61 Sehubungan dengan pengembangan marka SNAP untuk marka ketahanan
terhadap penyakit, marka molekuler berbasis SNP sebaiknya dikembangkan dari beberapa gen yang mempunyai peranan dalam ketahanan terhadap penyakit.
Sebagai contoh implementasi marka SNAP berbasis SNP yang berasal dari gen Pto, dapat digabungkan dengan SNP yang berasal dari gen chitinase dan
β-1,3- glucanase Sutanto et al. 2013.
5.4 Kesimpulan
Fragmen gen Pto asal kacang bogor yang berukuran 555 pb, berhasil diidentifikasi adanya 22 posisi SNP, tetapi hanya 6 posisi SNP yang digunakan
untuk mendisain primer SNAP. Berdasarkan 6 posisi SNP terpilih, dihasilkan 12 pasang primer SNAP untuk pengembangan marka SNAP kacang bogor. Sebanyak
12 pasang primer yang didapat, 9 pasang primer SNAP terbukti efektif untuk mengamplifikasi DNA genom kacang bogor, sedangkan 3 pasang primer SNAP
lainnya tidak berhasil mengamplifikasi DNA genom kacang bogor. Berdasarkan lokus SNP_78, SNP_378, SNP_481 and SNP_510, genotipe kacang bogor
mempunyai kombinasi alel yang heterozigot, dan satu lokus SNP_502 mempunyai kombinasi alel yang homozigot.
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