5 SIMPULAN DAN SARAN
Dari hasil dan bahasan penelitian dapat dibuat simpulan dan saran sebagai berikut:
5.1 Simpulan
- Kadar florotanin S. echinocarpum yang terkandung dalam ekstrak berkisar 4,25 – 12,16 mg floroglusinolg ekstrak S. echinocarpum. Ekstrak metanol
mempunyai aktivitas antioksidan paling kuat. Senyawa aktif yang terkandung dalam ekstrak metanol S. echinocarpum adalah floroglusinol
dan bifuhalol. - Ekstrak metanol S. echinocarpum tergolong relatif tidak toksik dan aman
dikonsumsi pada dosis 625 mgkg BB atau kurang. - Ekstrak metanol S. echinocarpum dapat memberikan efek hipoglikemik,
penurun kadar lipid peroksida serum, peningkat aktivitas ekstra selular superoksida dismutase, katalase, dan glutation peroksidase serum serta
profil kandungan Cu,Zn-SOD ginjal dan hati pada tikus diabetes melitus. - Ekstrak metanol S. echinocarpum dapat meningkatkan vasorelaksasi,
sensitivitas muskarinik reseptor sel endotelium, dan rasio sel endotelium aorta tikus diabetes melitus.
5.2 Saran Perlu penelitian lebih lanjut tentang:
- Ketersediaan bioavailability ekstrak metanol S. echinocarpum, floroglusinol, dan bifuhalol.
- Toksikokinetik dan toksikodinamik ekstrak metanol S. echinocarpum, floroglusinol, dan bifuhalol.
- Pemanfaatan ekstrak metanol S. echinocarpum, floroglusinol, dan bifuhalol sebagai komponen pangan fungsional.
105
LAMPIRAN
106
107 Lampiran 1 Sertifikat hasil identifikasi rumput laut coklat
108 Lampiran 2 Rendemen ekstraksi
Data berat sampel dan berat ekstrak
Perlakuan Berat sampel gram
1 2
3 Etanol
Metanol Aseton 70
Etanol 80 Metanol 80
Akuades 15,28
20,15 17,88
18,75 18,66
18,24 19,75
21,55 18,65
19,54 18,78
19,42 20,65
19,98 19,04
19,23 19,65
17,86 22,01
20,05 18,56
18,99 19,43
17,23 18,97
19,89 18,42
20,21 19,87
16,98 19,65
20,21 17,56
18,93 18,68
19,56
Perlakuan Berat ekstrak gram
1 2
3 Etanol
Metanol Aseton 70
Etanol 80 Metanol 80
Akuades 0,4523
0,9894 1,5985
1,2075 1,3752
4,3448 0,5846
1,0581 1,6673
1,2584 1,3841
4,6258 0,6422
0,9650 1,7041
1,2442 1,4423
4,4436 0,6845
0,9684 1,6611
1,2287 1,4262
4,2868 0,5843
0,9388 1,6394
1,3258 1,4823
4,0786 0,6052
0,9539 1,5628
1,2418 1,3935
4,6983
Data rendemen ekstrak rumput laut coklat S. echinocarpum
Perlakuan Ulangan
Rerata I
II III
Etanol Metanol
Aseton 70 Etanol 80
Metanol 80 Akuades
2,96 4,91
8,94 6,44
7,37
23,82 3,11
4,83 8,95
6,47 7,34
24,88 3,08
4,72 8,90
6,56 7,46
24,02 3,05
4,82 8,93
6,49 7,39
24,24
Analisis keragaman pengaruh pelarut terhadap rendemen
Descriptives
Rendemen N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Minimum
Maximum Lower Bound
Upper Bound 1
3 3.050000E0
.0793725 .0458258
2.852828 3.247172
2.9600 3.1100
2 3
4.820000E0 .0953939
.0550757 4.583028
5.056972 4.7200
4.9100 3
3 8.930000E0
.0264575 .0152753
8.864276 8.995724
8.9000 8.9500
4 3
6.490000E0 .0624500
.0360555 6.334866
6.645134 6.4400
6.5600 5
3 7.390000E0
.0624500 .0360555
7.234866 7.545134
7.3400 7.4600
6 3
2.424000E1 .5632051
.3251666 22.840921
25.639079 23.8200
24.8800 Total
18 9.153333E0
7.2043606 1.6980841E0
5.570689 12.735978
2.9600 24.8800
ANOVA Rendemen
Sum of Squares df
Mean Square F
Sig. Between Groups
881.666 5
176.333 3.102E3
.000 Within Groups
.682 12
.057 Total
882.348 17
109 Post Hoc Tests
Multiple Comparisons
Rendemen LSD
I Pelarut
J Pelarut
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Etanol
Metanol -1.77000
.19468 .000
-2.3647 -1.1753
Aseton 70 -3.44000
.19468 .000
-4.0347 -2.8453
Etanol 80 -4.34000
.19468 .000
-4.9347 -3.7453
Metanol 80 -5.88000
.19468 .000
-6.4747 -5.2853
Akuades -21.19000
.19468 .000
-21.7847 -20.5953
Metanol Etanol
1.77000 .19468
.000 1.1753
2.3647 Aseton 70
-1.67000 .19468
.000 -2.2647
-1.0753 Etanol 80
-2.57000 .19468
.000 -3.1647
-1.9753 Metanol 80
-4.11000 .19468
.000 -4.7047
-3.5153 Akuades
-19.42000 .19468
.000 -20.0147
-18.8253 Aseton
70 Etanol
3.44000 .19468
.000 2.8453
4.0347 Metanol
1.67000 .19468
.000 1.0753
2.2647 Etanol 80
-.90000 .19468
.001 -1.4947
-.3053 Metanol 80
-2.44000 .19468
.000 -3.0347
-1.8453 Akuades
-17.75000 .19468
.000 -18.3447
-17.1553 Etanol
80 Etanol
4.34000 .19468
.000 3.7453
4.9347 Metanol
2.57000 .19468
.000 1.9753
3.1647 Aseton 70
.90000 .19468
.001 .3053
1.4947 Metanol 80
-1.54000 .19468
.000 -2.1347
-.9453 Akuades
-16.85000 .19468
.000 -17.4447
-16.2553 Metanol
80 Etanol
5.88000 .19468
.000 5.2853
6.4747 Metanol
4.11000 .19468
.000 3.5153
4.7047 Aseton 70
2.44000 .19468
.000 1.8453
3.0347 Etanol 80
1.54000 .19468
.000 .9453
2.1347 Akuades
-15.31000 .19468
.000 -15.9047
-14.7153 Akuades Etanol
21.19000 .19468
.000 20.5953
21.7847 Metanol
19.42000 .19468
.000 18.8253
20.0147 Aseton 70
17.75000 .19468
.000 17.1553
18.3447 Etanol 80
16.85000 .19468
.000 16.2553
17.4447 Metanol 80
15.31000 .19468
.000 14.7153
15.9047 . The mean difference is significant at the 0.01 level.
110
y = 0.1044x - 0.1517 R
2
= 0.9702
0.1 0.2
0.3 0.4
0.5 0.6
0.7 0.8
0.9 1
20 40
60 80
100 120
140 160
180 200
Floroglusinol ppm
A bs
o rba
ns i
Lampiran 3 Kadar florotanin Data pengamatan absorbansi floroglusinol
Konsentrasi ppm 20
40 60
80 100
120 140
160 180
200
Absorbansi 0,034
0,039 0,100
0,094 0,136
0,142 0,196
0,192 0,291
0,276 0,465
0,471 0,582
0,572 0,652
0,652 0,823
0,835 0,940
0,955
Persamaan hubungan linier antara konsentrasi floroglusinol dan absorbansi
Data serapan ekstrak S. echinocarpum
Perlakuan Ulangan
I II
III Etanol
Metanol Aseton 70
Etanol 80 Metanol 80
Akuades 0,063
0,064 0,218
0,209 0,266
0,264 0,470
0,480 0,256
0,256 0,425
0,415 0,075
0,078 0,187
0,194 0,264
0,268 0,496
0,489 0,244
0,248 0,409
0,409 0,070
0,072 0,198
0,199 0,265
0,266 0,485
0,480 0,250
0,252 0,410
0,415
Data kadar florotanin ekstrak S. echinocarpum yang terlarut dalam berbagai pelarut.
Perlakuan Florotanin
eq mg floroglusinol g ekstrak Rerata
I II
III Etanol
Metanol Aseton 70
Etanol 80 Metanol 80
Akuades 4,1130
4,1322 7,0824
6,9099 11,9099
12,1015 7,8103
7,8103 8,0019
7,9636 11,0479
10,8563 4,3429
4,4004 6,4885
6,6226 12,4081
12,2739 7,5805
7,6571 7,9636
8,0402 10,7414
10,7414 4,2471
4,2854 6,6992
6,7184 12,1973
12,1015 7,6954
7,7337 7,9828
8,0019 10,7605
10,8563 4,2535
6,7535 12,1654
7,7146 7,9923
10,8339
111 Analisis keragaman pengaruh pelarut terhadap kandungan florotanin
Descriptives
Florotanin N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Minimum
Maximum Lower Bound
Upper Bound 1
6 4.253500E0
.1140916 .0465777
4.133768 4.373232
4.1130 4.4004
2 6
6.753500E0 .2117435
.0864439 6.531289
6.975711 6.4885
7.0824 3
6 1.216537E1
.1703759 .0695557
11.986568 12.344165
11.9099 12.4081
4 6
7.714550E0 .0898215
.0366695 7.620288
7.808812 7.5805
7.8103 5
6 7.992333E0
.0290391 .0118552
7.961859 8.022808
7.9636 8.0402
6 6
1.083397E1 .1177281
.0480623 10.710419
10.957515 10.7414
11.0479 Total
36 8.285536E0
2.6399806 .4399968
7.392295 9.178777
4.1130 12.4081
ANOVA
Florotanin Sum of Squares
df Mean Square
F Sig.
Between Groups 243.384
5 48.677 2.664E3
.000 Within Groups
.548 30
.018 Total
243.932 35
112 Post Hoc Tests
Multiple Comparisons
Florotanin LSD
I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Etanol
Metanol -2.5000000
.0780496 .000
-2.714636 -2.285364
Aseton 70 -7.9118667
.0780496 .000
-8.126503 -7.697231
Etanol 80 -3.4610500
.0780496 .000
-3.675686 -3.246414
Metanol 80 -3.7388333
.0780496 .000
-3.953469 -3.524197
Akuades -6.5804667
.0780496 .000
-6.795103 -6.365831
Metanol Etanol
2.5000000 .0780496
.000 2.285364
2.714636 Aseton 70
-5.4118667 .0780496
.000 -5.626503
-5.197231 Etanol 80
-.9610500 .0780496
.000 -1.175686
-.746414 Metanol 80
-1.2388333 .0780496
.000 -1.453469
-1.024197 Akuades
-4.0804667 .0780496
.000 -4.295103
-3.865831 Aseton 70
Etanol 7.9118667
.0780496 .000
7.697231 8.126503
Metanol 5.4118667
.0780496 .000
5.197231 5.626503
Etanol 80 4.4508167
.0780496 .000
4.236181 4.665453
Metanol 80 4.1730333
.0780496 .000
3.958397 4.387669
Akuades 1.3314000
.0780496 .000
1.116764 1.546036
Etanol 80 Etanol
3.4610500 .0780496
.000 3.246414
3.675686 Metanol
.9610500 .0780496
.000 .746414
1.175686 Aseton 70
-4.4508167 .0780496
.000 -4.665453
-4.236181 Metanol 80
-.2777833 .0780496
.001 -.492419
-.063147 Akuades
-3.1194167 .0780496
.000 -3.334053
-2.904781 Metanol 80
Etanol 3.7388333
.0780496 .000
3.524197 3.953469
Metanol 1.2388333
.0780496 .000
1.024197 1.453469
Aseton 70 -4.1730333
.0780496 .000
-4.387669 -3.958397
Etanol 80 .2777833
.0780496 .001
.063147 .492419
Akuades -2.8416333
.0780496 .000
-3.056269 -2.626997
Akuades Etanol
6.5804667 .0780496
.000 6.365831
6.795103 Metanol
4.0804667 .0780496
.000 3.865831
4.295103 Aseton 70
-1.3314000 .0780496
.000 -1.546036
-1.116764 Etanol 80
3.1194167 .0780496
.000 2.904781
3.334053 Metanol 80
2.8416333 .0780496
.000 2.626997
3.056269 . The mean difference is significant at the 0.01 level.
