Preparation of standard curve

RESULT AND ANALYSIS Pandan wangi used in this study was obtained from Purwodadi. This study begins with the identification of pandan wangi will be used. This step aims to ensure that the plants used are not wrong and actually comes from Pandanus amaryllifolius Roxb species. The extraction method used in this research was maceration with ethanol 70 as solvent. Ethanol are neutral, can inhibit the growth of mold and bacteria, non-toxic, can be mixed with water at any ratio, and the time required for a concentration of less Depkes, 1986. So it is expected these solvents can obtain optimal active substances because it can attract active substances that dissolve in water or in alcohol. The amount of 70 ethanol used in this maceration is 10 times the weight of crude drugs. Pandan wangi leaves extract made with a series of levels of 0.625, 1.25, 2.5, 5 and 10, the selection is based on a series of levels of study Rahardjo with levels of 10 proved to have an influence on the solubility of calcium kidney stones. For further research then made a different dosage forms that extracts fragrant pandan In this case, the researchers only identify the elements which generally form kidney stones among other things carbonate, calcium, oxalate, and phosphate. However, the most important element in this study is calcium. For qualitative analysis of calcium, carried out by first adding calcium powder with 10 HCl. The addition of 10 HCl aims to dissolve the calcium. Selection of HCl 10 based on the theory that most of the compounds in the form of calcium kidney stones of calcium oxalate. This is evident in the analysis of oxalate which showed positive results. Calcium oxalate is only soluble in strong mineral acid HCl 10 so selected that a strong mineral acid as the solvent of calcium oxalate. After the addition of 10 HCL and heated, then the solution was added ammonium oxalate. Calcium analysis results are as follows: CaCl2 + COONH4 2 Ca COO 2 + 2NH4Cl The addition of ammonium oxalate solution causes the formation of a white precipitate such as fog +. The above reaction shows that the subject of kidney stones were used in this study contains calcium. leaves with lower levels, and whether Flavonoid qualitative analysis the dosage form of the extract has an effect on the solubility of calcium kidney stones. Kidney stones before it is treated with fragrant pandan extract, than it washed with distilled water flowing and dried. Afterwards, kidney stones crushed and then sieved with a sieve mesh size of 45. The purpose of the washing is done to remove impurities that exist in kidney stones. While sifting is done so that the size of the kidney stone that is used uniformly. To determine the content of calcium in the kidney stones were used then conducted a qualitative analysis of the chemical calcium. One purpose of this study was to determine the effect on the solubility of fragrant pandan extract calcium kidney stones in vitro, therefore, needed kidney stones contain calcium as one of the constituent components. 583 carried out by Wilstatter Test. This analysis aims to determine the content of flavonoids in pandan wangi leaves extract used in the study. Flavonoida are polar compounds that can form complexes with metal ions. Wilstatter In tests, the addition of magnesium as the metal ion. In literature, it is mentioned that the test is done by extracting the equivalent of 10 grams of plant material. and added hexane until colorless. The purpose of the addition of hexane is to attract chlorophyll that can interfere during the examination of flavonoids. Then, the hexane phase discarded, the residue mixed with 10 ml of 80 ethanol. This filtrate concentrated HCl plus 0.5, then added 3- 4 magnesium metal. Results can be seen after 10 minutes. From this test get a deep red color that identifies a class of flavonoid compounds flavonols. Quantitative analysis of the solubility of calcium kidney stones is done by adding 50 mg powder kidney stones each into 7 groups. The seventh group include: 5 ml of distilled water as a negative control, 5 ml calsusol 1.2 as positive control, and sample each 5 ml pandan wangi leaves extract 0.625, 5 ml pandan wangi leaves extract 1.25, 5 ml pandan wangi leaves extract 2.5, 5 ml pandan wangi leaves extract 5, and 5 ml pandan wangi leaves extract 10,. Soaking carried out for 5 hours at a temperature of ± 37 ° C and every minute is done shaking for 1 minute. After soaking for 5 hours, 7 groups filtered using filter paper. In this study, used Calcusol Standard curve of standard solution absorbance measurement results can be seen in Table 4. Before the measurement of absorbance, sample first made sample dilution. The sample dilution due to high calcium in the sample. Absorbance measurement results show the sample absorbance after being diluted. The concentration of dissolved calcium in the sample before dilution can be calculated by multiplying the dilution factor with the concentration of calcium measured. Atomic absorption spectrophotometer analysis results processed using descriptive statistical methods to determine whether a data Explore each sample has a normal distribution and positive control because it is a homogeneous variants. Explore normal traditional medicine that kidney stones proven efficacious and have been determined by a doctor. As for the negative control used is distilled water. The use of negative control aims to look at the possibility of dissolution of calcium for treatment during soaking and shaking. Selection of atomic absorption spectrophotometer is based on the ability to measure levels of metals with high accuracy and low detection limit. Atomic absorption spectrophotometer can detect calcium metal to levels of 0,002 ppm. Analysis of calcium in fragrant pandan extract made with flame atomization at a wavelength of 422.7 nm, air oxidant 15 l min, acetylene fuel 2 l min. A wavelength of 422.7 nm is the wavelength of maximum spectral calcium. A mixture of air-acetylene flame temperature required to reach about 2300 ° C. The high temperatures needed to melt, evaporate, then mengatomkan calcium metal which has a melting point of 838 ° C and a boiling point of 1440 ° C. Before measuring the distribution in statistics ditujukkan with the ratio between the value of skewness and kurtois and strandart error no more than ± 2. Variant data are expressed homogeneous when its significance is greater than 0.05. Explore the results of the analysis show that the data were normally distributed and had homogeneous variant. Analysis of the One Way ANOVA statistical method performed to see whether there are differences in the data from each sample and whether it is a significant difference or not. Methods One Way Anova been a parametric analysis method for data with one free variable that is first of the two treatments and unpaired. Calcium solubility data analysis using One Way ANOVA showed significance value less than 0.05 sig. 0.05. Sig. 0.05 indicates that there is a difference in the average value of the solubility of calcium kidney stones in distilled water negative control, positive control calcusol 1.2 and pandan wangi leaves extracts of each absorbance of the sample prior level. To find out which groups that measurement of the absorbance of standard solutions. Absorbance measurement standard solutions aimed at obtaining the raw curve equation used to calculate the level of calcium in the sample extract fragrant pandan leaves. 584 have an average difference of dissolved calcium, and if the difference is significant, the use of comparative analysis with the Tukey HSD method. In the analysis of Tukey HSD average significant difference between