Preparation tool The results of the analysis of cutaneous Stimulus Effect Slow-
Quantitative analysis of the solubility of calcium kidney stones is
done by adding 50 mg powder kidney stones each into 7 groups. The seventh
group include: 5 ml of distilled water as a negative control, 5 ml calsusol 1.2 as
positive control, and sample each 5 ml pandan wangi leaves extract 0.625, 5
ml pandan wangi leaves extract 1.25, 5 ml pandan wangi leaves extract 2.5,
5 ml pandan wangi leaves extract 5, and 5 ml pandan wangi leaves extract
10,. Soaking carried out for 5 hours at a temperature of ± 37 ° C and every
minute is done shaking for 1 minute. After soaking for 5 hours, 7 groups
filtered using filter paper.
In this study, used Calcusol Standard curve of standard solution
absorbance measurement results can be seen in Table 4. Before the measurement
of absorbance, sample first made sample dilution. The sample dilution due to high
calcium in the sample. Absorbance measurement results show the sample
absorbance after being diluted. The concentration of dissolved calcium in
the sample before dilution can be calculated by multiplying the dilution
factor with the concentration of calcium measured.
Atomic absorption spectrophotometer analysis results processed using
descriptive statistical methods to determine whether a data Explore each
sample has a normal distribution and
positive control because it is a
homogeneous variants. Explore normal traditional medicine that kidney stones
proven efficacious and have been determined by a doctor.
As for the negative control used is distilled water. The use of negative
control aims to look at the possibility of dissolution of calcium for treatment
during soaking and shaking. Selection of atomic absorption spectrophotometer is
based on the ability to measure levels of metals with high accuracy and low
detection limit. Atomic absorption spectrophotometer can detect calcium
metal to levels of 0,002 ppm. Analysis of calcium in fragrant pandan extract
made with flame atomization at a wavelength of 422.7 nm, air oxidant 15 l
min, acetylene fuel 2 l min.
A wavelength of 422.7 nm is the wavelength of maximum spectral
calcium. A mixture of air-acetylene flame temperature required to reach
about 2300 ° C. The high temperatures needed to melt, evaporate, then
mengatomkan calcium metal which has a melting point of 838 ° C and a boiling
point of 1440 ° C. Before measuring the distribution in statistics ditujukkan with
the ratio between the value of skewness and kurtois and strandart error no more
than ± 2. Variant data are expressed homogeneous when its significance is
greater than 0.05. Explore the results of the analysis show that the data were
normally distributed and had homogeneous variant.
Analysis of the One Way ANOVA statistical method performed to
see whether there are differences in the data from each sample and whether it is
a significant difference or not. Methods One Way Anova been a parametric
analysis method for data with one free variable that is first of the two
treatments and unpaired.
Calcium solubility data analysis using One Way ANOVA showed
significance value less than 0.05 sig. 0.05. Sig. 0.05 indicates that there
is a difference in the average value of the solubility of calcium kidney stones
in distilled water negative control, positive control calcusol 1.2 and
pandan wangi leaves extracts of each
absorbance of the sample prior
level. To find out which groups that measurement of the absorbance of
standard solutions. Absorbance measurement standard solutions aimed
at obtaining the raw curve equation used to calculate the level of calcium in the
sample extract fragrant pandan leaves.
584 have an average difference of dissolved
calcium, and if the difference is significant, the use of comparative
analysis with the Tukey HSD method.
In the analysis of Tukey HSD average significant difference between
samples is shown with significance value of less than 0.05. Significance
value obtained from the comparison of the average solubility of calcium each
sample in Tukey HSD analysis is less than 0.05. it can be said that the average
solubility of calcium in distilled water negative control, positive control
Calcusol 1.2, and the pandan wangi leaves extracts from each of the different
levels of meaning or difference is real or significant. It is marked on the Mean
Difference column contained an asterisk .
The amount of calcium dissolved in pandan wangi leaves extract and the
percentage increase of the negative control and a positive control Calcusol
distilled water of 1.2 can be seen in Table V. Comparison between the
negative control and a positive control calcusol distilled water of 1.2
indicates that the ability to extract fragrant pandan leaves 2 , 5 calcium
form soluble complexes with calcium so that an increasing number of flavonoids
would increase the amount of dissolved calcium.
Research pandan wangi leaves extract effect on the solubility of
calcium kidney stones in vitro results that pandan wangi leaves extract content
of 0.625 to 10 can dissolve calcium kidney stones. ability to dissolve
calcium kidney stones caused by the flavonoids in pandan wangi leaves
extract.
This study is the first step to determine the ability of pandan wangi
leaves extract kalsim in dissolving kidney stones. Further studies need to be
performed in vivo to determine the ability of pandan wangi leaves extract in
dissolving calcium kidney stones.
CONCLUTION The experiment results shows that water
kidney stones dissolve the lower extract of
Pandanus amarylliflius
2.5909 and 84.3663. Aquadest comparison with the
negative control and positive control Calcusol 1.2 indicated that the ability
of pandan wangi leaves extract 0.625, 1.25, 5 and 10 are able to dissolve
calcium kidney stones with each Roxb.
,.
folium contains flavonoids and could dissolve calcium in kidney stones
REFERENCES
1. Anonim. 2008.
Sediaan Galenik
. Jakarta: Departemen Kesehatan
RI percentage increased,
282.6381 ± 2. Anonim. 1979.
Vademekum
3.8265, ± 645.3675 102.2272, 672.1198 ± 109.4855, 287.4315 ± 1327.9916,
Bahan Obat Alam
. Jakarta: Depkes RI
although a less capable but wandan 3. Anonim. 2008.
Buku Pintar
pandan wangi leaves extract 2.5 still has the potential to dissolve calcium
kidney stones because Tukey HSD analysis results showed significant
differences between calcium dissolved in a pandan wangi leaves extract 2.5.
Histogram test solutions versus the average Ca dissolved by atomic
absorption spectrophotometry, results immersion calcium kidney stones with
the test solution for 5 hours. Histogram relationship between the test solution
with the amount of dissolved calcium indicates that the higher the
concentration of the extract, the amount of dissolved calcium also higher.
Flavonoids contained in the extract to
585
Tanaman Obat
. Jakarta: Agromedia, 16-17.
4. Cahyono. 2009. Bagaimana Cara Mencegah dan Mengatasi Batu
Ginjal. yogyakarta 5. Dalimartha, Setiawan. 2000. Atlas
Tumbuhan Obat Indonesia. Jilid I. Trubus Agriwidya. Jakarta. 103-
104 6. Gandasoebrata, R. 1968.
Penuntun Laboratorium Klinik. Jilid I. Dian Rakyat. Jakarta. 186-
187 7. Gandjar, Ibnu, Gholib. 2007.
Analisis Kimia Farmasi. Yogyakarta