113 Lampiran 4 Aktivitas antioksidan
Data serapan reduksi DPPH menit ke- oleh ekstrak S. echinocarpum
Dosis ppm
Ekstrak I
II III
30 30
30
1000 Etanol
0,265 0,255
0,160 0,170
0,260 0,255
0,165 0,169
0,265 0,261
0,168 0,160
Metanol 1,200
1,300 0,405
0,400 1,400
1,300 0,400
0,400 1,200
1,000 0,400
0,365 Aseton 70
0,520 0,525
0,380 0,380
0,530 0,525
0,380 0,385
0,520 0,520
0,378 0,380
Etanol 80 1,800
1,800 1,300
1,350 1,760
1,760 1,300
1,350 1,800
1,800 1,300
1,280 Metanol 80
0,140 0,235
0,065 0,145
0,140 0,200
0,056 0,150
0,140 0,240
0,065 0,150
Akuades 0,490
0,485 0,395
0,385 0,490
0,480 0,395
0,385 0,490
0,490 0,385
0,385 Tokoferol
0,520 0,505
0,340 0,350
0,590 0,560
0,340 0,360
0,550 0,530
0,340 0,320
500 Etanol
0,320 0,320
0,235 0,215
0,320 0,320
0,240 0,230
0,320 0,320
0,226 0,228
Metanol 1,200
1,150 0,505
0,461 1,150
1,200 0,460
0,440 1.200
1.150 0.498
0.500 Aseton 70
0,640 0,520
0,458 0,472
0,600 0,560
0,472 0,462
0,620 0,540
0,472 0,470
Etanol 80 1,600
1,560 1,320
1,280 1,560
1,600 1,400
1,200 1,600
1,560 1,300
1,240 Metanol 80
0,220 0,200
0,140 0,130
0,220 0,200
0,140 0,150
0,220 0,200
0,136 0,134
Akuades 0,620
0,600 0,546
0,526 0,620
0,600 0,539
0,539 0,620
0,600 0,540
0,524 Tokoferol
0,490 0,470
0,350 0,370
0,490 0,470
0,336 0,332
0,482 0,478
0,340 0,330
200 Etanol
0,315 0,320
0,240 0,224
0,316 0,320
0,252 0,244
0,315 0,321
0,240 0,236
Metanol 1,320
1,260 0,605
0,561 1,300
1,280 0,560
0,552 1,280
1,300 0,591
0,601 Aseton 70
0,680 0,620
0,530 0,555
0,660 0,640
0,549 0,547
0,660 0,640
0,547 0,543
Etanol 80 1,500
1,520 1,313
1,280 1,520
1,500 1,295
1,299 1,500
1,520 1,280
1,272 Metanol 80
0,620 0,580
0,410 0,410
0,620 0,580
0,450 0,430
0,600 0,600
0,420 0,400
Akuades 0,470
0,450 0,430
0,414 0,470
0,450 0,426
0,422 0,460
0,460 0,430
0,410 Tokoferol
0,560 0,550
0,410 0,460
0,560 0,550
0,400 0,410
0,560 0,550
0,402 0,408
100 Etanol
0,245 0,250
0,180 0,185
0,246 0,250
0,198 0,194
0,246 0,250
0,190 0,186
Metanol 1,200
1,300 0,620
0,550 1,200
1,300 0,558
0,554 1,300
1,200 0,600
0,580 Aseton 70
0,520 0,510
0,438 0,432
0,510 0,520
0,439 0,439
0,520 0,510
0,433 0,439
Etanol 80 0,600
0,600 0,530
0,510 0,620
0,580 0,522
0,522 0,620
0,580 0,516
0,508 Metanol 80
0,235 0,220
0,160 0,158
0,226 0,230
0,166 0,172
0,230 0,226
0,160 0,154
Akuades 0,505
0,510 0,481
0,461 0,510
0,506 0,477
0,469 0,507
0,509 0,467
0,469 Tokoferol
0,550 0,530
0,410 0,448
0,540 0,540
0,406 0,396
0,540 0,540
0,404 0,400
114
Data IC
50
Ekstrak
ppm ekstrak S. echinocarpum
I II
III
Etanol 1122
1343 1147
Metanol 186,9
161,2 201,2
Aseton 70 2069
2103 2137
Etanol 80 2310
2439 2050
Metanol 80 781,6
1035 787,3
Akuades 3616
3764 3347
Tokoferol 1481
1048 1059
Data AAST mg100 mg ekstrak S. echinocarpum
Ekstrak I
II III
Etanol 131,9964
78,0342 92,3278
Metanol 792,4024
650,1241 526,3419
Aseton 70 71,5805
49,8336 49,5554
Etanol 80 64,1126
42,9684 51,6585
Metanol 80 189,4831
101,2560 134,5104
Akuades 40,9569
27,8427 31,6403
Analisis keragaman aktivitas antioksidan ekstrak S. echinocarpum
Descriptives
Ekstrak N
Mean Std. Deviation
Std. Error 95 Confidence Interval for Mean
Min Max
Lower Bound Upper Bound
1 3
1.007862E2 27.9577739
1.6141428E1 31.335205
170.237127 78.0343 131.9964
2 3
6.562895E2 133.1373122
7.6866863E1 325.558039
987.020875 526.3419 792.4024 3
3 5.698983E1
12.6366314 7.2957625E0
25.598699 88.380965
49.5555 71.5805
4 3
5.291317E1 10.6277507
6.1359347E0 26.512377
79.313970 42.9684
64.1126 5
3 1.417498E2
44.5568365 2.5724902E1
31.064516 252.435152 101.2560 189.4831
6 3
3.347995E1 6.7478486
3.8958722E0 16.717366
50.242536 27.8427
40.9569 Total
18 1.737014E2
230.4634642 5.4320759E1
59.094618 288.308187
27.8427 792.4024
ANOVA
Ekstrak Sum of Squares
df Mean Square
F Sig.
Between Groups 861306.622
5 172261.324 49.665
.000 Within Groups
41621.319 12
3468.443 Total
902927.942 17
115 Post Hoc Tests
Multiple Comparisons
Ekstrak LSD
I AAST
J AAST
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Etanol
Metanol -5.5550329E2
4.8086334E1 .000
-702.384902 -408.621680
Aseton 70 43.7963340
4.8086334E1 .380
-103.085277 190.677945
Etanol 80 47.8729926
4.8086334E1 .339
-99.008618 194.754604
Metanol 80 -40.9636679
4.8086334E1 .411
-187.845279 105.917943
Akuades 67.3062148
4.8086334E1 .187
-79.575396 214.187826
Metanol Etanol
555.5032912 4.8086334E1
.000 408.621680
702.384902 Aseton 70
599.2996252 4.8086334E1
.000 452.418014
746.181236 Etanol 80
603.3762839 4.8086334E1
.000 456.494673
750.257895 Metanol 80
514.5396233 4.8086334E1
.000 367.658012
661.421234 Akuades
622.8095061 4.8086334E1
.000 475.927895
769.691117 Aseton 70
Etanol -43.7963340
4.8086334E1 .380
-190.677945 103.085277
Metanol -5.9929963E2
4.8086334E1 .000
-746.181236 -452.418014
Etanol 80 4.0766586
4.8086334E1 .934
-142.804952 150.958270
Metanol 80 -84.7600019
4.8086334E1 .103
-231.641613 62.121609
Akuades 23.5098808
4.8086334E1 .634
-123.371730 170.391492
Etanol 80 Etanol
-47.8729926 4.8086334E1
.339 -194.754604
99.008618 Metanol
-6.0337628E2 4.8086334E1
.000 -750.257895
-456.494673 Aseton 70
-4.0766586 4.8086334E1
.934 -150.958270
142.804952 Metanol 80
-88.8366605 4.8086334E1
.089 -235.718272
58.044951 Akuades
19.4332222 4.8086334E1
.693 -127.448389
166.314833 Metanol 80
Etanol 40.9636679
4.8086334E1 .411
-105.917943 187.845279
Metanol -5.1453962E2
4.8086334E1 .000
-661.421234 -367.658012
Aseton 70 84.7600019
4.8086334E1 .103
-62.121609 231.641613
Etanol 80 88.8366605
4.8086334E1 .089
-58.044951 235.718272
Akuades 108.2698827
4.8086334E1 .044
-38.611728 255.151494
Akuades Etanol
-67.3062148 4.8086334E1
.187 -214.187826
79.575396 Metanol
-6.2280951E2 4.8086334E1
.000 -769.691117
-475.927895 Aseton 70
-23.5098808 4.8086334E1
.634 -170.391492
123.371730 Etanol 80
-19.4332222 4.8086334E1
.693 -166.314833
127.448389 Metanol 80
-108.2698827 4.8086334E1
.044 -255.151494
38.611728 . The mean difference is significant at the 0.01 level.
116 Lampiran 5 Keterangan kelaikan etik penelitian
117 Lampiran 6 Berat badan uji toksisitas
Data pengamatan berat badan gram mencit BALBc selama 14 hari
Dosis Jenis
kelamin Ulangan
Hari ke-
1 2
3 4
5 6
7 8
9 10
11 12
13 14
mgkg Jantan
1 19,23
19,34 19,51
19,72 19,88
20,02 20,26
20,54 20,88
21,05 21,35
21,57 21,69
21,87 2
19,03 19,24
19,35 19,46
19,68 19,88
20,08 20,22
20,33 20,48
20,52 20,60
20,67 20,92
3 18,23
18,64 18,80
18,91 19,14
19,71 19,87
20,18 20,28
20,29 20,30
20,89 21,10
21,39 Betina
1 22,30
22,94 23,24
23,57 23,83
24,13 24,33
24,63 24,82
25,15 25,35
25,51 25,71
25,89 2
26,52 26,68
26,97 27,29
27,51 27,88
28,11 28,33
28,67 28,98
29,13 29,37
29,51 29,72
3 20,15
21,23 21,54
21,81 22,11
22,34 22,59
22,83 23,12
23,25 23,42
23,61 23,78
23,92 625
mgkg Jantan
1 23,63
23,65 23,71
23,82 24,14
24,27 24,39
24,56 24,71
24,91 25,15
25,33 25,54
25,77 2
24,57 24,78
25,08 25,15
25,27 25,37
25,47 25,65
25,78 25,88
26,12 26,25
26,41 26,52
3 22,32
22,53 22,63
23,31 23,36
23,56 23,77
23,81 24,01
24,28 24,53
24,62 24,93
25,27 Betina
1 22,94
23,06 23,17
23,28 23,48
23,71 23,88
24,12 24,36
24,55 24,79
25,01 25,29
25,59 2
17,53 17,66
17,81 18,01
18,15 18,27
18,49 18,68
18,86 19,16
19,38 19,52
19,71 19,87
3 25,14
25,19 25,27
25,43 25,58
25,72 25,85
25,97 26,19
26,37 26,64
26,96 27,22
27,55 1250
mgkg Jantan
1 20,86
20,66 20,69
20,76 20,94
21,12 21,21
21,49 21,64
21,78 21,93
22,17 22,35
22,47 2
23,29 23,09
23,31 23,43
23,51 23,65
23,81 23,92
24,07 24,15
24,27 24,39
24,48 24,56
3 25,39
24,89 24,92
25,09 25,23
25,35 25,48
25,56 25,67
25,75 25,87
25,99 26,18
26,35 Betina
1 22,91
22,87 23,01
23,08 23,12
23,16 23,23
23,39 23,51
23,62 23,75
23,82 23,98
24,11 2
22,27 22,15
22,37 22,57
22,91 23,07
23,21 23,38
23,59 23,71
23,87 24,05
24,35 24,58
3 22,28
22,07 22,16
22,45 22,63
22,82 23,02
23,22 23,49
23,65 23,85
24,09 24,23
24,43 2500
mgkg Jantan
1 24,87
24,53 24,34
24,19 24,02
24,08 24,11
24,19 24,25
24,31 24,54
24,72 24,89
25,19 2
28,31 28,23
28,16 27,94
28,07 28,09
28,17 28,26
28,34 28,41
28,51 28,63
28,79 28,89
3 27,83
27,76 27,57
27,45 27,54
27,63 27,68
27,74 27,79
27,88 27,94
28,05 28,11
28,26 Betina
1 24,09
23,87 23,93
24,01 24,17
24,36 24,54
23,71 23,85
24,09 24,19
24,26 24,31
24,49 2
28,05 27,98
28,02 28,14
28,25 28,31
28,38 28,46
28,52 28,66
28,85 28,91
29,06 29,17
3 23,59
23,48 23,38
23,36 23,38
23,43 23,48
23,52 23,59
23,61 23,64
23,67 23,71
23,76 5000
mgkg Jantan
1 27,30
27,18 26,98
26,78 26,64
26,61 26,67
26,86 26,93
27,03 27,14
27,21 27,28
27,35 2
27,63 27,46
27,34 27,17
27,05 27,13
27,22 27,32
27,36 27,47
27,54 27,63
27,69 27,78
3 25,80
25,54 25,31
25,11 24,98
24,93 25,04
25,13 25,16
25,22 25,29
25,48 25,61
25,92 Betina
1 25,00
24,82 24,56
24,36 24,16
23,98 23,71
24,04 24,54
24,65 24,87
24,97 25,07
25,19 2
22,14 21,79
21,65 21,67
21,67 21,67
21,85 21,89
21,94 22,01
22,14 22,28
22,31 22,41
3 25,57
25,47 25,40
25,36 25,28
25,17 25,35
25,55 26,02
26,10 26,16
25,58 25,67
25,73
Rerata data pengamatan berat badan mencit
Dosis Hari ke-
1 2
3 4
5 6
7 8
9 10
11 12
13 14
0 mgkg 20,91
21,35 21,57
21,79 22,03
22,33 22,54
22,79 23,02
23,20 23,35
23,59 23,74
23,95
625 mgkg 22,69
22,81 22,95
23,17 23,33
23,48 23,64
23,79 23,99
24,19 24,44
24,62 24,85
25,09
1250 mgkg 22,83
22,62 22,74
22,89 23,06
23,19 23,33
23,49 23,66
23,78 23,92
24,09 24,26
24,42
2500 mgkg 26,12
25,98 25,90
25,85 25,91
25,98 26,06
25,98 26,06
26,16 26,28
26,37 26,48
26,63
5000 mgkg 25,57
25,34 25,09
24,96 24,49
24,29 24,29
25,13 25,42
25,91 26,22
26,46 26,64
26,95
Analisis keragaman kenaikan berat badan mencit pada akhir masa uji
Descriptives
BB N
Mean Std.
Deviation Std.
Error 95 Confidence Interval for Mean
Min Max
Lower Bound Upper Bound
0 mgkg BB 6 14.64483
3.33377 1.36100 11.146249
18.143417 9.9317 18.7097
625 mgkg BB 6 10.78273
2.26331 .92399
8.407529 13.157930 7.9365
13.3485 1250 mgkg BB
6 7.03543
2.63687 1.07649 4.268210
9.802663 3.7810 10.3727
2500 mgkg BB 6
1.87574 1.12686
.46003 .693180
3.058315 .7206 3.9929
5000 mgkg BB 6
.63273 .34607
.14128 .269547
.995919 .1832 1.2195
Total 30
6.99429 5.75421 1.05057
4.845637 9.142955 .1832
18.7097
ANOVA BB
Sum of Squares df
Mean Square F
Sig. Between Groups
837.322 4
209.330 42.583
.000 Within Groups
122.897 25
4.916 Total
960.219 29
118 Post Hoc Tests
Multiple Comparisons
BB LSD
I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound 0 mgkg BB
625 mgkg BB 3.8621039
1.2800866E0 .006
.293945 7.430263
1250 mgkg BB 7.6093967
1.2800866E0 .000
4.041238 11.177556
2500 mgkg BB 12.7690853
1.2800866E0 .000
9.200926 16.337244
5000 mgkg BB 14.0120996
1.2800866E0 .000
10.443941 17.580259
625 mgkg BB 0 mgkg BB
-3.8621039 1.2800866E0
.006 -7.430263
-.293945 1250 mgkg BB
3.7472929 1.2800866E0
.007 .179134
7.315452 2500 mgkg BB
8.9069814 1.2800866E0
.000 5.338822
12.475141 5000 mgkg BB
10.1499958 1.2800866E0
.000 6.581837
13.718155 1250 mgkg BB
0 mgkg BB -7.6093967
1.2800866E0 .000
-11.177556 -4.041238
625 mgkg BB -3.7472929
1.2800866E0 .007
-7.315452 -.179134
2500 mgkg BB 5.1596885
1.2800866E0 .000
1.591529 8.727848
5000 mgkg BB 6.4027029
1.2800866E0 .000
2.834544 9.970862
2500 mgkg BB 0 mgkg BB
-12.7690853 1.2800866E0
.000 -16.337244
-9.200926 625 mgkg BB
-8.9069814 1.2800866E0
.000 -12.475141
-5.338822 1250 mgkg BB
-5.1596885 1.2800866E0
.000 -8.727848
-1.591529 5000 mgkg BB
1.2430144 1.2800866E0
.341 -2.325145
4.811173 5000 mgkg BB
0 mgkg BB -14.0120996
1.2800866E0 .000
-17.580259 -10.443941
625 mgkg BB -10.1499958
1.2800866E0 .000
-13.718155 -6.581837
1250 mgkg BB -6.4027029
1.2800866E0 .000
-9.970862 -2.834544
2500 mgkg BB -1.2430144
1.2800866E0 .341
-4.811173 2.325145
. The mean difference is significant at the 0.01 level.
