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The Activity Test of Pandan Wangi Leaves Extract pandanus amaryllifolius Roxb forSolubility of Calsium Kidney Stones by in Vitro Method using atomic Absorbtion Spectrophotometri AAS Ida Kristianingsih 1 , Dewi Resti Basuki 2 , Siti Mamluk Atul M 3 FAKULTAS FARMASI INSTITUT ILMU KESEHATAN BHAKTI WIYATA KEDIRI Email: id_kristyahoo.co.id Abstract Pandanus amarylliflius Roxb . folium has been used from generation to generation as kidney stones medicine. Mursito 2001 in his book said tha Pandanus amarylliflius Roxb folius has flavonoid as one of the constituents. In accordance with Pramono 1986, one of the kidney stones medicine mechanisme is by forming dissolvable calcium-flavonoid complex. The experiment was purposed to know whether water extract of Pandanus amarylliflius Roxb folium contains flavonoid and could dissolve calcium contained in kidney stones. Kidney stones as subject were homogenized its fitrated sefted using a mess 45, and then to test of qualitatively analized chemically. Pandanus amarylliflius extracted with water and get analized qualitatively with Wilstatter Test. The kidney stones divided into seven group, each of them soaked in aquadest, Calcusol 1,2, solution, Pandanus amarylliflius Roxb. Folium extract 0,625, 1,25, 2,5, 5 and 10,during ±5 hour with temperature ±37°C. Quantitative analize with Atomic Absorption Spectrophotometer was done to the solution after soaking. Qualitative analysis of kidney stones calcium shows positive results. Flavonoid qualitatif analysis with Wilstatter Test shows possitive results of flavonoid with carmine colours. Atomic Absorption Spectrophotometer data analysis by One Way Anova shows significant differentiation the amount of dissolved kidney stones calcium in aquadest negative control and 1,2 Calcusol as positive control are 0,3812 ± 0,0027 ppm and 1,4053 ± 0,0051 ppm; The0,625, 1,25, 2,5, 5 and 10, Pandanus folium extract could dissolved kidney stones calcium 1,4590 ± 0,0065 ppm, 2,8419 ± 0,0078 ppm, 2,5909 ± 0,0176 ppm, 2,9439 ± ,00740 ppm, 5,4445 ± 0,02562 ppm. The experiment results shows that water extract of Pandanus amarylliflius Roxb. ,. Leaves contains flavonoids and could dissolve calcium in kidney stones. The experiment results shows that water extract of Pandanus amarylliflius Roxb. , folium contains flavonoids and could dissolve calcium in kidney stones Keyword : Pandanus amarylliflius Roxb. , kidney stone, calcium, Atomic Absorption Spectrophotometer INTRODUCTION The people considers that the used of traditional medicine was safer than chemical medicine, so that they prefer to used traditional medicine to cure many diseases. They are usually consumed in the form of stew, steeping, or directly consuming that are considered nutritious Anonim, 2008. Kidney stones were one of the health problems in the urinary system, because the body is dehydrated, its 577 causing turbidity or urine becomes concentrated. The next result of blockage in the drain from the kidney to the bladder which can disrupt the smooth flow of urine. If the urine is obstructed exit of the kidney, there will be accumulated of urine in the kidney. In addition, it can also lead to infection due to the emergence of bacteria or viruses from the bladder. Accumulation of urine in the kidneys and infection that lasts a long time will lead to a decrease in the physiological function of the Method kidneys. 1. Processing of simplicia Traditional medicine used Pandan Wangi Leaves plants empirically is pandan wangi Pandanus amaryllifolius Roxb . it used by the community as a laxative medicine grow away from water sources and harvested during the dry season. The leaves are taken is growing 8 strands of kidney stones. The people used it shoots up to 8 strands of the base and hereditary and based on their than its were cut 10 cm from the base of experiences used boil water of pandan wangi leaves to treat kidney stones. One of the laxative mechanism drug by dissolving kidney stones are calcium kidney stones. Pandan wangi leaves, according Dalimartha 2000 contains flavonoids. In his research, Pramono 1986, states that the mechanism of dissolution of calcium kidney stones with the possibility of the formation of complexes between ionic calcium kidney stones with a hydroxy group carbonyl flavonoids contained in traditional medicine. Rahardjo study 2003 showed that infusion of pandan wangi leaves can dissolve calcium kidney stones. Some results phytochemical screening of pandan the leaf. Leaves were disorted and washed with flow of water. The leaves are allowed to dry with air flow and Its not exposed to direct sunlight.

2. Preparation of pandan wangi ethanol extract

Pandan Wangi Leaves were dried under shade and then were powdered with a sieve number 8 made extrac. Extraction using 170 g 10 parts simplicia pandan wangi leaves by maceration with ethanol 70 as much as 1.275 ml 75 parts. Maceration performed for 5 days with stirring twice a day. Maserat obtained from filtration was collected. The remaining pulp macerated again 2 days, filtered and wangi contains alkaloids, saponins, collected to obtain 1700 ml 100 parts. flavonoids, tannins, and a dye. These flavonoids can be extracted with ethanol 70. Ethanol can be used to sum up the relatively high polarity substance until relatively low. Maserat obtained evaporated with a rotary evaporator at a temperature of not more than 500C, until a thick consistency is formed future. The Extract is made by weighing 10 grams Pandan wangi leaves ethanol of pandan wangi leaves extract is extract is obtained through maceration which are a method that is suitable for compounds that do not withstand heating to high temperatures and is often dissolved in 100 ml of solvent DMSO and distilled water.

3. Treatment of kidney stones disease

used for extracting the ingredients which Kidney stones are made of floured smooth simplicia Voigt, 1994. Preparations of infusa can only sum up the substances that are polar, penyarian this way produce pollen that is unstable and easily contaminated by bacteria and fungi, therefore the juice obtained must powder by means of crushed using a mortar and sieved with a mesh size 45. After sifted powder kidney stones washed with distilled water and dried flows. not be stored for more than 24 hours. 4. Preparation of calcusol 1.2 a Another disadvantage is the cause swelling of the cell so that the active ingredient will be firmly attached to the bulbs. While the dosage forms in addition to the extract can be stored for longer also can be used repeatedly. 578 therapeutic dose of 1 day Calcusol as a positive control, six capsules calcusol 3 x daily therapeutic dose of 2 capsules is opened and its contents weighed 2.4 grams gained weight. The powder is then dissolved in distilled water to 200 ml volume one drink→ levels of 2.4 g 200 ml = 1.2