Gambar 3.7 Variabilitas kelapa Kopyor Genjah Pati A dan B dan kelapa Kopyor Dalam Jember C and D berdasarkan duplex PCR dari dua lokus marka
SNAP. Gambar A dan C menggunakan primer WRKY61Ref 107 pb dan WRKY22Ref 260 pb, B dan D menggunakan primer
WRKY61Alt 107 pb dan WRKY22Alt 260 pb. No.1-15: nomor sampel kelapa Kopyor. M: 100 pb DNA ladder
3.4 Simpulan
Berdasarkan 35 data sekuens gen WRKY di GenBank NCBI, dapat mendisain 8 primer SNAP gen WRKY untuk gen WRKY2, WRKY6, WRKY7,
WRKY19 dan WRKY21. Kedelapan primer berhasil diuji dengan reaksi singleplex PCR, yang hasilnya menjadi acuan untuk validasi reaksi multiplex
PCR. Reaksi multiplex PCR yang berhasil diperoleh adalah reaksi duplex PCR dan telah diaplikasikan untuk kelapa kopyor Pati dan Jember.
1000 pb 500
100
M 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 M 1 2 3 4 5 6 7 8 9 10 11
260 pb 107
1000 pb 500
100
1000 pb 500
100 1000 pb
500
100
260 pb 107
260 pb 107
260 pb 107
A B
C D
Ref Alt
Ref Alt
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