119 Lampiran 7 Histopatologis hati dan ginjal
Data pengamatan skor histologis hati
Perlakuan 1
2 3
4 5
6 0 mgkg BB
1 1
0,5 0,5
0,5 625 mgkg BB
1 1,5
1,5 1
1 1250 mgkg BB
1,5 2
1,5 1,5
1,5 1
2500 mgkg BB 2,5
2 2,5
1,67 2
1,67 5000 mgkg BB
2 2
2,33 2
2 2,33
Descriptives
Hati N
Mean Std.
Deviation Std.
Error 95 Confidence Interval for Mean
Minimum Maximum
Lower Bound Upper Bound
0 mgkg BB 6
.5833 .37639 .15366
.1883 .9783
.00 1.00
625 mgkg BB 6
1.0000 .54772 .22361
.4252 1.5748
.00 1.50
1250 mgkg BB 6
1.5000 .31623 .12910
1.1681 1.8319
1.00 2.00
2500 mgkg BB 6
2.0556 .37514 .15315
1.6619 2.4493
1.67 2.50
5000 mgkg BB 6
2.1111 .17212 .07027
1.9305 2.2917
2.00 2.33
Total 30
1.4500 .69776 .12739
1.1895 1.7105
.00 2.50
ANOVA
Hati Sum of Squares
df Mean Square
F Sig.
Between Groups 10.559
4 2.640 18.537
.000 Within Groups
3.560 25
.142 Total
14.119 29
120 Post Hoc Tests
Multiple Comparisons
Hati LSD
I Dosis
J Dosis
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound 0 mgkg BB
625 mgkg BB -.41667
.21787 .067
-1.0240 .1906
1250 mgkg BB -.91667
.21787 .000
-1.5240 -.3094
2500 mgkg BB -1.47223
.21787 .000
-2.0795 -.8649
5000 mgkg BB -1.52777
.21787 .000
-2.1351 -.9205
625 mgkg BB 0 mgkg BB
.41667 .21787
.067 -.1906
1.0240 1250 mgkg BB
-.50000 .21787
.030 -1.1073
.1073 2500 mgkg BB
-1.05557 .21787
.000 -1.6629
-.4483 5000 mgkg BB
-1.11110 .21787
.000 -1.7184
-.5038 1250 mgkg BB
0 mgkg BB .91667
.21787 .000
.3094 1.5240
625 mgkg BB .50000
.21787 .030
-.1073 1.1073
2500 mgkg BB -.55557
.21787 .017
-1.1629 .0517
5000 mgkg BB -.61110
.21787 .010
-1.2184 -.0038
2500 mgkg BB 0 mgkg BB
1.47223 .21787
.000 .8649
2.0795 625 mgkg BB
1.05557 .21787
.000 .4483
1.6629 1250 mgkg BB
.55557 .21787
.017 -.0517
1.1629 5000 mgkg BB
-.05553 .21787
.801 -.6628
.5518 5000 mgkg BB
0 mgkg BB 1.52777
.21787 .000
.9205 2.1351
625 mgkg BB 1.11110
.21787 .000
.5038 1.7184
1250 mgkg BB .61110
.21787 .010
.0038 1.2184
2500 mgkg BB .05553
.21787 .801
-.5518 .6628
. The mean difference is significant at the 0.01 level.
121 Data pengamatan skor histologis ginjal
Perlakuan 1
2 3
4 5
6 0 mgkg BB
0,4 0,2
0,2 0,4
0,2 0,4
625 mgkg BB 0,4
0,6 0,6
0,6 0,4
0,4 1250 mgkg BB
1,6 1,4
1,6 1,6
2 1,4
2500 mgkg BB 1,8
2,2 2
2 2
2,2 5000 mgkg BB
2,6 2,4
2,4 2,6
2,2 2,4
Descriptives
Ginjal N
Mean Std.
Deviation Std. Error 95 Confidence Interval for Mean
Minimum Maximum Lower Bound
Upper Bound 0 mgkg BB
6 .3000
.10954 .04472
.1850 .4150
.20 .40
625 mgkg BB 6
.5333 .10328
.04216 .4249
.6417 .40
.60 1250 mgkg BB
6 1.6000
.21909 .08944
1.3701 1.8299
1.40 2.00
2500 mgkg BB 6
2.0333 .15055
.06146 1.8753
2.1913 1.80
2.20 5000 mgkg BB
6 2.4333
.15055 .06146
2.2753 2.5913
2.20 2.60
Total 30
1.3800 .85879
.15679 1.0593
1.7007 .20
2.60
ANOVA
Ginjal Sum of Squares
df Mean Square
F Sig.
Between Groups 20.808
4 5.202
224.224 .000
Within Groups .580
25 .023
Total 21.388
29
122 Post Hoc Tests
Multiple Comparisons
Ginjal LSD
I Dosis
J Dosis
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound 0 mgkg BB
625 mgkg BB -.23333
.08794 .014
-.4785 .0118
1250 mgkg BB -1.30000
.08794 .000
-1.5451 -1.0549
2500 mgkg BB -1.73333
.08794 .000
-1.9785 -1.4882
5000 mgkg BB -2.13333
.08794 .000
-2.3785 -1.8882
625 mgkg BB 0 mgkg BB
.23333 .08794
.014 -.0118
.4785 1250 mgkg BB
-1.06667 .08794
.000 -1.3118
-.8215 2500 mgkg BB
-1.50000 .08794
.000 -1.7451
-1.2549 5000 mgkg BB
-1.90000 .08794
.000 -2.1451
-1.6549 1250 mgkg BB
0 mgkg BB 1.30000
.08794 .000
1.0549 1.5451
625 mgkg BB 1.06667
.08794 .000
.8215 1.3118
2500 mgkg BB -.43333
.08794 .000
-.6785 -.1882
5000 mgkg BB -.83333
.08794 .000
-1.0785 -.5882
2500 mgkg BB 0 mgkg BB
1.73333 .08794
.000 1.4882
1.9785 625 mgkg BB
1.50000 .08794
.000 1.2549
1.7451 1250 mgkg BB
.43333 .08794
.000 .1882
.6785 5000 mgkg BB
-.40000 .08794
.000 -.6451
-.1549 5000 mgkg BB
0 mgkg BB 2.13333
.08794 .000
1.8882 2.3785
625 mgkg BB 1.90000
.08794 .000
1.6549 2.1451
1250 mgkg BB .83333
.08794 .000
.5882 1.0785
2500 mgkg BB .40000
.08794 .000
.1549 .6451
. The mean difference is significant at the 0.01 level.
123 Lampiran 8 Berat badan uji diabetes
Data pengamatan berat badan tikus percobaan selama masa penelitian
Perlk. 2
4 6
8 10
12
Normal-1 125,65
139,66 153,82
169,44 185,02
202,15 218,25
Normal-2 124,74
139,04 155,12
172,32 186,82
204,65 219,56
Normal-3 122,25
137,64 153,12
170,24 185,08
201,74 217,56
Normal-4 127,65
141,98 155,86
169,24 184,26
199,82 220,12
Normal-5 126,98
141,31 157,04
171,68 186,48
203,12 218,48
Normal-6 128,12
142,86 157,91
171,32 186,26
200,12 217,28
DM + S0-1 152,12
147,56 142,15
135,64 122,68
110,62 100,86
DM + S0-2 148,65
144,64 140,22
134,65 121,82
112,12 101,14
DM + S0-3 147,98
143,84 139,24
132,13 121,32
111,89 100,84
DM + S0-4 149,65
145,54 140,06
134,68 120,56
112,45 101,58
DM + S0-5 149,55
145,88 139,78
132,48 120,36
- -
DM + S0-6 151,86
146,78 140,34
133,42 -
- -
DM + S150-1 142,52
137,64 133,23
128,78 123,56
116,68 108,25
DM + S150-2 140,38
135,68 131,28
127,68 121,24
114,48 105,68
DM + S150-3 139,25
137,14 133,12
128,86 122,46
115,64 106,98
DM + S150-4 141,45
137,26 132,82
127,98 121,32
114,84 106,12
DM + S150-5 143,24
138,62 134,26
130,02 123,45
115,12 -
DM + S150-6 140,12
135,84 132,84
128,28 122,48
- -
DM + S300-1 135,64
132,28 128,78
125,14 121,25
117,65 114,98
DM + S300-2 138,42
135,88 132,82
128,86 123,64
118,45 115,82
DM + S300-3 139,62
137,06 134,64
128,98 125,32
120,84 117,46
DM + S300-4 136,27
133,48 130,84
126,82 122,42
118,62 115,64
DM + S300-5 137,02
134,62 131,82
127,56 123,68
119,86 -
DM + S300-6 135,12
132,36 129,78
125,68 121,46
117,78 -
DM + S450-1 130,44
128,86 126,62
124,68 125,02
127,32 130,85
DM + S450-2 129,78
128,14 125,82
124,54 123,56
123,46 124,02
DM + S450-3 128,88
127,32 125,78
124,78 125,88
126,92 129,32
DM + S450-4 130,48
128,46 126,28
125,68 124,12
124,62 125,14
DM + S450-5 130,25
128,12 126,34
125,32 123,64
124,08 125,68
DM + S450-6 130,24
127,86 126,02
125,84 123,34
124,22 125,67
Rerata berat badan tikus percobaan selama masa penelitian
Perlk. 2
4 6
8 10
12
Normal 125,8983
140,4150 155,4783
177,0670 185,6533
210,9333 218,5417
DM + S0 149,9683
145,7067 140,2983
133,8333 121,3480
111,7700 101,1500
DM + S150 141,1600
137,0300 132,9250
128,6000 122,4183
115,3520 106,7575
DM + S300 137,0150
134,2800 131,4467
127,1733 122,9617
118,8667 115,9750
DM + S450 130,0117
128,1267 126,1433
125,1400 124,2600
125,1033 126,7800
124 Analisis keragaman berat badan g tikus percobaan
Perlk. 1
2 3
4 5
6
Normal 218,25
219,56 217,56
220,12 218,48
217,28 DM + S0
100,86 101,14
100,84 101,58
- -
DM + S150 108,25
105,68 106,98
106,12 -
- DM + S300
114,98 115,82
117,46 115,64
- -
DM + S450 130,85
124,02 129,32
125,14 125,68
125,67
Descriptives
BB N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
6 2.1854E2
1.11108 .45360
217.3757 219.7077 217.28 220.12
DM + S0 4
1.0110E2 .34501
.17251 100.5560
101.6540 100.84 101.58 DM + S150
4 1.0676E2
1.13203 .56601
104.9562 108.5588 105.68 108.25
DM + S300 4
1.1998E2 4.34709
2.17354 113.0578
126.8922 115.82 125.64 DM + S450
6 1.3940E2
1.64694 .67236
137.6683 141.1250 136.94 141.07
Total 24
1.4412E2 46.04483
9.39886 124.6811
163.5672 100.84 220.12
ANOVA BB
Sum of Squares df
Mean Square F
Sig. Between Groups
48682.276 4
12170.569 2.868E3
.000 Within Groups
80.628 19
4.244 Total
48762.904 23
125 Post Hoc Tests
Multiple Comparisons
BB LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
117.43667 1.32972
.000 113.6324
121.2409 DM + S150
111.78417 1.32972
.000 107.9799
115.5884 DM + S300
98.56667 1.32972
.000 94.7624
102.3709 DM + S450
79.14500 1.18934
.000 75.7424
82.5476 DM + S0
Normal -117.43667
1.32972 .000
-121.2409 -113.6324
DM + S150 -5.65250
1.45663 .001
-9.8198 -1.4852
DM + S300 -18.87000
1.45663 .000
-23.0373 -14.7027
DM + S450 -38.29167
1.32972 .000
-42.0959 -34.4874
DM + S150 Normal -111.78417
1.32972 .000
-115.5884 -107.9799
DM + S0 5.65250
1.45663 .001
1.4852 9.8198
DM + S300 -13.21750
1.45663 .000
-17.3848 -9.0502
DM + S450 -32.63917
1.32972 .000
-36.4434 -28.8349
DM + S300 Normal -98.56667
1.32972 .000
-102.3709 -94.7624
DM + S0 18.87000
1.45663 .000
14.7027 23.0373
DM + S150 13.21750
1.45663 .000
9.0502 17.3848
DM + S450 -19.42167
1.32972 .000
-23.2259 -15.6174
DM + S450 Normal -79.14500
1.18934 .000
-82.5476 -75.7424
DM + S0 38.29167
1.32972 .000
34.4874 42.0959
DM + S150 32.63917
1.32972 .000
28.8349 36.4434
DM + S300 19.42167
1.32972 .000
15.6174 23.2259
. The mean difference is significant at the 0.01 level.
126 Lampiran 9 Glukosa darah
Data pengamatan glukosa darah tikus percobaan selama masa penelitian
Perlakuan 2
4 6
8 10
12
1 102
98 106
110 101
103 105
2 110
97 101
102 115
98 102
Normal 3
93 105
99 94
104 112
104 4
105 97
106 102
99 106
99 5
94 112
116 99
99 103
117 6
97 100
95 94
100 99
110 1
467 447
401 432
378 465
412 2
498 535
441 449
412 456
446 DM + S0
3 325
421 325
368 340
352 432
4 542
356 439
361 346
419 452
5 512
521 486
479 486
- -
6 562
545 512
541 -
- -
1 501
542 432
461 402
378 398
2 521
468 421
432 376
398 381
DM + S150 3
465 478
492 412
403 386
356 4
514 465
478 368
414 364
378 5
496 455
389 376
358 387
- 6
541 478
456 412
421 -
- 1
465 492
412 432
365 341
332 2
478 451
364 322
314 331
278 DM + S300
3 489
464 421
442 378
312 304
4 513
475 397
387 332
354 332
5 505
512 465
434 427
414 -
6 521
487 468
416 462
416 -
1 404
565 335
411 464
234 206
2 575
461 375
300 214
248 214
DM + S450 3
507 537
340 360
254 214
235 4
515 361
312 189
201 183
174 5
381 370
305 274
216 198
186 6
361 314
311 314
283 180
161
Analisis keragaman glukosa darah tikus percobaan
Descriptives
Glukosa N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
6 106.17
6.432 2.626
99.42 112.92
99 117
DM + S0 4
435.50 17.767
8.884 407.23
463.77 412
452 DM + S150
4 378.25
17.251 8.625
350.80 405.70
356 398
DM + S300 4
311.50 25.942
12.971 270.22
352.78 278
332 DM + S450
6 196.00
27.401 11.186
167.24 224.76
161 235
Total 24
263.08 125.784
25.676 209.97
316.20 99
452
ANOVA
Glukosa Sum of Squares
df Mean Square
F Sig.
