Preparation tool The results of the analysis of cutaneous Stimulus Effect Slow-

Quantitative analysis of the solubility of calcium kidney stones is done by adding 50 mg powder kidney stones each into 7 groups. The seventh group include: 5 ml of distilled water as a negative control, 5 ml calsusol 1.2 as positive control, and sample each 5 ml pandan wangi leaves extract 0.625, 5 ml pandan wangi leaves extract 1.25, 5 ml pandan wangi leaves extract 2.5, 5 ml pandan wangi leaves extract 5, and 5 ml pandan wangi leaves extract 10,. Soaking carried out for 5 hours at a temperature of ± 37 ° C and every minute is done shaking for 1 minute. After soaking for 5 hours, 7 groups filtered using filter paper. In this study, used Calcusol Standard curve of standard solution absorbance measurement results can be seen in Table 4. Before the measurement of absorbance, sample first made sample dilution. The sample dilution due to high calcium in the sample. Absorbance measurement results show the sample absorbance after being diluted. The concentration of dissolved calcium in the sample before dilution can be calculated by multiplying the dilution factor with the concentration of calcium measured. Atomic absorption spectrophotometer analysis results processed using descriptive statistical methods to determine whether a data Explore each sample has a normal distribution and positive control because it is a homogeneous variants. Explore normal traditional medicine that kidney stones proven efficacious and have been determined by a doctor. As for the negative control used is distilled water. The use of negative control aims to look at the possibility of dissolution of calcium for treatment during soaking and shaking. Selection of atomic absorption spectrophotometer is based on the ability to measure levels of metals with high accuracy and low detection limit. Atomic absorption spectrophotometer can detect calcium metal to levels of 0,002 ppm. Analysis of calcium in fragrant pandan extract made with flame atomization at a wavelength of 422.7 nm, air oxidant 15 l min, acetylene fuel 2 l min. A wavelength of 422.7 nm is the wavelength of maximum spectral calcium. A mixture of air-acetylene flame temperature required to reach about 2300 ° C. The high temperatures needed to melt, evaporate, then mengatomkan calcium metal which has a melting point of 838 ° C and a boiling point of 1440 ° C. Before measuring the distribution in statistics ditujukkan with the ratio between the value of skewness and kurtois and strandart error no more than ± 2. Variant data are expressed homogeneous when its significance is greater than 0.05. Explore the results of the analysis show that the data were normally distributed and had homogeneous variant. Analysis of the One Way ANOVA statistical method performed to see whether there are differences in the data from each sample and whether it is a significant difference or not. Methods One Way Anova been a parametric analysis method for data with one free variable that is first of the two treatments and unpaired. Calcium solubility data analysis using One Way ANOVA showed significance value less than 0.05 sig. 0.05. Sig. 0.05 indicates that there is a difference in the average value of the solubility of calcium kidney stones in distilled water negative control, positive control calcusol 1.2 and pandan wangi leaves extracts of each absorbance of the sample prior level. To find out which groups that measurement of the absorbance of standard solutions. Absorbance measurement standard solutions aimed at obtaining the raw curve equation used to calculate the level of calcium in the sample extract fragrant pandan leaves. 584 have an average difference of dissolved calcium, and if the difference is significant, the use of comparative analysis with the Tukey HSD method. In the analysis of Tukey HSD average significant difference between samples is shown with significance value of less than 0.05. Significance value obtained from the comparison of the average solubility of calcium each sample in Tukey HSD analysis is less than 0.05. it can be said that the average solubility of calcium in distilled water negative control, positive control Calcusol 1.2, and the pandan wangi leaves extracts from each of the different levels of meaning or difference is real or significant. It is marked on the Mean Difference column contained an asterisk . The amount of calcium dissolved in pandan wangi leaves extract and the percentage increase of the negative control and a positive control Calcusol distilled water of 1.2 can be seen in Table V. Comparison between the negative control and a positive control calcusol distilled water of 1.2 indicates that the ability to extract fragrant pandan leaves 2 , 5 calcium form soluble complexes with calcium so that an increasing number of flavonoids would increase the amount of dissolved calcium. Research pandan wangi leaves extract effect on the solubility of calcium kidney stones in vitro results that pandan wangi leaves extract content of 0.625 to 10 can dissolve calcium kidney stones. ability to dissolve calcium kidney stones caused by the flavonoids in pandan wangi leaves extract. This study is the first step to determine the ability of pandan wangi leaves extract kalsim in dissolving kidney stones. Further studies need to be performed in vivo to determine the ability of pandan wangi leaves extract in dissolving calcium kidney stones. CONCLUTION The experiment results shows that water kidney stones dissolve the lower extract of Pandanus amarylliflius 2.5909 and 84.3663. Aquadest comparison with the negative control and positive control Calcusol 1.2 indicated that the ability of pandan wangi leaves extract 0.625, 1.25, 5 and 10 are able to dissolve calcium kidney stones with each Roxb. ,. folium contains flavonoids and could dissolve calcium in kidney stones REFERENCES 1. Anonim. 2008. Sediaan Galenik . Jakarta: Departemen Kesehatan RI percentage increased, 282.6381 ± 2. Anonim. 1979. Vademekum 3.8265, ± 645.3675 102.2272, 672.1198 ± 109.4855, 287.4315 ± 1327.9916, Bahan Obat Alam . Jakarta: Depkes RI although a less capable but wandan 3. Anonim. 2008. Buku Pintar pandan wangi leaves extract 2.5 still has the potential to dissolve calcium kidney stones because Tukey HSD analysis results showed significant differences between calcium dissolved in a pandan wangi leaves extract 2.5. Histogram test solutions versus the average Ca dissolved by atomic absorption spectrophotometry, results immersion calcium kidney stones with the test solution for 5 hours. Histogram relationship between the test solution with the amount of dissolved calcium indicates that the higher the concentration of the extract, the amount of dissolved calcium also higher. Flavonoids contained in the extract to 585 Tanaman Obat . Jakarta: Agromedia, 16-17. 4. Cahyono. 2009. Bagaimana Cara Mencegah dan Mengatasi Batu Ginjal. yogyakarta 5. Dalimartha, Setiawan. 2000. Atlas Tumbuhan Obat Indonesia. Jilid I. Trubus Agriwidya. Jakarta. 103- 104 6. Gandasoebrata, R. 1968. Penuntun Laboratorium Klinik. Jilid I. Dian Rakyat. Jakarta. 186- 187 7. Gandjar, Ibnu, Gholib. 2007. Analisis Kimia Farmasi. Yogyakarta