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Sample Preparation and Extraction. The red dragon fruit peel Hylocereus

274 Figure 1. Standard curves of quercetin at total flavonoids test f the absorbance value of the RDFP‐AcOEt fraction put in the y‐axis in the quercetin linear regression equation then the levels of flavonoids in the sample x‐axis can be known. The result shows that the average levels of flavonoids in the sample RDFP‐AcOEt fractions was .8 ± . ppm. So that the levels of total flavonoids from RDFP‐AcOEt fractions obtained from the calculation was . ± . ww EQ Table . t means that every grams of KBNM‐AcOEt fractions equivalent to . grams of flavonoids quercetin. When compared with the research of Chet , RDFP‐AcOEt fractions showed higher levels of flavonoids than the water extracts of RDFP with an average grade of flavonoid about .8 ± , mg catechin equivalents g. Table 2. Total Flavonoid Content in RDFP‐AcOEt Fraction Replication Absorbance Flavonoid Level in Sampel ppm Total Flavonoid bb EQ 1 . . . 2 . .8 . 3 . . . Average .8 . Deviation Standard . .

3.3. Total Phenolic Content. Total phenolic content of the RDFP‐AcOEt fraction

done using the Folin‐Ciocalteu method. Gallic Acid Equivalent GAE is a common reference for measuring the amount of phenolic compounds present in a material. Usage of gallic acid was based on the availability of the substance is more stable and pure than the other standards compounds. The relationship between the absorbance values and the concentrations of gallic acid would produce a linear regression equation. Gallic acid linear regression equation obtained was y = , x + . with the value of R² = . 8 Figure . Total phenolic content of the RDFP‐AcOEt fraction x‐axis is calculated by entering the absorbance value of the sample of RDFP‐AcOEt fractions into the y‐axis. The result shows an average of total phenol content of the RDFP‐AcOEt fraction C value was . ± . mg GAEmL. The dilution is not done in this test and the stock solution used was . mg RDFP‐ AcOEt fractions were dissolved in mL ethanol. Therefore, the average of total phenolic content TPC of RDFP‐AcOEt fractions obtained from the calculation was . ± . mg GAE g Table . t means that every grams of RDFP‐ AcOEt fractions equivalent to . mg gallic acid. When compared with the research of Chet , RDFP‐AcOEt fractions showed total phenol content higher than RDFP water extracts with an average of total phenol content of . ± .8 mg GAE g. y = 0.0009 ‐ 0.1414 R² = 0.9904 0. 1 1. 2 00 1000 1 00 2000 2 00 Ab sorbance Concentration μgml

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