UIN Syarif Hidayatullah Jakarta
BAB 5 KESIMPULAN DAN SARAN
5.1 Kesimpulan
1. Kondisi optimum untuk analisis timokuinon dalam minyak biji jinten hitam
menggunakan sistem kromatografi terdiri dari kolom Acclaim
®
Polar Advantage II C18 dengan kecepatan alir 1,5 mLmenit, detektor UV
dengan panjang gelombang 252 nm, volume penyuntikan 20 μL dengan
sistem isokratik pada komposisi eluen metanol : air 70:30 2.
Validasi metode yang dilakukan memberikan hasil nilai linearitas r =
0,9997 pada rentang 0,5 – 500 μgml. Batas deteksi dan batas kuantitasi
8,67 μgmL dan 28,9 μgmL, diff sekitar -1,864 sampai 1,562, presisi
RSD berkisar 0,052 sampai 0,113 dan perolehan kembali 98,135
sampai 101,563 . 3.
Analisis timokuinon pada sampel minyak biji jinten hitam memiliki kadar
sebesar 3,968 bb.
5.2 Saran
Perlu dilakukan pengembangan metode analisis Timokuinon dalam suatu sediaan atau pengembangan metode analisis dengan instrumen lain,
misalnya GCMS Gas Chromatography Mass Spectrometry.
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UIN Syarif Hidayatullah Jakarta
DAFTAR PUSTAKA
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Benhaddou- Andaloussi , A., L.C. Martineau, D.spoor,T. Vuong, C. Leduce, E.joly, A. Burt, B.meddah , A.settaf , J.T arnason, M.prenkti, P.S. Haddad. 2008.
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