Between Groups 356078.250
4 89019.562
216.299 .000
Within Groups 7819.583
19 411.557
Total 363897.833
23
127 Post Hoc Tests
Multiple Comparisons
Glukosa LSD
I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Normal
DM + S0 -329.333
13.095 .000
-366.80 -291.87
DM + S150 -272.083
13.095 .000
-309.55 -234.62
DM + S300 -205.333
13.095 .000
-242.80 -167.87
DM + S450 -89.833
11.713 .000
-123.34 -56.32
DM + S0 Normal
329.333 13.095
.000 291.87
366.80 DM + S150
57.250 14.345
.001 16.21
98.29 DM + S300
124.000 14.345
.000 82.96
165.04 DM + S450
239.500 13.095
.000 202.04
276.96 DM + S150 Normal
272.083 13.095
.000 234.62
309.55 DM + S0
-57.250 14.345
.001 -98.29
-16.21 DM + S300
66.750 14.345
.000 25.71
107.79 DM + S450
182.250 13.095
.000 144.79
219.71 DM + S300 Normal
205.333 13.095
.000 167.87
242.80 DM + S0
-124.000 14.345
.000 -165.04
-82.96 DM + S150
-66.750 14.345
.000 -107.79
-25.71 DM + S450
115.500 13.095
.000 78.04
152.96 DM + S450 Normal
89.833 11.713
.000 56.32
123.34 DM + S0
-239.500 13.095
.000 -276.96
-202.04 DM + S150
-182.250 13.095
.000 -219.71
-144.79 DM + S300
-115.500 13.095
.000 -152.96
-78.04 . The mean difference is significant at the 0.01 level.
128 Lampiran 10 Hemoglobin A
1
c
Data pengamatan HbA
1
Perlakuan
c tikus percobaan
1 2
3 4
5 6
Normal 5,2857
5,1812 5,2509
5,0767 5,7038
5,4599 DM + S0
15,9826 17,1673
16,6794 17,3763
- -
DM + S150 15,4948
14,9024 14,0314
14,7979 -
- DM + S300
13,1951 11,3136
12,2195 13,1951
- -
DM + S450 8,8049
9,0836 9,8153
7,6899 8,1080
7,2369
Analisis keragaman HbA
1c
tikus percobaan
Descriptives
HbA
1c
N Mean
Std. Deviation
Std. Error 95 Confidence Interval for Mean
Min Max
Lower Bound Upper Bound
Normal 6
5.153558E0 .1806655 .0737564
4.963961 5.343155
4.9522 5.4579
DM + S0 4
1.440449E1 .4990733 .2495367
13.610357 15.198631
13.7444 14.8680
DM + S150 4
1.279635E1 .4845675 .2422838
12.025293 13.567403
12.1713 13.3511
DM + S300 4
1.092135E1 .7287147 .3643574
9.761801 12.080896
9.9803 11.4972
DM + S450 6
7.676966E0 .7696834 .3142219
6.869233 8.484700
6.6938 8.7725
Total 24
9.561330E0 3.5332618 .7212240
8.069364 11.053295
4.9522 14.8680
ANOVA
HbA
1c
Sum of Squares df
Mean Square F
Sig. Between Groups
280.961 4
70.240 216.299
.000 Within Groups
6.170 19
.325 Total
287.131 23
129 Post Hoc Tests
Multiple Comparisons
HbA
1c
LSD I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Normal
DM + S0 -9.2509363
.3678404 .000
-10.303304 -8.198569
DM + S150 -7.6427903
.3678404 .000
-8.695157 -6.590423
DM + S300 -5.7677903
.3678404 .000
-6.820157 -4.715423
DM + S450 -2.5234082
.3290064 .000
-3.464674 -1.582142
DM + S0 Normal
9.2509363 .3678404
.000 8.198569
10.303304 DM + S150
1.6081461 .4029489
.001 .455336
2.760957 DM + S300
3.4831461 .4029489
.000 2.330336
4.635957 DM + S450
6.7275281 .3678404
.000 5.675161
7.779895 DM + S150 Normal
7.6427903 .3678404
.000 6.590423
8.695157 DM + S0
-1.6081461 .4029489
.001 -2.760957
-.455336 DM + S300
1.8750000 .4029489
.000 .722189
3.027811 DM + S450
5.1193820 .3678404
.000 4.067015
6.171749 DM + S300 Normal
5.7677903 .3678404
.000 4.715423
6.820157 DM + S0
-3.4831461 .4029489
.000 -4.635957
-2.330336 DM + S150
-1.8750000 .4029489
.000 -3.027811
-.722189 DM + S450
3.2443820 .3678404
.000 2.192015
4.296749 DM + S450 Normal
2.5234082 .3290064
.000 1.582142
3.464674 DM + S0
-6.7275281 .3678404
.000 -7.779895
-5.675161 DM + S150
-5.1193820 .3678404
.000 -6.171749
-4.067015 DM + S300
-3.2443820 .3678404
.000 -4.296749
-2.192015 . The mean difference is significant at the 0.01 level.
130 Lampiran 11 Malondialdehida MDA
Data pengamatan serapan serum tikus percobaan
Absorbansi Standar
2,487 2,487
Blanko 0,042
0,042
Absorbansi
Perlakuan I
II rerata
Normal 1
0,095 0,108
0,1015 2
0,115 0,115
0,1150 3
0,139 0,121
0,1300 4
0,109 0,105
0,1070 5
0,119 0,112
0,1155 6
0,099 0,101
0,1000 DM + S0
1 1,795
1,745 1,7700
2 1,698
1,785 1,7415
3 1,587
1,624 1,6055
4 1,702
1,624 1,6630
DM + S150 1
1,117 1,325
1,2210 2
1,202 1,249
1,2255 3
1,098 1,118
1,1080 4
1,008 1,226
1,1170 DM + S300
1 0,664
0,664 0,6640
2 0,712
0,713 0,7125
3 0,712
0,712 0,7120
4 0,655
0,655 0,6550
DM + S450 1
0,251 0,251
0,2510 2
0,299 0,295
0,2970 3
0,254 0,243
0,2485 4
0,291 0,304
0,2975 5
0,291 0,287
0,2890 6
0,311 0,314
0,3125
Data MDA serum tikus percobaan
Perlakuan 1
2 3
4 5
6
Normal 0,2433
0,2986 0,3599
0,2658 0,3006
0,2372 DM + S0
7,0675 6,9509
6,3947 6,6299
- -
DM + S150 4,8221
4,8405 4,3599
4,3967 -
- DM + S300
2,5439 2,7423
2,7403 2,5072
- -
DM + S450 0,8548
1,0429 0,8446
1,0449 1,0102
1,1063
131 Analisis keragaman MDA serum tikus percobaan
Descriptives
MDA N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
6 .284254
.0456541 .0186382 .236342
.332165 .2372
.3599 DM + S0
4 6.760736E0
.3062618 .1531309 6.273405
7.248067 6.3947
7.0675 DM + S150
4 4.604806E0
.2620587 .1310294 4.187812
5.021800 4.3599
4.8405 DM + S300
4 2.633436E0
.1254672 .0627336 2.433789
2.833082 2.5072
2.7423 DM + S450
6 .983981
.1086074 .0443388 .870004
1.097957 .8446
1.1063 Total
24 2.650222E0
2.4111792 .4921799 1.632070
3.668373 .2372
7.0675
ANOVA MDA
Sum of Squares df
Mean Square F
Sig. Between Groups
133.113 4
33.278 1.047E3 .000
Within Groups .604
19 .032
Total 133.717
23
Post Hoc Tests
Multiple Comparisons
MDA LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
-6.4764826 .1150933
.000 -6.805757
-6.147208 DM + S150
-4.3205521 .1150933
.000 -4.649826
-3.991278 DM + S300
-2.3491820 .1150933
.000 -2.678456
-2.019908 DM + S450
-.6997273 .1029426
.000 -.994239
-.405215 DM + S0
Normal 6.4764826
.1150933 .000
6.147208 6.805757
DM + S150 2.1559305
.1260784 .000
1.795229 2.516632
DM + S300 4.1273006
.1260784 .000
3.766599 4.488003
DM + S450 5.7767553
.1150933 .000
5.447481 6.106030
DM + S150 Normal 4.3205521
.1150933 .000
3.991278 4.649826
DM + S0 -2.1559305
.1260784 .000
-2.516632 -1.795229
DM + S300 1.9713701
.1260784 .000
1.610668 2.332072
DM + S450 3.6208248
.1150933 .000
3.291550 3.950099
DM + S300 Normal 2.3491820
.1150933 .000
2.019908 2.678456
DM + S0 -4.1273006
.1260784 .000
-4.488003 -3.766599
DM + S150 -1.9713701
.1260784 .000
-2.332072 -1.610668
DM + S450 1.6494547
.1150933 .000
1.320180 1.978729
DM + S450 Normal .6997273
.1029426 .000
.405215 .994239
DM + S0 -5.7767553
.1150933 .000
-6.106030 -5.447481
DM + S150 -3.6208248
.1150933 .000
-3.950099 -3.291550
DM + S300 -1.6494547
.1150933 .000
-1.978729 -1.320180
. The mean difference is significant at the 0.01 level.
132 Lampiran 12 Aktivitas Superoksida Dismutase SOD
Data pengamatan
Perlakuan 1
2 3
4 Keterangan
Normal 1
0,051 0,051
0,052 0,053
Sampel 0,055
0,055 0,056
0,057 Tanpa sampel
0,014 0,015
0,015 0,016
Blanko 2
0,050 0,050
0,051 0,052
Sampel 0,054
0,054 0,055
0,056 Tanpa sampel
0,014 0,014
0,015 0,016
Blanko 3
0,051 0,052
0,052 0,053
Sampel 0,055
0,056 0,056
0,057 Tanpa sampel
0,013 0,014
0,015 0,016
Blanko 4
0,050 0,050
0,051 0,051
Sampel 0,054
0,054 0,055
0,055 Tanpa sampel
0,013 0,013
0,014 0,014
Blanko 5
0,050 0,050
0,051 0,051
Sampel 0,053
0,054 0,055
0,056 Tanpa sampel
0,015 0,015
0,016 0,016
Blanko 6
0,049 0,050
0,050 0,051
Sampel 0,053
0,054 0,054
0,055 Tanpa sampel
0,015 0,015
0,016 0,016
Blanko
DM + S0 1
0,054 0,054
0,055 0,055
Sampel 0,055
0,055 0,056
0,057 Tanpa sampel
0,015 0,016
0,016 0,016
Blanko 2
0,052 0,053
0,054 0,054
Sampel 0,053
0,054 0,055
0,056 Tanpa sampel
0,014 0,014
0,014 0,015
Blanko 3
0,054 0,054
0,055 0,055
Sampel 0,055
0,056 0,056
0,057 Tanpa sampel
0,014 0,014
0,015 0,016
Blanko 4
0,052 0,052
0,052 0,053
Sampel 0,053
0,053 0,054
0,054 Tanpa sampel
0,016 0,016
0,017 0,017
Blanko
DM + S150 1
0,052 0,052
0,052 0,053
Sampel 0,053
0,054 0,054
0,055 Tanpa sampel
0,014 0,015
0,016 0,016
Blanko 2
0,053 0,053
0,054 0,054
Sampel 0,054
0,055 0,056
0,056 Tanpa sampel
0,016 0,016
0,017 0,018
Blanko 3
0,050 0,051
0,051 0,052
Sampel 0,052
0,052 0,053
0,054 Tanpa sampel
0,012 0,013
0,013 0,014
Blanko 4
0,052 0,052
0,052 0,053
Sampel 0,053
0,054 0,054
0,055 Tanpa sampel
0,016 0,017
0,017 0,017
Blanko
DM + S300 1
0,053 0,053
0,053 0,053
Sampel 0,055
0,055 0,056
0,056 Tanpa sampel
0,015 0,016
0,016 0,016
Blanko 2
0,051 0,051
0,051 0,052
Sampel 0,053
0,053 0,054
0,054 Tanpa sampel
0,014 0,014
0,015 0,015
Blanko 3
0,052 0,052
0,052 0,053
Sampel 0,054
0,054 0,055
0,056 Tanpa sampel
0,013 0,014
0,015 0,016
Blanko 4
0,051 0,052
0,052 0,053
Sampel 0,054
0,054 0,055
0,055 Tanpa sampel
0,012 0,013
0,013 0,014
Blanko
133
DM + S450 1
0,051 0,051
0,052 0,053
Sampel 0,054
0,055 0,055
0,056 Tanpa sampel
0,013 0,014
0,014 0,015
Blanko 2
0,05 0,05
0,051 0,051
Sampel 0,053
0,053 0,054
0,055 Tanpa sampel
0,015 0,015
0,016 0,016
Blanko 3
0,051 0,052
0,052 0,052
Sampel 0,054
0,055 0.055
0,056 Tanpa sampel
0,014 0,014
0,015 0,015
Blanko 4
0,05 0,051
0,051 0,051
Sampel 0,053
0,054 0,054
0,055 Tanpa sampel
0,014 0,015
0,015 0,015
Blanko 5
0,052 0,052
0,052 0,053
Sampel 0,055
0,055 0,056
0,056 Tanpa sampel
0,015 0,016
0,016 0,017
Blanko 6
0,05 0,05
0,051 0,051
Sampel 0,053
0,053 0,054
0,054 Tanpa sampel
0,015 0,015
0,016 0,016
Blanko
Data aktivitas SOD tikus percobaan
Perlakuan 1
2 3
4 5
6
Normal 36,6972
37,6068 43,7247
39,5349 37,7104
38,2605 DM + S0
14,0351 12,5786
11,7647 13,2992
- -
DM + S150 16,4557
18,0904 17,8862
18,8235 -
- DM + S300
25,7757 25,7511
25,9740 23,6842
- -
DM + S450 31,0078
29,4737 32,4324
30,9524 32,5926
31,3073
Analisis keragaman aktivitas SOD tikus percobaan
Descriptives
SOD N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
6 3.892249E1
2.5308307 1.0332073E0
36.266548 41.578436
36.6972 43.7247
DM + S0 4
1.291941E1 .9727090
.4863545 11.371613
14.467207 11.7647
14.0351 DM + S150
4 1.781396E1
.9909208 .4954604
16.237188 19.390740
16.4557 18.8235
DM + S300 4
2.529624E1 1.0793111
.5396555 23.578816
27.013666 23.6842
25.9740 DM + S450
6 3.129436E1
1.1397138 .4652862
30.098303 32.490416
29.4737 32.5926
Total 24
2.689248E1 9.6757027
1.9750445E0 22.806791
30.978173 11.7647
43.7247
ANOVA SOD
Sum of Squares df
Mean Square F
Sig. Between Groups
2105.443 4
526.361 209.226
.000 Within Groups
47.799 19
2.516 Total
2153.242 23
134 Post Hoc Tests
Multiple Comparisons
SOD LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
26.0030820 1.0238307E0
.000 23.073969
28.932195 DM + S150
21.1085280 1.0238307E0
.000 18.179415
24.037641 DM + S300
13.6262510 1.0238307E0
.000 10.697138
16.555364 DM + S450
7.6281327 .9157420
.000 5.008255
10.248011 DM + S0
Normal -26.0030820
1.0238307E0 .000
-28.932195 -23.073969
DM + S150 -4.8945540
1.1215503E0 .000
-8.103236 -1.685872
DM + S300 -12.3768310
1.1215503E0 .000
-15.585513 -9.168149
DM + S450 -18.3749493
1.0238307E0 .000
-21.304062 -15.445837
DM + S150 Normal -21.1085280
1.0238307E0 .000
-24.037641 -18.179415
DM + S0 4.8945540
1.1215503E0 .000
1.685872 8.103236
DM + S300 -7.4822770
1.1215503E0 .000
-10.690959 -4.273595
DM + S450 -13.4803953
1.0238307E0 .000
-16.409508 -10.551283
DM + S300 Normal -13.6262510
1.0238307E0 .000
-16.555364 -10.697138
DM + S0 12.3768310
1.1215503E0 .000
9.168149 15.585513
DM + S150 7.4822770
1.1215503E0 .000
4.273595 10.690959
DM + S450 -5.9981183
1.0238307E0 .000
-8.927231 -3.069006
DM + S450 Normal -7.6281327
.9157420 .000
-10.248011 -5.008255
DM + S0 18.3749493
1.0238307E0 .000
15.445837 21.304062
DM + S150 13.4803953
1.0238307E0 .000
10.551283 16.409508
DM + S300 5.9981183
1.0238307E0 .000
3.069006 8.927231
. The mean difference is significant at the 0.01 level.
