,000 1424,000
7 203,429
4753223 13
Between Groups Within Groups
Total Sum of
Squares df
Mean Square F
Sig.
Between-Subjects Factors
2 0.15
2 0.85
2 1.65
2 2.5
2 3.35
2 4.15
2 1
2 3
4 5
6 7
ENZIM Value Label
N
Tests of Between-Subjects Effects
Dependent Variable: KONS 4751798,857
a
6 791966,476
3893,094 ,000
4944457,143 1 4944457,143
24305,618 ,000
4751798,857 6
791966,476 3893,094
,000 1424,000
7 203,429
9697680,000 14
4753222,857 13
Source Corrected Model
Intercept ENZIM
Error Total
Corrected Total Type III Sum
of Squares df
Mean Square F
Sig.
R Squared = 1,000 Adjusted R Squared = ,999 a.
KONS
Duncan
a,b
2 106,000
2 106,000
2 106,000
2 232,000
2 712,000
2 1284,000
2 1614,000
1,000 1,000
1,000 1,000
1,000 ENZIM
0.15 0.85
1.65 2.5
3.35 4.15
Sig. N
1 2
3 4
5 Subset
Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares
The error term is Mean SquareError = 203,429. Uses Harmonic Mean Sample Size = 2,000.
a. Alpha = ,05.
b.
59 2. Kondisi dengan penambahan kawao
Kons enzim
mgl Abs 1
Abs 2 Kons 1
Kons 2 Kons
rata-rata
0,054 0,054
214,000 214,000
214,000 0,15
0,073 0,065
252,000 236,000
244,000 0,85
0,073 0,077
252,000 260,000
256,000 1,65
0,075 0,082
256,000 270,000
263,000 2,5
0,084 0,082
274,000 270,000
272,000 3,35
0,131 0,134
368,000 374,000
371,000 4,15
0,185 0,187
476,000 480,000
478,000 4,5
0,230 0,222
566,000 550,000
558,000 Perhitungan konsentrasi mengacu pada persamaan kurva standar di Gambar 31.
ANOVA KONS
102690,9 6
17115,143 410,295
,000 292,000
7 41,714
102982,9 13
Between Groups Within Groups
Total Sum of
Squares df
Mean Square F
Sig.
Between-Subjects Factors
2 0.15
2 0.85
2 1.65
2 2.5
2 3.35
2 4.15
2 1
2 3
4 5
6 7
ENZIM Value Label
N
Tests of Between-Subjects Effects
Dependent Variable: KONS 102690,857
a
6 17115,143
410,295 ,000
1257601,143 1 1257601,143
30147,973 ,000
102690,857 6
17115,143 410,295
,000 292,000
7 41,714
1360584,000 14
102982,857 13
Source Corrected Model
Intercept ENZIM
Error Total
Corrected Total Type III Sum
of Squares df
Mean Square F
Sig.
R Squared = ,997 Adjusted R Squared = ,995 a.
60
KONS
Duncan
a,b
2 214,000
2 244,000
2 256,000
256,000 2
263,000 263,000
2 272,000
2 371,000
2 478,000
1,000 ,106
,314 ,206
1,000 1,000
ENZIM 0.15
0.85 1.65
2.5 3.35
4.15 Sig.
N 1
2 3
4 5
6 Subset
Means for groups in homogeneous subsets are displayed. Based on Type III Sum of Squares
The error term is Mean SquareError = 41,714. Uses Harmonic Mean Sample Size = 2,000.
a. Alpha = ,05.
b.
C. Data hasil analisis sidik ragam dan uji lanjut Duncan pengaruh konsentrasi substrat
