74 dan P450 tidak tinggi. Pada perlakuan in-vivo proses deteksi dan detoksifikasi
terjadi secara alami di dalam tubuh serangga sehingga aktivitas sitokrom b5 dan sitokrom P450 menunjukkan aktivitas yang setara. Pada perlakuan in-vitro,
dimana senyawa asing ditemukan dalam jumlah besar maka aktivitas kedua enzim secara keseluruhan lebih tinggi dan sitokrom b5 juga lebih tinggi dibandingkan
sitokrom P450 Gambar 4.4.
4.4 Kesimpulan
Dilapiol merupakan senyawa utama pada ekstrak kasar dan ekstrak hasil distilasi P. aduncum dengan hasil berturut-turut sebanyak 75.18 dan 79.36.
Ekstrak campuran T. vogelii : P. aduncum 1:5 mengganggu fisiologi serangga melalui efek antifeedant sekunder dan gangguan asimilasi makanan C. pavonana.
Perlakuan ekstrak campuran T. vogelii : P. aduncum 1:5 pada LC
25
dan LC
50
menyebabkan gangguan pertumbuhan relatif akibat toksisitas intrinsik ekstrak campuran yang masuk ke dalam tubuh serangga. Keberadaan senyawa asing
meningkatkan daya cerna serangga sebagai bentuk efisiensi absorpsi nutrisi makanan. Senyawa aktif pada P. aduncum menyebabkan aktivitas enzim oksidasi
sitokrom b5 dan sitokrom P450 meningkat pada perlakuan in-vivo dan in-vitro jika dibandingkan dengan kontrol.
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