135 Lampiran 13 Profil Cu,Zn-SOD ginjal
Data pengamatan
Perlakuan Preparat
+++ ++
+- -
95 152
156 34
I 72
164 144
22 77
134 125
18 88
157 153
25 Normal
II 84
162 166
24 87
166 154
20 82
152 142
22 III
76 147
151 20
89 165
150 21
10 133
245 I
12 125
261 8
126 228
5 110
205 DM + S0
II 7
100 196
4 103
208 5
110 207
III 7
106 204
6 111
219 1
13 109
201 I
15 119
225 13
118 216
12 106
196 DM + S150
II 1
10 105
194 9
101 191
10 116
211 III
11 107
202 1
11 109
209 1
24 128
190 I
1 23
118 180
24 122
183 23
112 181
DM + S300 II
1 22
118 196
1 23
125 176
1 21
124 180
III 24
120 193
1 23
126 173
8 43
233 73
I 9
42 225
68 10
42 234
72 8
38 224
69 DM + S450
II 9
44 237
68 9
40 224
64 9
39 228
70 III
7 40
213 68
8 42
220 65
136
Analisis keragaman profil positif kuat +++ Cu,Zn-SOD ginjal
Descriptives
Profil kuat N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 20.29767 .1603300
.0925666 19.899392
20.695956 20.1482
20.4670 DM + S0
3 .00000
.0000000 .0000000
.000000 .000000
.0000 .0000
DM + S150 3
.10380 .0033555
.0019373 .095470
.112141 .1010
.1075 DM + S300
3 .20254
.0025468 .0014704
.196215 .208869
.2007 .2054
DM + S450 3
2.47981 .0929370
.0536572 2.248943
2.710680 2.3745
2.5502 Total
15 4.61676
8.1719958 2.1100002E0
.091266 9.142267
.0000 20.4670
ANOVA
Profil kuat Sum of Squares
df Mean Square
F Sig.
Between Groups 934.872
4 233.718 3.401E4
.000 Within Groups
.069 10
.007 Total
934.941 14
Post Hoc Tests
Multiple Comparisons
Profil kuat LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
20.2976741 .0676863
.000 20.083158
20.512190 DM + S150
20.1938682 .0676863
.000 19.979352
20.408385 DM + S300
20.0951321 .0676863
.000 19.880616
20.309648 DM + S450
17.8178624 .0676863
.000 17.603346
18.032379 DM + S0
Normal -20.2976741
.0676863 .000
-20.512190 -20.083158
DM + S150 -.1038059
.0676863 .156
-.318322 .110710
DM + S300 -.2025420
.0676863 .014
-.417058 .011974
DM + S450 -2.4798117
.0676863 .000
-2.694328 -2.265295
DM + S150 Normal -20.1938682
.0676863 .000
-20.408385 -19.979352
DM + S0 .1038059
.0676863 .156
-.110710 .318322
DM + S300 -.0987362
.0676863 .175
-.313252 .115780
DM + S450 -2.3760058
.0676863 .000
-2.590522 -2.161489
DM + S300 Normal -20.0951321
.0676863 .000
-20.309648 -19.880616
DM + S0 .2025420
.0676863 .014
-.011974 .417058
DM + S150 .0987362
.0676863 .175
-.115780 .313252
DM + S450 -2.2772697
.0676863 .000
-2.491786 -2.062753
DM + S450 Normal -17.8178624
.0676863 .000
-18.032379 -17.603346
DM + S0 2.4798117
.0676863 .000
2.265295 2.694328
DM + S150 2.3760058
.0676863 .000
2.161489 2.590522
DM + S300 2.2772697
.0676863 .000
2.062753 2.491786
. The mean difference is significant at the 0.01 level.
137
Analisis keragaman profil positif moderat ++ Cu,Zn-SOD ginjal
Descriptives
Profil moderat N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 3.787817E1
.2046262 .1181410
37.369852 38.386491
37.7159 38.1080
DM + S0 3
2.054625E0 .4777562
.2758326 .867813
3.241437 1.7142
2.6008 DM + S150
3 3.523634E0
.3975058 .2295001
2.536175 4.511093
3.2461 3.9790
DM + S300 3
7.000277E0 .1296053
.0748277 6.678319
7.322234 6.8947
7.1449 DM + S450
3 1.192883E1
.1204462 .0695396
11.629623 12.228033
11.7898 12.0014
Total 15
1.247711E1 13.6115194
3.5144792E0 4.939299
20.014915 1.7142
38.1080
ANOVA
Profil moderat Sum of Squares
df Mean Square
F Sig.
Between Groups 2592.910
4 648.227 7.055E3
.000 Within Groups
.919 10
.092 Total
2593.828 14
Post Hoc Tests
Multiple Comparisons
Profil moderat LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
35.8235469 .2475045
.000 35.039138
36.607956 DM + S150
34.3545379 .2475045
.000 33.570129
35.138947 DM + S300
30.8778949 .2475045
.000 30.093486
31.662304 DM + S450
25.9493435 .2475045
.000 25.164934
26.733753 DM + S0
Normal -35.8235469
.2475045 .000
-36.607956 -35.039138
DM + S150 -1.4690090
.2475045 .000
-2.253418 -.684600
DM + S300 -4.9456520
.2475045 .000
-5.730061 -4.161243
DM + S450 -9.8742034
.2475045 .000
-10.658613 -9.089794
DM + S150 Normal -34.3545379
.2475045 .000
-35.138947 -33.570129
DM + S0 1.4690090
.2475045 .000
.684600 2.253418
DM + S300 -3.4766430
.2475045 .000
-4.261052 -2.692234
DM + S450 -8.4051944
.2475045 .000
-9.189604 -7.620785
DM + S300 Normal -30.8778949
.2475045 .000
-31.662304 -30.093486
DM + S0 4.9456520
.2475045 .000
4.161243 5.730061
DM + S150 3.4766430
.2475045 .000
2.692234 4.261052
DM + S450 -4.9285514
.2475045 .000
-5.712961 -4.144142
DM + S450 Normal -25.9493435
.2475045 .000
-26.733753 -25.164934
DM + S0 9.8742034
.2475045 .000
9.089794 10.658613
DM + S150 8.4051944
.2475045 .000
7.620785 9.189604
DM + S300 4.9285514
.2475045 .000
4.144142 5.712961
. The mean difference is significant at the 0.01 level.
138 Analisis keragaman profil positif lemah +- Cu,Zn-SOD ginjal
Descriptives
Profil lemah N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 3.627068E1
.5966189 .3444581
34.788598 37.752765
35.6099 36.7697
DM + S0 3
3.346716E1 .0967600
.0558644 33.226793
33.707523 33.3589
33.5452 DM + S150
3 3.365203E1
.0675511 .0390006
33.484226 33.819838
33.5985 33.7279
DM + S300 3
3.695774E1 .6244898
.3605493 35.406418
38.509056 36.3065
37.5515 DM + S450
3 6.569883E1
.4827689 .2787268
64.499567 66.898097
65.3454 66.2489
Total 15
4.120929E1 12.7608604
3.2948400E0 34.142559
48.276017 33.3589
66.2489
ANOVA
Profil lemah Sum of Squares
df Mean Square
F Sig.
Between Groups 2277.768
4 569.442 2.867E3
.000 Within Groups
1.986 10
.199 Total
2279.754 14
Post Hoc Tests
Multiple Comparisons
Profil lemah LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
2.8035239 .3638558
.000 1.650365
3.956682 DM + S150
2.6186498 .3638558
.000 1.465491
3.771808 DM + S300
-.6870555 .3638558
.088 -1.840214
.466103 DM + S450
-29.4281504 .3638558
.000 -30.581309
-28.274992 DM + S0
Normal -2.8035239
.3638558 .000
-3.956682 -1.650365
DM + S150 -.1848741
.3638558 .622
-1.338032 .968284
DM + S300 -3.4905793
.3638558 .000
-4.643738 -2.337421
DM + S450 -32.2316743
.3638558 .000
-33.384833 -31.078516
DM + S150 Normal -2.6186498
.3638558 .000
-3.771808 -1.465491
DM + S0 .1848741
.3638558 .622
-.968284 1.338032
DM + S300 -3.3057053
.3638558 .000
-4.458864 -2.152547
DM + S450 -32.0468002
.3638558 .000
-33.199959 -30.893642
DM + S300 Normal .6870555
.3638558 .088
-.466103 1.840214
DM + S0 3.4905793
.3638558 .000
2.337421 4.643738
DM + S150 3.3057053
.3638558 .000
2.152547 4.458864
DM + S450 -28.7410949
.3638558 .000
-29.894253 -27.587937
DM + S450 Normal 29.4281504
.3638558 .000
28.274992 30.581309
DM + S0 32.2316743
.3638558 .000
31.078516 33.384833
DM + S150 32.0468002
.3638558 .000
30.893642 33.199959
DM + S300 28.7410949
.3638558 .000
27.587937 29.894253
. The mean difference is significant at the 0.01 level.
139 Analisis keragaman profil negatif - Cu,Zn-SOD ginjal
Descriptives
Profil negatif N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 5.553473E0
.4929044 .2845785
4.329030 6.777915
5.1817 6.1126
DM + S0 3
6.447822E1 .5242984
.3027038 63.175788
65.780647 63.9019 64.9269
DM + S150 3
6.272053E1 .3619066
.2089468 61.821503
63.619554 62.3197 63.0232
DM + S300 3
5.583944E1 .6161714
.3557467 54.308789
57.370099 55.3484 56.5309
DM + S450 3
1.989253E1 .3863269
.2230460 18.932839
20.852217 19.4465 20.1220
Total 15
4.169684E1 25.1149759
6.4846589E0 27.788628
55.605048 5.1817
64.9269
ANOVA
Profil negatif Sum of Squares
df Mean Square
F Sig.
Between Groups 8828.313
4 2207.078 9.370E3
.000 Within Groups
2.355 10
.236 Total
8830.668 14
Post Hoc Tests
Multiple Comparisons
Profil negatif LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
-58.9247449 .3962719
.000 -60.180639
-57.668851 DM + S150
-57.1670559 .3962719
.000 -58.422950
-55.911162 DM + S300
-50.2859716 .3962719
.000 -51.541865
-49.030078 DM + S450
-14.3390555 .3962719
.000 -15.594949
-13.083162 DM + S0
Normal 58.9247449
.3962719 .000
57.668851 60.180639
DM + S150 1.7576890
.3962719 .001
.501795 3.013583
DM + S300 8.6387734
.3962719 .000
7.382880 9.894667
DM + S450 44.5856894
.3962719 .000
43.329796 45.841583
DM + S150 Normal 57.1670559
.3962719 .000
55.911162 58.422950
DM + S0 -1.7576890
.3962719 .001
-3.013583 -.501795
DM + S300 6.8810844
.3962719 .000
5.625191 8.136978
DM + S450 42.8280004
.3962719 .000
41.572107 44.083894
DM + S300 Normal 50.2859716
.3962719 .000
49.030078 51.541865
DM + S0 -8.6387734
.3962719 .000
-9.894667 -7.382880
DM + S150 -6.8810844
.3962719 .000
-8.136978 -5.625191
DM + S450 35.9469160
.3962719 .000
34.691022 37.202810
DM + S450 Normal 14.3390555
.3962719 .000
13.083162 15.594949
DM + S0 -44.5856894
.3962719 .000
-45.841583 -43.329796
DM + S150 -42.8280004
.3962719 .000
-44.083894 -41.572107
DM + S300 -35.9469160
.3962719 .000
-37.202810 -34.691022
. The mean difference is significant at the 0.01 level.
