Molecular epidemiology of avian inluenza virus infection in Indonesia

130 Sekretariat Perizinan Penelitian Asing Kementerian Riset dan Teknologi DIREKTORI PENELITIAN ASING DI INDONESIA 2013

76. Molecular epidemiology of avian inluenza virus infection in Indonesia

Tujuan Penelitian : Meneliti epidemiologi molekuler dari infeksi virus lu burung pada unggas, hewan, dan manusia untuk mengontrol infeksi virus tersebut pada manusia Bidang Penelitian : Mikrobiologi Daerah Penelitian : Jatim Surabaya, Sidoarjo, Malang Lama Penelitian : 12 dua belas bulan mulai 3 Juni 2013 Mitra Kerja : Lembaga Penyakit Tropis Universitas Airlangga Prof. Dr. Nasronudin Abstract The Indonesia-Japan collaborative project to establish the “Collaborative Research Center for Emerging and Re-emerging Infectious Diseases” CRC-ERID has been approved and supported by the Ministry of Education, Culture, Sports, Science and Technology, Japan, and now in progress since April 2007. CRC-ERID is established in Institute of Tropical Disease ITD, Airlangga University, and the collaborative research will continue until 31 st March 2015. The objectives of research are to advance molecular epidemiology of avian inluenza virus infection in birds, animals, and humans and to control human infection of the virus in Indonesia. All the research activities on avian inluenza will be conducted in Institute of Tropical Disease ITD, Airlangga University, in collaboration with Dr. Laksmi Wulandari, the head of the Avian Inluenza research group at ITD, Prof. Nasronudin, Director of ITD, and their colleagues. Prof. Kazufumi Shimizu, a Japanese long-term researcher who stays at ITD to carry out research on avian inluenza, will be dispatched from Center for Infectious Diseases CID, Kobe University Graduate School of Medicine. Also, Japanese collaborators of the long-term researcher Prof. Yoshitake Hayashi and Prof. Hak Hotta andor their colleagues will be dispatched from CfD, Kobe University for a short-term stay to join in the collaborative research activities at ITD. 131 Sekretariat Perizinan Penelitian Asing Kementerian Riset dan Teknologi DIREKTORI PENELITIAN ASING DI INDONESIA 2013 The following experiments on avian inluenza virus will be conducted. 1 The entire genome of some representative strains of highly pathogenic H5N1 avian inluenza virus obtained from chicken, wild birds and pigs as well as that of avirulent strains will be sequenced and compared with each other. 2 The hemagglutinin HA and the neuraminidase NA genes of the remaining strains obtained from chicken and pigs will be sequenced and compared. 3 The binding ainity of the above inluenza virus strains to the viral receptors of the human ct2-6 and the avian types a2-3 will be examined. The possible relationship between the receptor binding ainity and a mutations of the virus will be comparatively studied. 4 Heamagglutination inhibiting HI and neutralizing NT antibodies against H5N1 inluenzavirus as well as seasonal inluenza viruses H1N1 and H3N2, etc. in the sera of the above mentioned animals and humans patients, suspected cases and healthy people will be measured by HI and NT tests.

76.1 Prof. Kazufumi Shimizu