46
4.4 Kesimpulan
Primer  BamCo80  adalah  primer  yang  cukup  polimorfik  dan  informatif dibandingkan primer lainnya dengan nilai PIC 0.453. Keragaman genetik kacang
bogor  asal  Sumedang  lebih  tinggi  0.119  daripada  Sukabumi  0.020. Berdasarkan lokus yang dianalisis, sampel terbagi kedalam dua kelompok utama,
pengelompokan sampel berdasarkan pada daerah asal Sukabumi dan Sumedang.
Daftar Pustaka
Agarwal  M,  Shrivastava  N,  Padh  H.  2008.  Advances  in  molecular  marker techniques  and  their  applications  in  plant  sciences.  Plant  Cell  Rep.
274:617-631. Amadou  HI,  Bebeli  PJ,  Kaltsikes  PJ.  2001.  Genetic  diversity  in  Bambara
groundnut  Vigna  subterranea  L.  germplasm  revealed  by  RAPD  markers. Genome. 446:995-9.
Basu  S,  Roberts  JA,  Azam-Ali  SN,  Mayes  S.  2007.  Bambara  groundnut.  Di dalam: Kole C, editor. Genome Mapping and Molecular Breeding in Plants.
Volume  3:  Pulses,  Sugar  and  Tuber  Crops.  Pennsylvania  State  University US: Springer Verlag Berlin Heidelberg. hlm 159-173.
Boer D. 2007. Keragaman dan Struktur Genetik Populasi Jati Sulawesi Tenggara Berdasarkan Marka Mikrosatelite. [disertasi]. Bogor ID: Institut Pertanian
Bogor. Das  BK,  Jena  RC,  Samal  KC.  2009.  Optimization  of  DNA  isolation  and  PCR
protocol  for  RAPD  analysis  of  bananaplantain  Musa  spp..  Int  J  Agricul Sci. 12:21-25.
Gupta  PK,  Roy  JK,  Prasad  M.  2001.  Single  nucleotide  polymorphisms:  A  new paradigm  for  molecular  marker  technology  and  DNA  polymorphism
detection with emphasis on their use in plants. Current Sci. 804:524-535. Hampson  K,  Azam-Ali  SH,  Sesay  A,  Mukwaya  SM,  Azam-Ali  SN.  2000.
Assessing  opportunities  for  increased  utilisation  of  bambara  groundnut  in southern africa. Loughborough UK: University of Nottingham. hlm 67.
Gambar 4.6  Struktur populasi kacang bogor asal Sukabumi dan Sumedang. delta K = 2. 1=SKC, 2=OGN, 3=SKH, 4=SMP dan 5=SMH.
47 Hillocks RJ, Bennett C, Mponda OM. 2012. Bambara nut: a review of utilisation,
market  potential  and  crop  improvement.  African  Crop  Science  Journal. 201:1-16.
Kalia RK, Rai MK, Kalia S, Singh R, Dhawan AK. 2011. Microsatellite markers: an overview of the recent progress in plants. Euphytica. 1773:309-334.
Kouassi NJ, Zoro BIA. 2010. Effect of sowing density and seedbed typed on yield and  yield  components  in  bambara  groundnut  Vigna  subterranea  in
wo odland  savannas  of  Côte  d’Ivore.  Experimental  Agriculture.  461:99-
110. Marshall  TC,  Slate  J,  Kruuk  LEB,  Pemberton  JM.  1998.  Statical  confidence  for
likelihood-based  paternity  inference  in  natural  populations.  Mol.  Ecol. 75:639-655.
Massawe FJ, Dickinson M, Roberts JA, Azam-Ali SN. 2002. Genetic diversity in Bambara  groundnut  Vigna  subterranea  L.  Verdc.  landraces  revealed  by
AFLP markers. Genome. 456:1175-1180. Mkandawire CH. 2007. Review of bambara groundnut production in sub-Saharan
Africa. Agricultural Journal. 24:464-470. Opara  UL,  Jacobson  D,  Al-Saady  NA.  2010.  Analysis  of  genetic  diversity  in
banana cultivars Musa cvs. from the South of Oman using AFLP markers and  classification  by  phylogenetic,  hierarchical  clustering  and  principal
component analyses. J Zhejiang Univ Sci. 115:332-341.
Peakall R, Smouse PE. 2012. GenAlEx 6.5: genetic analysis in Excel. Population genetic  software  for  teaching  and  research
–  an  update.  Bioinformatics. 2819:2537-2539.
Perrier X, Flori A, Bonnot F. 2003. Data analysis methods. In: Hamon, P., Seguin, M.,  Perrier,  X.,  Glaszmann,  J.  C.  Eds.,  Genetic  diversity  of  cultivated
tropical plants. Enfield, Science Publishers. Montpellier. hlm 43 –76.
Pritchard  JK,  Wen  X,  Falush  D.  2007.  Documentation  for  STRUCTURE software:  Version  2.3.4,  available  from  http:pritch.bsd.uchicago.edu
softwarestructure 2.3.4readme.pdf [accessed 15 Juni 2015]. Rao NK. 2004. Plant genetic resources: Advancing conservation and use through
biotechnology. African J. Biotech. 32:13-145. Redjeki ES. 2003. Pengaruh seleksi galur murni pada populasi campuran terhadap
hasil  tanaman  kacang  bogor  Vigna  subterranea  L.  Verdcourt.  Agrofish. 32:97-105.
Rungnoi  O,  Suwanprasert  J,  Somta  P,  Srinives  P.  2012.  Molecular  genetic diversity of Bambara groundnut Vigna subterranea L. Verdc. revealed by
RAPD and ISSR marker analysis. SABRAO J. Breed. Genet. 441:87-101. Sajib AM, Hosain MM, Moznas ATMJ, Hosain H, Islam MM, Ali MS, Prodhan
SH.  2012.  SSR  marker-based  molecular  characterization  and  genetic diversity analysis of aromatik landraces of rice Oryza sativa  L.. J. BioSci
Biotech. 12:107-116.
Siise  A,  Massawe  FJ.  2012.  Microsatellites  based  marker  molecular  analysis  of Ghanian  Bambara  groundnut  Vigna  subterranea  L.  Verdc.  landraces
alongside  morphological  characterization.  Genet  Resour  Crop  Evol. 602:777-787.