140 Lampiran 14 Profil Cu,Zn-SOD hati
Data pengamatan
Perlakuan Preparat
+++ ++
+- -
26 42
88 11
I 20
49 78
15 20
53 90
11 22
45 94
12 Normal
II 23
53 80
13 18
51 92
13 22
49 98
11 III
22 45
92 12
19 52
96 9
2 42
98 I
5 43
98 3
46 105
4 44
108 DM + S0
II 3
45 100
4 46
103 5
48 106
III 3
43 104
2 50
103 1
5 56
103 I
6 52
92 5
49 100
4 53
99 DM + S150
II 5
54 96
5 57
102 6
57 104
III 5
50 98
5 54
100 1
10 66
82 I
1 13
68 86
11 61
80 12
63 85
DM + S300 II
1 11
63 81
1 12
68 88
1 12
59 79
III 12
62 80
1 11
64 82
4 24
105 35
I 2
24 108
29 4
29 109
31 3
23 107
37 DM + S450
II 5
26 110
34 4
21 113
33 5
21 108
28 III
4 29
105 25
5 24
106 32
141
Analisis keragaman profil positif kuat +++ Cu,Zn-SOD hati
Descriptives
Profil Kuat N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 12.4403
.6168312 .35612
10.908043 13.972630
11.9611 13.1363 DM + S0
3 .0000
.0000000 .00000
.000000 .000000
.0000 .0000
DM + S150 3
.0673 .1166364
.06734 -.222401
.357081 .0000
.2020 DM + S300
3 .4168
.0129172 .00745
.384809 .448985
.4081 .4317
DM + S450 3
2.3795 .4387243
.25329 1.289652
3.469355 1.9734
2.8448 Total
15 3.0608
4.9457891 1.27699
.321929 5.799702
.0000 13.1363 ANOVA
Profil kuat Sum of Squares
df Mean Square
F Sig.
Between Groups 341.278
4 85.320
727.076 .000
Within Groups 1.173
10 .117
Total 342.452
14
Post Hoc Tests
Multiple Comparisons
Profil kuat LSD
I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Normal
DM + S0 12.4403362
.2796976 .000
11.553898 13.326774
DM + S150 12.3729962
.2796976 .000
11.486558 13.259434
DM + S300 12.0234392
.2796976 .000
11.137001 12.909877
DM + S450 10.0608324
.2796976 .000
9.174394 10.947270
DM + S0 Normal
-12.4403362 .2796976
.000 -13.326774
-11.553898 DM + S150
-.0673401 .2796976
.815 -.953778
.819098 DM + S300
-.4168971 .2796976
.167 -1.303335
.469541 DM + S450
-2.3795038 .2796976
.000 -3.265942
-1.493066 DM + S150 Normal
-12.3729962 .2796976
.000 -13.259434
-11.486558 DM + S0
.0673401 .2796976
.815 -.819098
.953778 DM + S300
-.3495570 .2796976
.240 -1.235995
.536881 DM + S450
-2.3121638 .2796976
.000 -3.198602
-1.425726 DM + S300 Normal
-12.0234392 .2796976
.000 -12.909877
-11.137001 DM + S0
.4168971 .2796976
.167 -.469541
1.303335 DM + S150
.3495570 .2796976
.240 -.536881
1.235995 DM + S450
-1.9626068 .2796976
.000 -2.849045
-1.076169 DM + S450 Normal
-10.0608324 .2796976
.000 -10.947270
-9.174394 DM + S0
2.3795038 .2796976
.000 1.493066
3.265942 DM + S150
2.3121638 .2796976
.000 1.425726
3.198602 DM + S300
1.9626068 .2796976
.000 1.076169
2.849045 . The mean difference is significant at the 0.01 level.
142 Analisis keragaman profil positif moderat ++ Cu,Zn-SOD hati
Descriptives
Profil moderat N
Mean Std.
Deviation Std. Error 95 Confidence Interval for Mean
Min Max
Lower Bound Upper Bound
Normal 3
28.40252 .62845
.36283 26.841350
29.963695 27.6945
28.8943 DM + S0
3 2.269072
.12864 .07427
1.949507 2.588636
2.1450 2.4018
DM + S150 3
3.213411 .24439
.14110 2.606292
3.820530 2.9462
3.4257 DM + S300
3 7.290819
.24782 .14307
6.675196 7.906442
7.0881 7.5671
DM + S450 3
14.618390 .92657
.53495 12.316665
16.920121 13.5558
15.2575 Total
15 11.158840 10.00742
2.58390 5.616916
16.700771 2.1450
28.8943
ANOVA
Profil moderat Sum of Squares
df Mean Square
F Sig.
Between Groups 1399.297
4 349.824 1.257E3
.000 Within Groups
2.782 10
.278 Total
1402.080 14
Post Hoc Tests
Multiple Comparisons
Profil moderat LSD
I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
99 Confidence Interval Lower Bound
Upper Bound Normal
DM + S0 26.1334508
.4306870 .000
24.768486 27.498415
DM + S150 25.1891115
.4306870 .000
23.824147 26.554076
DM + S300 21.1117034
.4306870 .000
19.746739 22.476668
DM + S450 13.7841298
.4306870 .000
12.419165 15.149094
DM + S0 Normal
-26.1334508 .4306870
.000 -27.498415
-24.768486 DM + S150
-.9443393 .4306870
.053 -2.309304
.420625 DM + S300
-5.0217474 .4306870
.000 -6.386712
-3.656783 DM + S450
-12.3493210 .4306870
.000 -13.714285
-10.984356 DM + S150 Normal
-25.1891115 .4306870
.000 -26.554076
-23.824147 DM + S0
.9443393 .4306870
.053 -.420625
2.309304 DM + S300
-4.0774082 .4306870
.000 -5.442373
-2.712444 DM + S450
-11.4049817 .4306870
.000 -12.769946
-10.040017 DM + S300 Normal
-21.1117034 .4306870
.000 -22.476668
-19.746739 DM + S0
5.0217474 .4306870
.000 3.656783
6.386712 DM + S150
4.0774082 .4306870
.000 2.712444
5.442373 DM + S450
-7.3275736 .4306870
.000 -8.692538
-5.962609 DM + S450 Normal
-13.7841298 .4306870
.000 -15.149094
-12.419165 DM + S0
12.3493210 .4306870
.000 10.984356
13.714285 DM + S150
11.4049817 .4306870
.000 10.040017
12.769946 DM + S300
7.3275736 .4306870
.000 5.962609
8.692538 . The mean difference is significant at the 0.01 level.
143
Analisis keragaman profil positif lemah +- Cu,Zn-SOD hati
Descriptives
Profil Lemah N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 5.221156E1
1.8075481 1.0435884E0
47.721365 56.701761
50.8556 54.2637
DM + S0 3
2.985433E1 .4491204
.2592998 28.738651
30.970005 29.5586
30.3711 DM + S150
3 3.364025E1
.8127906 .4692649
31.621167 35.659334
32.9229 34.5231
DM + S300 3
4.021694E1 .4241240
.2448681 39.163353
41.270518 39.9463
40.7057 DM + S450
3 6.424450E1
.5267256 .3041052
62.936045 65.552963
63.9211 64.8523
Total 15
4.403352E1 13.1099039
3.3849626E0 36.773494
51.293539 29.5586
64.8523
ANOVA
Profil lemah Sum of Squares
df Mean Square
F Sig.
Between Groups 2397.000
4 599.250
653.221 .000
Within Groups 9.174
10 .917
Total 2406.174
14
Post Hoc Tests
Multiple Comparisons
Profil lemah LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
22.3572349 .7820391
.000 19.878740
24.835730 DM + S150
18.5713123 .7820391
.000 16.092817
21.049808 DM + S300
11.9946272 .7820391
.000 9.516132
14.473122 DM + S450
-12.0329413 .7820391
.000 -14.511436
-9.554446 DM + S0
Normal -22.3572349
.7820391 .000
-24.835730 -19.878740
DM + S150 -3.7859225
.7820391 .001
-6.264418 -1.307427
DM + S300 -10.3626077
.7820391 .000
-12.841103 -7.884113
DM + S450 -34.3901761
.7820391 .000
-36.868671 -31.911681
DM + S150 Normal -18.5713123
.7820391 .000
-21.049808 -16.092817
DM + S0 3.7859225
.7820391 .001
1.307427 6.264418
DM + S300 -6.5766851
.7820391 .000
-9.055180 -4.098190
DM + S450 -30.6042536
.7820391 .000
-33.082749 -28.125758
DM + S300 Normal -11.9946272
.7820391 .000
-14.473122 -9.516132
DM + S0 10.3626077
.7820391 .000
7.884113 12.841103
DM + S150 6.5766851
.7820391 .000
4.098190 9.055180
DM + S450 -24.0275684
.7820391 .000
-26.506064 -21.549073
DM + S450 Normal 12.0329413
.7820391 .000
9.554446 14.511436
DM + S0 34.3901761
.7820391 .000
31.911681 36.868671
DM + S150 30.6042536
.7820391 .000
28.125758 33.082749
DM + S300 24.0275684
.7820391 .000
21.549073 26.506064
. The mean difference is significant at the 0.01 level.
144 Analisis keragaman profil negatif - Cu,Zn-SOD hati
Descriptives
Profil negatif N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 6.945578E0
.7490369 .4324566
5.084867 8.806289
6.0808 7.3893
DM + S0 3
6.787660E1 .3417526
.1973110 67.027640
68.725561 67.4839
68.1064 DM + S150
3 6.239734E1
.5785501 .3340260
60.960137 63.834533
61.7637 62.8975
DM + S300 3
5.207535E1 .2879956
.1662744 51.359927
52.790769 51.7981
52.3730 DM + S450
3 1.875760E1
1.4535192 .8391897
15.146857 22.368341
17.2610 20.1638
Total 15
4.161049E1 25.1746653
6.5000706E0 27.669227
55.551757 6.0808
68.1064
ANOVA
Profil negatif Sum of Squares
df Mean Square
F Sig.
Between Groups 8866.276
4 2216.569 3.455E3
.000 Within Groups
6.416 10
.642 Total
8872.693 14
Post Hoc Tests
Multiple Comparisons
Profil negatif LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
-60.9310219 .6540368
.000 -63.003843
-58.858201 DM + S150
-55.4517568 .6540368
.000 -57.524578
-53.378936 DM + S300
-45.1297697 .6540368
.000 -47.202591
-43.056949 DM + S450
-11.8120210 .6540368
.000 -13.884842
-9.739200 DM + S0
Normal 60.9310219
.6540368 .000
58.858201 63.003843
DM + S150 5.4792651
.6540368 .000
3.406444 7.552086
DM + S300 15.8012522
.6540368 .000
13.728431 17.874073
DM + S450 49.1190009
.6540368 .000
47.046180 51.191822
DM + S150 Normal 55.4517568
.6540368 .000
53.378936 57.524578
DM + S0 -5.4792651
.6540368 .000
-7.552086 -3.406444
DM + S300 10.3219870
.6540368 .000
8.249166 12.394808
DM + S450 43.6397358
.6540368 .000
41.566915 45.712557
DM + S300 Normal 45.1297697
.6540368 .000
43.056949 47.202591
DM + S0 -15.8012522
.6540368 .000
-17.874073 -13.728431
DM + S150 -10.3219870
.6540368 .000
-12.394808 -8.249166
DM + S450 33.3177488
.6540368 .000
31.244928 35.390570
DM + S450 Normal 11.8120210
.6540368 .000
9.739200 13.884842
DM + S0 -49.1190009
.6540368 .000
-51.191822 -47.046180
DM + S150 -43.6397358
.6540368 .000
-45.712557 -41.566915
DM + S300 -33.3177488
.6540368 .000
-35.390570 -31.244928
. The mean difference is significant at the 0.01 level.
145 Lampiran 15 Aktivitas katalase
Data pengamatan
Perlakuan Menit ke-
Beda serapan
Rerata 1
2 3
Normal-1 0,014
0,015 0,015
0,0147 0,0152
0,015 0,016
0,016 0,0157
Normal-2 0,012
0,012 0,013
0,0123 0,0127
0,013 0,013
0,013 0,0130
Normal-3 0,016
0,016 0,016
0,0160 0,0160
0,016 0,016
0,016 0,0160
Normal-4 0,016
0,016 0,016
0,0160 0,0165
0,017 0,017
0,017 0,0170
Normal-5 0,015
0,015 0,015
0,0150 0,0150
0,015 0,015
0,015 0,0150
Normal-6 0,015
0,015 0,016
0,0153 0,0142
0,013 0,013
0,013 0,0130
DM + S0 -1 0,269
0,269 0,269
0,2690 0,2678
0,266 0,267
0,267 0,2667
DM + S0 -2 0,271
0,271 0,271
0,2710 0,2697
0,268 0,268
0,269 0,2683
DM + S0 -3 0,265
0,265 0,265
0,2650 0,2653
0,265 0,266
0,266 0,2657
DM + S0 -4 0,263
0,263 0,263
0,2630 0,2633
0,263 0,264
0,264 0,2637
DM + S150 -1 0,225
0,225 0,226
0,2253 0,2262
0,227 0,227
0,227 0,2270
DM + S150 -2 0,219
0,219 0,219
0,2190 0,2195
0,22 0,22
0,22 0,2200
DM + S150 -3 0,219
0,22 0,22
0,2197 0,2205
0,221 0,221
0,222 0,2213
DM + S150 -4 0,223
0,223 0,223
0,2230 0,2240
0,225 0,225
0,225 0,2250
DM + S300 -1 0,165
0,165 0,166
0,1653 0,1667
0,168 0,168
0,168 0,1680
DM + S300 -2 0,172
0,172 0,172
0,1720 0,1713
0,170 0,171
0,171 0,1707
DM + S300 -3 0,163
0,163 0,163
0,1630 0,1625
0,162 0,162
0,162 0,1620
DM + S300 -4 0,164
0,164 0,164
0,1640 0,1650
0,166 0,166
0,166 0,1660
DM + S450 -1 0,094
0,094 0,095
0,0943 0,0932
0,092 0,092
0,092 0,0920
DM + S450 -2 0,096
0,096 0,096
0,0960 0,0970
0,098 0,098
0,098 0,0980
DM + S450 -3 0,100
0,100 0,101
0,1003 0,1012
0,102 0,102
0,102 0,1020
DM + S450 -4 0,098
0,098 0,099
0,0983 0,0987
0,099 0,099
0,099 0,0990
DM + S450 -5 0,097
0,097 0,097
0,0970 0,0965
0,096 0,096
0,096 0,0960
DM + S450 -6 0,103
0,0103 0,103
0,0721 0,0854
0,098 0,099
0,099 0,0987
Konsentrasi Standar 0,04
0,08 0,12
0,16 0,2
0,4
Serapan 0,025
0,146 0,223
0,302 0,375
0,464 0,994
0,031 0,134
0,234 0,298
0,368 0,472
1,021
146
y = 2.4021x + 0.0202 R
2
= 0.9938
0.2 0.4
0.6 0.8
1 1.2
0.05 0.1
0.15 0.2
0.25 0.3
0.35 0.4
0.45
Hasil perhitungan
Perlakuan Molar molL
μmolminmL
1 -0,00300
0,18525457 185,2546
2 -0,00397
0,18622594 186,2259
Normal 3
-0,00258 0,18483827
184,8383 4
-0,00237 0,18463012
184,6301 5
-0,00307 0,18532395
185,3240 6
-0,00334 0,18560149
185,6015 1
0,10309 0,07916684
79,1668 DM + S0
2 0,10385
0,07840362 78,4036
3 0,10205
0,08020760 80,2076
4 0,10122
0,08104020 81,0402
1 0,08574
0,09651277 96,5128
DM + S150 2
0,08297 0,09928812
99,2881 3
0,08339 0,09887182
98,8718 4
0,08484 0,09741476
97,4148 1
0,06097 0,12128277
121,2828 DM + S300
2 0,06292
0,11934002 119,3400
3 0,05924
0,12301736 123,0174
4 0,06028
0,12197660 121,9766
1 0,03038
0,15188099 151,8810
2 0,03197
0,15028517 150,2852
DM + S450 3
0,03371 0,14855057
148,5506 4
0,03267 0,14959133
149,5913 5
0,03176 0,15049332
150,4933 6
0,02714 0,15512121
155,1212
Data aktivitas katalase tikus percobaan
Perlakuan 1
2 3
4 5
6
Normal 185,2546
186,2259 184,8383
184,6301 185,3240
185,6015 DM + S0
66,6777 65,7185
67,7185 68,5511
- -
DM + S150 96,5127
99,2881 98,8718
97,4147 -
- DM + S300
121,2828 119,3400
123,0174 121,9766
- -
DM + S450 164,3701
162,7742 161,0396
162,0804 162,9824
161,1784
147 Analisis keragaman aktivitas katalase tikus percobaan
Descriptives
Katalase N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
6 1.853124E2
.5677747 .2317931
184.716557 185.908243 184.6301 186.2259
DM + S0 4
6.716648E1 1.2325352
.6162676 65.205246
69.127724 65.7185
68.5511 DM + S150
4 9.802187E1
1.2873447 .6436724
95.973415 100.070320
96.5128 99.2881
DM + S300 4
1.214042E2 1.5498081
.7749041 118.938109
123.870291 119.3400 123.0174 DM + S450
6 1.624042E2
1.2494433 .5100831
161.092973 163.715394 161.0396 164.3701
Total 24
1.346946E2 43.9032186
8.9617070E0 116.155868
153.233275 65.7185 186.2259
ANOVA
Katalase Sum of Squares
df Mean Square
F Sig.
