16
2.7 Gen Pto
Gen yang mengendalikan sifat ketahanan terdiri atas dua kelompok yaitu: kelompok gen yang terlibat dalam pengenalan patogen atau sinyal transduksi
disebut gen ketahanan R gene; kelompok lainnya yang terlibat dalam mekanisme ketahanan dan sintesis produk yang dibutuhkan untuk pengenalan patogen
Chinchilla et al. 2006.
Berdasarkan domain structural yang dimiliki, gen ketahanan R gene dapat dikelompokkan menjadi tiga kelompok: 1 gen yang menyandi protein yang
mengandung Nucleotide Binding Site dan Leucine-Rich Repeat NBS-LRR; 2 gen yang menyandi protein yang mengandung SerinThreonin Kinase STK; 3
gen yang menyandi protein yang mengandung extracitoplasmic Leucine-Rich Repeat eLRR Gao et al. 2005. Berbagai gen ketahanan telah berhasil diisolasi
dari beberapa tanaman seperti kacang tanah Yuksel et al. 2005, kapas Gao et al. 2005, sorghum Totad et al. 2005, pisang Miller et al. 2008, tomat Pedley dan
Martin 2003, gandum Zhang et al. 2011 dan apel Baek dan Choi 2013.
Gen pto adalah sebuah protein kinase yang hanya mengandung Ser-Thr Kinase tanpa LRR Kim et al. 2002 dan Rpg1 yang mengkode receptor kinase-
like protein dengan 2 protein kinase Brueggeman et al. 2002. Kedua gen ini merupakan kelompok R gene yang berinteraksi secara langsung dengan masing-
masing penyebab serangan penyakit Wu et al. 2004. Gen Pto membutuhkan protein NBARC-LRR Prf untuk berfungsi Salmeron et al. 1996.
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