Preparation of pandan wangi ethanol extract Treatment of kidney stones disease
NO Treatment
Result
1 Carbonat Assay
Kidney stone powder + HCl 10 CO
3
+ 2
Calcium Assay Kidney stone powder + 3 ml of
HCl 10, heated + NH4 oxalate saturation through the wall of the
tube do not shake A white precipitate is
formed like a fog +
3 Oksalat Assay
examination Sulfate Kidney stone powder + 1 ml of
HCl 10, boil + tip of a spoon MnO2
Arising gas +
4 Phospat Assay
idney stone powder + 3 ml of HCl 10, heated + NH4 oxalate
saturation through the wall of the tube do not shake
Pale yellow -
Sample Absorbance
Concent ration
ppm
Dilution factor
Concentration of sample ppm
Neg control.Aquades 0.0382
0.3813 10
3.813 Pos. control Calcusol 1.2
0.1162 1.4046
10 14.046
P.Wangi extract 0.625 0.1204
1.4597 10
14.597 P.Wangi extract 1.25
0.2258 2.8426
10 28.426
P.Wangi extract 2.5 0.2066
2.5907 50
129.535 P.Wangi extract 5
0.2335 2.9436
50 29.436
P.Wangi extract 10 0.4241
5.4442 50
272.21
Table 3. Results of the qualitative examination of calcium kidney stones
Table 4 Results of measurements of samples with SSA
Figure 2. Histogram test solutions versus the average Ca dissolved by atomic absorption spectrophotometer, the results of immersion calcium kidney
stones with the test solution for 5 hours
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RESULT AND ANALYSIS
Pandan wangi used in this study was obtained from Purwodadi. This
study begins with the identification of pandan wangi will be used. This step
aims to ensure that the plants used are not wrong and actually comes from
Pandanus amaryllifolius Roxb
species. The extraction method used in this
research was maceration with ethanol 70 as solvent. Ethanol are neutral, can
inhibit the growth of mold and bacteria, non-toxic, can be mixed with water at
any ratio, and the time required for a concentration of less Depkes, 1986. So
it is expected these solvents can obtain optimal active substances because it can
attract active substances that dissolve in water or in alcohol. The amount of 70
ethanol used in this maceration is 10 times the weight of crude drugs.
Pandan wangi leaves extract made with a series of levels of 0.625,
1.25, 2.5, 5 and 10, the selection is based on a series of levels of study
Rahardjo with levels of 10 proved to have an influence on the solubility of
calcium kidney stones. For further research then made a different dosage
forms that extracts fragrant pandan In this case, the researchers only
identify the elements which generally form kidney stones among other things
carbonate, calcium, oxalate, and phosphate. However, the most important
element in this study is calcium. For qualitative analysis of calcium, carried
out by first adding calcium powder with 10 HCl. The addition of 10 HCl
aims to dissolve the calcium. Selection of HCl 10 based on the theory that
most of the compounds in the form of calcium kidney stones of calcium
oxalate. This is evident in the analysis of oxalate which showed positive results.
Calcium oxalate is only soluble in strong mineral acid HCl 10 so selected that a
strong mineral acid as the solvent of calcium oxalate. After the addition of
10 HCL and heated, then the solution was added ammonium oxalate.
Calcium analysis results are as follows:
CaCl2 + COONH4 2 Ca COO 2 + 2NH4Cl
The addition of ammonium oxalate solution causes the formation of
a white precipitate such as fog +. The above reaction shows that the subject of
kidney stones were used in this study contains calcium.
leaves with lower levels, and whether Flavonoid qualitative
analysis the dosage form of the extract has an
effect on the solubility of calcium kidney stones.
Kidney stones before it is treated with fragrant pandan extract, than it
washed with distilled water flowing and dried. Afterwards, kidney stones crushed
and then sieved with a sieve mesh size of 45. The purpose of the washing is
done to remove impurities that exist in kidney stones. While sifting is done so
that the size of the kidney stone that is used uniformly. To determine the
content of calcium in the kidney stones were used then conducted a qualitative
analysis of the chemical calcium.
One purpose of this study was to determine the effect on the solubility of
fragrant pandan extract calcium kidney stones in vitro, therefore, needed kidney
stones contain calcium as one of the constituent components.
583 carried out by Wilstatter Test. This
analysis aims to determine the content of flavonoids in pandan wangi leaves
extract used in the study. Flavonoida are polar compounds that can form
complexes with metal ions. Wilstatter In tests, the addition of magnesium as the
metal ion. In literature, it is mentioned that the test is done by extracting the
equivalent of 10 grams of plant material. and added hexane until colorless.
The purpose of the addition of hexane is to attract chlorophyll that can
interfere during the examination of flavonoids. Then, the hexane phase
discarded, the residue mixed with 10 ml of 80 ethanol. This filtrate
concentrated HCl plus 0.5, then added 3- 4 magnesium metal. Results can be seen
after 10 minutes. From this test get a deep red color that identifies a class of
flavonoid compounds flavonols.