Analisis Marka SSR Bahan dan Metode

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4.4 Kesimpulan

Primer BamCo80 adalah primer yang cukup polimorfik dan informatif dibandingkan primer lainnya dengan nilai PIC 0.453. Keragaman genetik kacang bogor asal Sumedang lebih tinggi 0.119 daripada Sukabumi 0.020. Berdasarkan lokus yang dianalisis, sampel terbagi kedalam dua kelompok utama, pengelompokan sampel berdasarkan pada daerah asal Sukabumi dan Sumedang. Daftar Pustaka Agarwal M, Shrivastava N, Padh H. 2008. Advances in molecular marker techniques and their applications in plant sciences. Plant Cell Rep. 274:617-631. Amadou HI, Bebeli PJ, Kaltsikes PJ. 2001. Genetic diversity in Bambara groundnut Vigna subterranea L. germplasm revealed by RAPD markers. Genome. 446:995-9. Basu S, Roberts JA, Azam-Ali SN, Mayes S. 2007. Bambara groundnut. Di dalam: Kole C, editor. Genome Mapping and Molecular Breeding in Plants. Volume 3: Pulses, Sugar and Tuber Crops. Pennsylvania State University US: Springer Verlag Berlin Heidelberg. hlm 159-173. Boer D. 2007. Keragaman dan Struktur Genetik Populasi Jati Sulawesi Tenggara Berdasarkan Marka Mikrosatelite. [disertasi]. Bogor ID: Institut Pertanian Bogor. Das BK, Jena RC, Samal KC. 2009. Optimization of DNA isolation and PCR protocol for RAPD analysis of bananaplantain Musa spp.. Int J Agricul Sci. 12:21-25. Gupta PK, Roy JK, Prasad M. 2001. Single nucleotide polymorphisms: A new paradigm for molecular marker technology and DNA polymorphism detection with emphasis on their use in plants. Current Sci. 804:524-535. Hampson K, Azam-Ali SH, Sesay A, Mukwaya SM, Azam-Ali SN. 2000. Assessing opportunities for increased utilisation of bambara groundnut in southern africa. Loughborough UK: University of Nottingham. hlm 67. Gambar 4.6 Struktur populasi kacang bogor asal Sukabumi dan Sumedang. delta K = 2. 1=SKC, 2=OGN, 3=SKH, 4=SMP dan 5=SMH. 47 Hillocks RJ, Bennett C, Mponda OM. 2012. Bambara nut: a review of utilisation, market potential and crop improvement. African Crop Science Journal. 201:1-16. Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK. 2011. Microsatellite markers: an overview of the recent progress in plants. Euphytica. 1773:309-334. Kouassi NJ, Zoro BIA. 2010. Effect of sowing density and seedbed typed on yield and yield components in bambara groundnut Vigna subterranea in wo odland savannas of Côte d’Ivore. Experimental Agriculture. 461:99- 110. Marshall TC, Slate J, Kruuk LEB, Pemberton JM. 1998. Statical confidence for likelihood-based paternity inference in natural populations. Mol. Ecol. 75:639-655. Massawe FJ, Dickinson M, Roberts JA, Azam-Ali SN. 2002. Genetic diversity in Bambara groundnut Vigna subterranea L. Verdc. landraces revealed by AFLP markers. Genome. 456:1175-1180. Mkandawire CH. 2007. Review of bambara groundnut production in sub-Saharan Africa. Agricultural Journal. 24:464-470. Opara UL, Jacobson D, Al-Saady NA. 2010. Analysis of genetic diversity in banana cultivars Musa cvs. from the South of Oman using AFLP markers and classification by phylogenetic, hierarchical clustering and principal component analyses. J Zhejiang Univ Sci. 115:332-341. Peakall R, Smouse PE. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic software for teaching and research – an update. Bioinformatics. 2819:2537-2539. Perrier X, Flori A, Bonnot F. 2003. Data analysis methods. In: Hamon, P., Seguin, M., Perrier, X., Glaszmann, J. C. Eds., Genetic diversity of cultivated tropical plants. Enfield, Science Publishers. Montpellier. hlm 43 –76. Pritchard JK, Wen X, Falush D. 2007. Documentation for STRUCTURE software: Version 2.3.4, available from http:pritch.bsd.uchicago.edu softwarestructure 2.3.4readme.pdf [accessed 15 Juni 2015]. Rao NK. 2004. Plant genetic resources: Advancing conservation and use through biotechnology. African J. Biotech. 32:13-145. Redjeki ES. 2003. Pengaruh seleksi galur murni pada populasi campuran terhadap hasil tanaman kacang bogor Vigna subterranea L. Verdcourt. Agrofish. 32:97-105. Rungnoi O, Suwanprasert J, Somta P, Srinives P. 2012. Molecular genetic diversity of Bambara groundnut Vigna subterranea L. Verdc. revealed by RAPD and ISSR marker analysis. SABRAO J. Breed. Genet. 441:87-101. Sajib AM, Hosain MM, Moznas ATMJ, Hosain H, Islam MM, Ali MS, Prodhan SH. 2012. SSR marker-based molecular characterization and genetic diversity analysis of aromatik landraces of rice Oryza sativa L.. J. BioSci Biotech. 12:107-116. Siise A, Massawe FJ. 2012. Microsatellites based marker molecular analysis of Ghanian Bambara groundnut Vigna subterranea L. Verdc. landraces alongside morphological characterization. Genet Resour Crop Evol. 602:777-787.