Between Groups 44306.178
4 11076.544 8.047E3
.000 Within Groups
26.152 19
1.376 Total
44332.330 23
Post Hoc Tests
Multiple Comparisons
Katalase LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
118.1459150 .7573079
.000 115.979307
120.312523 DM + S150
87.2905325 .7573079
.000 85.123924
89.457141 DM + S300
63.9082000 .7573079
.000 61.741592
66.074808 DM + S450
22.9082167 .6773568
.000 20.970343
24.846090 DM + S0
Normal -1.1814592E2
.7573079 .000
-120.312523 -115.979307
DM + S150 -30.8553825
.8295893 .000
-33.228783 -28.481982
DM + S300 -54.2377150
.8295893 .000
-56.611116 -51.864314
DM + S450 -95.2376983
.7573079 .000
-97.404307 -93.071090
DM + S150 Normal -87.2905325
.7573079 .000
-89.457141 -85.123924
DM + S0 30.8553825
.8295893 .000
28.481982 33.228783
DM + S300 -23.3823325
.8295893 .000
-25.755733 -21.008932
DM + S450 -64.3823158
.7573079 .000
-66.548924 -62.215707
DM + S300 Normal -63.9082000
.7573079 .000
-66.074808 -61.741592
DM+S0 54.2377150
.8295893 .000
51.864314 56.611116
DM+S150 23.3823325
.8295893 .000
21.008932 25.755733
DM+S450 -40.9999833
.7573079 .000
-43.166592 -38.833375
DM + S450 Normal -22.9082167
.6773568 .000
-24.846090 -20.970343
DM + S0 95.2376983
.7573079 .000
93.071090 97.404307
DM + S150 64.3823158
.7573079 .000
62.215707 66.548924
DM + S300 40.9999833
.7573079 .000
38.833375 43.166592
. The mean difference is significant at the 0.01 level.
148 Lampiran 16 Aktivitas glutation peroksidase GSH-Px
Data pengamatan
Perlakuan Menit ke-
Beda serapan
Rerata 1
2 3
1 0,037
0,038 0,039
0,0010 0,00075
0,035 0,035
0,036 0,0005
2 0,037
0,037 0,038
0,0005 0,00075
0,034 0,035
0,036 0,0010
Normal 3
0,036 0,037
0,038 0,0010
0,00075 0,035
0,035 0,036
0,0005 4
0,038 0,039
0,039 0,0005
0,00075 0,035
0,036 0,037
0,0010 5
0,036 0,036
0,037 0,0005
0,00075 0,034
0,035 0,036
0,0010 6
0,037 0,038
0,039 0,0010
0,00100 0,036
0,037 0,038
0,0010 1
0,010 0,010
0,011 0,0005
0,00025 0,011
0,011 0,011
0,0000 2
0,011 0,012
0,012 0,0005
0,00025 DM + S0
0,011 0,011
0,011 0,0000
3 0,010
0,011 0,011
0,0005 0,00025
0,010 0,010
0,010 0,0000
4 0,012
0,012 0,012
0,0000 0,00025
0,010 0,010
0,011 0,0005
1 0,017
0,016 0,017
0,0000 0,00025
0,016 0,017
0,017 0,0005
2 0,016
0,017 0,017
0,0005 0,00050
DM + S150 0,017
0,017 0,018
0,0005 3
0,017 0,018
0,018 0,0005
0,00050 0,017
0,017 0,018
0,0005 4
0,015 0,015
0,016 0,0005
0,00025 0,015
0,015 0,015
0,0000 1
0,020 0,020
0,021 0,0005
0,00050 0,020
0,020 0,021
0,0005 2
0,021 0,022
0,022 0,0005
0,00025 DM + S300
0,023 0,023
0,023 0,0000
3 0,022
0,022 0,023
0,0005 0,00050
0,020 0,021
0,021 0,0005
4 0,023
0,023 0,024
0,0005 0,00075
0,020 0,021
0,022 0,0010
1 0,029
0,029 0,030
0,0005 0,00050
0,028 0,028
0,029 0,0005
2 0,030
0,030 0,031
0,0005 0,00050
0,028 0,028
0,029 0,0005
3 0,028
0,028 0,029
0,0005 0,00050
DM + S450 0,029
0,029 0,030
0,0005 4
0,028 0,029
0,030 0,0010
0,00075 0,027
0,027 0,028
0,0005 5
0,029 0,029
0,030 0,0005
0,00050 0,028
0,028 0,029
0,0005 6
0,028 0,028
0,030 0,0010
0,00100 0,029
0,030 0,031
0,0010
Data aktivitas GSH-Px
Perlakuan 1
2 3
4 5
6
Normal 2,4116
2,4116 2,4116
2,4116 2,4116
3,2154 DM + S0
0,8039 0,8039
0,8039 0,8039
- -
DM + S150 0,8039
1,6077 1,6077
0,8039 -
- DM + S300
1,6077 0,8039
1,7077 2,4116
- -
DM + S450 1,6077
1,6077 1,6077
2,4116 1,6077
3,2154
149 Analisis keragaman aktivitas GSH-Px tikus percobaan
Descriptives
GSH-Px N
Mean Std.
Deviation Std. Error 95 Confidence Interval for Mean
Min Max
Lower Bound Upper Bound
Normal 6
2.545567E0 .3281500 .1339667 2.201194
2.889939 2.4116
3.2154 DM+S0
4 .803900 .0000000 .0000000
.803900 .803900
.8039 .8039
DM+S150 4
1.205800E0 .4640741 .2320371 .467354
1.944246 .8039
1.6077 DM+S300
4 1.632725E0 .6582413 .3291206
.585316 2.680134
.8039 2.4116
DM+S450 6
2.009633E0 .6725551 .2745695 1.303830
2.715437 1.6077
3.2154 Total
24 1.745871E0 .7736885 .1579285
1.419171 2.072571
.8039 3.2154
ANOVA
GSH-Px Sum of Squares
df Mean Square
F Sig.
Between Groups 9.022
4 2.255
9.029 .000
Within Groups 4.746
19 .250
Total 13.768
23
Post Hoc Tests
Multiple Comparisons
GSH-Px LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM+S0
1.7416667 .3226128
.000 .818693
2.664641 DM+S150
1.3397667 .3226128
.001 .416793
2.262741 DM+S300
.9128417 .3226128
.011 -.010132
1.835816 DM+S450
.5359333 .2885537
.079 -.289600
1.361466 DM+S0
Normal -1.7416667
.3226128 .000
-2.664641 -.818693
DM+S150 -.4019000
.3534046 .270
-1.412967 .609167
DM+S300 -.8288250
.3534046 .030
-1.839892 .182242
DM+S450 -1.2057333
.3226128 .001
-2.128707 -.282759
DM+S150 Normal
-1.3397667 .3226128
.001 -2.262741
-.416793 DM+S0
.4019000 .3534046
.270 -.609167
1.412967 DM+S300
-.4269250 .3534046
.242 -1.437992
.584142 DM+S450
-.8038333 .3226128
.022 -1.726807
.119141 DM+S300
Normal -.9128417
.3226128 .011
-1.835816 .010132
DM+S0 .8288250
.3534046 .030
-.182242 1.839892
DM+S150 .4269250
.3534046 .242
-.584142 1.437992
DM+S450 -.3769083
.3226128 .257
-1.299882 .546066
DM+S450 Normal
-.5359333 .2885537
.079 -1.361466
.289600 DM+S0
1.2057333 .3226128
.001 .282759
2.128707 DM+S150
.8038333 .3226128
.022 -.119141
1.726807 DM+S300
.3769083 .3226128
.257 -.546066
1.299882 . The mean difference is significant at the 0.01 level.
150 Lampiran 17 Vasorelaksasi
Data pengamatan vasorelaksasi
Perlakuan Base line
Max Min
Kontraksi Relaksasi
1 0,005
0,040 0,008
0,035 0,032
2 -0,005
0,107 -0,002
0,112 0,109
Normal 3
0,010 0,075
0,017 0,065
0,058 4
-0,002 0,082
0,004 0,084
0,078 5
-0,004 0,059
0,002 0,063
0,057 6
0,003 0,098
0,012 0,095
0,086 1
-0,001 0,043
0,036 0,044
0,007 DM + S0
2 0,006
0,023 0,002
0,017 0,003
3 -0,006
0,013 0,009
0,019 0,004
4 0,005
0,071 0,069
0,066 0,002
1 0,007
0,128 0,100
0,121 0,028
DM + S150 2
0,004 0,063
0,049 0,059
0,014 3
-0,001 0,076
0,058 0,077
0,018 4
-0,005 0,077
0,057 0,082
0,020 1
0,007 0,007
0,046 0,063
0,024 DM + S300
2 -0,006
0,086 0,049
0,092 0,037
3 -0,026
0,006 0,025
0,086 0,035
4 -0,004
0,002 0,010
0,024 0,010
1 0,006
0,056 0,033
0,050 0,023
2 0,000
0,082 0,041
0,082 0,041
DM + S450 3
-0,002 0,119
0,034 0,121
0,085 4
0,003 0,034
0,011 0,031
0,023 5
-0,004 0,063
0,010 0,067
0,053 6
0,004 0,044
0,009 0,040
0,035
Data vasorelaksasi
Perlakuan 1
2 3
4 5
6
Normal 91,4286
97,3214 89,2308
92,8571 90,4762
90,5263 DM + S0
15,9091 17,6471
21,0526 3,0303
- -
DM + S150 23,1405
23,7288 23,3766
24,3902 -
- DM + 300
38,0952 40,2174
40,6977 41,6667
- -
DM + 450 46,0000
50,0000 70,2479
74,1936 79,1045
87,5000
Analisis keragaman vasorelaksasi tikus percobaan
Descriptives
Vasorelaksasi
N Mean
Std. Deviation
Std. Error 95 Confidence Interval for Mean
Minimum Maximum
Lower Bound Upper Bound
Normal 6
91.9734 2.8822
1.1766 88.94866261
94.99814406 8.923077E1 9.732143E1 DM+S0
4 14.4098
7.8813 3.9406
1.86875617 26.95078533
3.030303 2.105263E1 DM+S150
6 67.8409
16.4609 6.7201
50.56624332 85.11574335 4.600000E1 8.750000E1
DM+S300 4
40.1692 1.5083
.7542 37.76912631
42.56935869 3.809524E1 4.166667E1 DM+S450
4 23.6590
.5441 .2721
22.79324256 24.52484244 2.314050E1 2.439024E1
Total 24
52.9933 30.9869
6.3251 39.90862930
66.07792095 3.030303 9.732143E1
151
ANOVA
Vasorelaksasi Sum of Squares
df Mean Square
F Sig.
Between Groups 20493.990
4 5123.498
61.208 .000
Within Groups 1590.419
19 83.706
Total 22084.409
23
Post Hoc Tests
Multiple Comparisons
Vasorelaksasi LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
77.5636326 5.9057275E0
.000 60.667732
94.459533 DM + S150
68.3143608 5.9057275E0
.000 51.418461
85.210261 DM + S300
51.8041608 5.9057275E0
.000 34.908261
68.700061 DM + S450
24.1324100 5.2822433E0
.000 9.020257
39.244563 DM + S0
Normal -77.5636326
5.9057275E0 .000
-94.459533 -60.667732
DM + S150 -9.2492717
6.4694003E0 .169
-27.757803 9.259260
DM + S300 -25.7594718
6.4694003E0 .001
-44.268003 -7.250940
DM + S450 -53.4312226
5.9057275E0 .000
-70.327123 -36.535322
DM + S150 Normal -68.3143608
5.9057275E0 .000
-85.210261 -51.418461
DM + S0 9.2492717
6.4694003E0 .169
-9.259260 27.757803
DM + S300 -16.5102000
6.4694003E0 .019
-35.018731 1.998331
DM + S450 -44.1819508
5.9057275E0 .000
-61.077851 -27.286051
DM + S300 Normal -51.8041608
5.9057275E0 .000
-68.700061 -34.908261
DM + S0 25.7594718
6.4694003E0 .001
7.250940 44.268003
DM + S150 16.5102000
6.4694003E0 .019
-1.998331 35.018731
DM + S450 -27.6717508
5.9057275E0 .000
-44.567651 -10.775851
DM + S450 Normal -24.1324100
5.2822433E0 .000
-39.244563 -9.020257
DM + S0 53.4312226
5.9057275E0 .000
36.535322 70.327123
DM + S150 44.1819508
5.9057275E0 .000
27.286051 61.077851
DM + S300 27.6717508
5.9057275E0 .000
10.775851 44.567651
. The mean difference is significant at the 0.01 level.
152 Lampiran 18 Dosis Efektif ED
50
Perlakuan
Data pengamatan dosis efektif
Dosis asetilkolin log M -7
-6,5 -6
-5,5 -5
-4,5 -4
-3,5
Normal - 1 34,3750
46,8750 53,1250
65,6250 78,1250
90,6250 96,8750
100,0000 Normal - 2
0,9174 53,2110
100,0000 100,0000
100,0000 100,0000
100,0000 100,0000
Normal - 3 3,4483
5,1724 98,2759
100,0000 100,0000
100,0000 100,0000
100,0000 Normal - 4
15,3846 34,6154
96,1539 100,0000
100,0000 100,0000
100,0000 100,0000
Normal - 5 14,0351
42,1053 71,9298
82,4561 100,0000
100,0000 100,0000
100,0000 Normal - 6
15,1163 45,3488
63,9535 86,0465
91,8605 100,0000
100,0000 100,0000
DM + S0 - 1 16,6667
25,0000 33,3333
41,6667 66,6667
66,6667 100,0000
100,0000 DM + S0 – 2
0,0000 0,0000
0,0000 33,3333
100,0000 100,0000
100,0000 100,0000
DM + S0 – 3 0,0000
25,0000 25,0000
100,0000 100,0000
100,0000 100,0000
100,0000 DM + S0 – 4
0,0000 0,0000
0,0000 50,0000
100,0000 100,0000
100,0000 100,0000
DM + S150 – 1 3,5714
21,4286 57,1429
67,8571 78,5714
85,7143 92,8571
100,0000 DM + S150 – 2
3,5714 14,2857
35,7143 42,8571
100,0000 100,0000
100,0000 100,0000
DM + S150 – 3 5,5556
11,1111 33,3333
61,1111 77,7778
100,0000 100,0000
100,0000 DM + S150 - 4
5,0000 15,0000
35,0000 70,0000
85,0000 100,0000
100,0000 100,0000
DM + S300 – 1 0,0000
4,1667 50,0000
75,0000 87,50000
100,0000 100,0000
100,0000 DM + S300 – 2
2,7027 16,2162
51,3514 91,8919
100,0000 100,0000
100,0000 100,0000
DM + S300 - 3 5,7143
25,7143 34,2857
80,0000 100,0000
100,0000 100,0000
100,0000 DM + S300 - 4
10,0000 20,0000
30,0000 100,0000
100,0000 100,0000
100,0000 100,0000
DM + S450 - 1 8,0000
20,0000 52,0000
100,0000 100,0000
100,0000 100,0000
100,0000 DM + S450 – 2
7,3171 39,0244
58,5366 78,0488
87,8049 95,1219
100,0000 100,0000
DM + S450 – 3 15,2941
38,8235 47,0588
60,0000 80,0000
92,9412 100,0000
100,0000 DM + S450 - 4
8,6957 21,7391
52,1739 100,0000
100,0000 100,0000
100,0000 100,0000
DM + S450 – 5 18,8679
58,4906 100,0000
100,0000 100,0000
100,0000 100,0000
100,0000 DM + S450 - 6
5,7143 11,4286
42,8571 100,0000
100,0000 100,0000
100,0000 100,0000
Data ED
Perlakuan
50
1 2
3 4
5 6
Normal -5,395
-7,258 -6,523
-6,668 -6,416
-6,333 DM + S0
-4,973 -5,434
-6,004 -5,541
- -
DM + S150 -5,849
-5,580 -5,641
-5,786 -
- DM + S300
-6,026 -6,158
-5,897 -5,970
- -
DM + S450 -6,178
-6,257 -5,566
-6,180 -7,137
-6,089
153 Analisis keragaman ED
50
tikus percobaan
Descriptives
ED
50
N Mean
Std. Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
6 -6.432167E0 .6052568 .2470950
-7.067345 -5.796989
-7.2580 -5.3950
DM + S0 4
-5.488000E0 .4231651 .2115825 -6.161350
-4.814650 -6.0040
-4.9730 DM + S150
4 -5.714000E0 .1247584 .0623792
-5.912519 -5.515481
-5.8490 -5.5800
DM + S300 4
-6.012750E0 .1103007 .0551504 -6.188263
-5.837237 -6.1580
-5.8970 DM + S450
6 -6.234500E0 .5077869 .2073031
-6.767390 -5.701610
-7.1370 -5.5660
Total 24
-6.035792E0 .5331420 .1088272 -6.260918
-5.810666 -7.2580
-4.9730
ANOVA ED
50
Sum of Squares df
Mean Square F
Sig. Between Groups
2.796 4
.699 3.550
.025 Within Groups
3.741 19
.197 Total
6.538 23
Post Hoc Tests
Multiple Comparisons
ED
50
LSD I Perlakuan
J Perlakuan
Mean Difference I-J
Std. Error Sig.
95 Confidence Interval Lower Bound
Upper Bound Normal
DM + S0 -.9441667
.2864374 .004
-1.543687 -.344646
DM + S150 -.7181667
.2864374 .021
-1.317687 -.118646
DM + S300 -.4194167
.2864374 .159
-1.018937 .180104
DM + S450 -.1976667
.2561974 .450
-.733894 .338561
DM + S0 Normal
.9441667 .2864374
.004 .344646
1.543687 DM + S150
.2260000 .3137764
.480 -.430742
.882742 DM + S300
.5247500 .3137764
.111 -.131992
1.181492 DM + S450
.7465000 .2864374
.017 .146980
1.346020 DM + S150 Normal
.7181667 .2864374
.021 .118646
1.317687 DM + S0
-.2260000 .3137764
.480 -.882742
.430742 DM + S300
.2987500 .3137764
.353 -.357992
.955492 DM + S450
.5205000 .2864374
.085 -.079020
1.120020 DM+S300
Normal .4194167
.2864374 .159
-.180104 1.018937
DM+S0 -.5247500
.3137764 .111
-1.181492 .131992
DM+S150 -.2987500
.3137764 .353
-.955492 .357992
DM+S450 .2217500
.2864374 .448
-.377770 .821270
DM + S450 Normal .1976667
.2561974 .450
-.338561 .733894
DM + S0 -.7465000
.2864374 .017
-1.346020 -.146980
DM + S150 -.5205000
.2864374 .085
-1.120020 .079020
DM + S300 -.2217500
.2864374 .448
-.821270 .377770
. The mean difference is significant at the 0.05 level.
154 Lampiran 19 Rasio Sel Endot elium
Data pengamatan
Perlakuan Keliling
aorta µm
Jumlah panjang sel endotelium
µm Rasio sel
endotelium
Normal - 1 25846,27
25499,1573 98,65701
Normal - 2 26754,33
25768,5900 96,31559
Normal - 3 24582,90
24153,9000 98,25488
DM + S0-1 26151,98
18135,1894 69,34538
DM + S0-2 28422,12
21944,3376 77,20866
DM + S0-3 23782,03
11776,1282 49,51692
DM + S150-1 23795,01
15852,6430 66,62171
DM + S150-2 30914,14
21684,7270 70,14501
DM + S150-3 26953,58
18966,1360 70,36593
DM + S300-1 26644,21
21291,4973 79,91041
DM + S300-2 23832,42
19491,5978 81,78606
DM + S300-3 27526,08
22775,0985 82,74007
DM + S450-1 26721,99
24136,8289 90,32572
DM + S450-2 27312,43
24539,7020 89,84811
DM + S450-3 24984,47
22332,1870 89,38427
Analisis keragaman rasio sel endotelium tikus percobaan
Descriptives
Rasio N
Mean Std.
Deviation Std. Error
95 Confidence Interval for Mean Min
Max Lower Bound
Upper Bound Normal
3 9.7742493E1 1.25198521
.72283400 94.6323896
100.8525970 96.31559 98.65701 DM + S0
3 6.5356987E1 14.27019892 8.23890319E0 29.9078474
100.8061259 49.51692 77.20866 DM + S150
3 6.9044217E1 2.10085824 1.21293107E0
63.8253955 74.2630378 66.62171 70.36593
DM + S300 3 8.1478847E1
1.43962806 .83116965
77.9026123 85.0550810 79.91041 82.74007
DM + S450 3 8.9852700E1
.47074178 .27178290
88.6833126 91.0220874 89.38427 90.32572
Total 15 8.0695049E1 13.78948489 3.56042969E0
73.0586865 88.3314109 49.51692 98.65701
ANOVA
Rasio Sum of Squares
df Mean Square
F Sig.
Between Groups 2238.271
4 559.568
13.203 .001
Within Groups 423.828
10 42.383
Total 2662.099
14
155 Post Hoc Tests
Multiple Comparisons
Rasio LSD
I Perlakuan J
Perlakuan Mean Difference
I-J Std. Error
Sig. 99 Confidence Interval
Lower Bound Upper Bound
Normal DM + S0
32.38550667 5.31555925E0
.000 15.5390500
49.2319633 DM + S150
28.69827667 5.31555925E0
.000 11.8518200
45.5447333 DM + S300
16.26364667 5.31555925E0 .012
-.5828100 33.1101033
DM + S450 7.88979333 5.31555925E0
.169 -8.9566633
24.7362500 DM + S0
Normal -3.23855067E1
5.31555925E0 .000
-49.2319633 -15.5390500
DM + S150 -3.68723000 5.31555925E0
.504 -20.5336867
13.1592267 DM + S300
-16.12186000 5.31555925E0 .013
-32.9683167 .7245967
DM + S450 -2.44957133E1
5.31555925E0 .001
-41.3421700 -7.6492567
DM + S150 Normal -2.86982767E1
5.31555925E0 .000
-45.5447333 -11.8518200
DM + S0 3.68723000 5.31555925E0
.504 -13.1592267
20.5336867 DM + S300
-12.43463000 5.31555925E0 .041
-29.2810867 4.4118267
DM + S450 -2.08084833E1
5.31555925E0 .003
-37.6549400 -3.9620267
DM + S300 Normal -16.26364667 5.31555925E0
.012 -33.1101033
.5828100 DM + S0
16.12186000 5.31555925E0 .013
-.7245967 32.9683167
DM + S150 12.43463000 5.31555925E0
.041 -4.4118267
29.2810867 DM + S450
-8.37385333 5.31555925E0 .146
-25.2203100 8.4726033
DM + S450 Normal -7.88979333 5.31555925E0
.169 -24.7362500
8.9566633 DM + S0
24.49571333 5.31555925E0
.001 7.6492567
41.3421700 DM + S150
20.80848333 5.31555925E0
.003 3.9620267
37.6549400 DM + S300
8.37385333 5.31555925E0 .146
-8.4726033 25.2203100
. The mean difference is significant at the 0.01 level.
ABSTRACT
MUHAMAD FIRDAUS. Antioxidant activity of brown algae Sargassum echinocarpum
as preventive on dysfunction of endothelium cell in aorta of diabetes mellitus rats. Under supervision of MADE ASTAWAN as chairman,
DEDDY MUCHTADI, TUTIK WRESDIYATI, SARWONO WASPADJI, and SETYAWATI S. KARYONO, as member committee.
Diabetes mellitus may cause oxidative stress. Chronic oxidative stress initiates
the dysfunction of endothelium cell. Polyphenols are believed to have ability to
prevent the dysfunction of endothelium cell. Marine brown algae contain polyphenol which is probably has good antioxidant activity.
The aims of this study were to obtain the antioxidant activity and structure of phlorotannin, the
lethal dose, the anti oxidative stress, and the anti endothelium cell dysfunction of Sargassum
echinocarpum extract. The Sargassum echinocarpum was collected in April 2008 from the coastline of Talango island in Sumenep District of East Java,
Indonesia and authenticated by the botanist, Research Centre of Oceanography, Indonesian Institute of Sciences. The antioxidant activity was assayed with DPPH
radicals, the structure elucidation was assessed with spectroscopic methods, the lethal dose was investigated on mice, and the anti oxidative stress and the anti
endothelium cell dysfunction were evaluated in diabetic rats. The experimental animals were divided into 5 groups, i.e.: 1 normal, 2 diabetic treated with 0, 3
150, 4 300, and 5 450 mgkg body weight of Sargassum echinocarpum extract, respectively. Extracts were administered by oral gavages for 12 weeks. Diabetes
was induced by single administration of streptozotocin 45 mgkg, i.p.. Diabetes was confirmed ten days later in streptozotocin induced animals with blood glucose
levels 200 mgdL. After the treatment period, the blood serum acquired was used for antioxidant enzymes assays while the thoracic aorta was used for
vasodilatation assay. The result showed that the phlorotannin content of Sargassum echinocarpum
extract was 4.25 - 12.16 equivalent of mg phloroglucinolg extract. The strongest antioxidant activity obtained from the
methanol extract of Sargassum echinocarpum, i.e. 67.77 . The methanol extract of Sargassum echinocarpum contained phloroglucinol and bifuhalol. The
methanol extract of Sargassum echinocarpum was relatively non-toxic. The mice treated with 1250 mgkg or more methanol extract of Sargassum echinocarpum
showed inhibition of body weight and necrosis on hepatocyte and tubules of kidney. It was affected by inhibition activity of gastrointestinal enzymes and
initiation lipid peroxidation. The diabetic rats revealed the oxidative stress; meanwhile, the diabetic rats treated with 450 mgkg
extract showed the mild oxidative stress. It was caused by free radical scavenging and induction of
antioxidant defense activity by extract. The diabetic rats exhibited the endothelial dysfunction; meanwhile, the diabetic rats treated with 450 mgkg
extract showed the mild endothelial dysfunction. It appeared that Sargassum echinocarpum
extract has the capability to preserve bioavailability of nitric oxide and protect the muscarinic receptor and endothelium cell against free radical damage.
Keywords: Antioxidant, diabetes mellitus, endothelium cell dysfunction, rat, Sargassum echinocarpum
1 PENDAHULUAN
1.1 Latar Belakang