Introduction Material and Methods
3. Result and Discussion
The observation of alveolar histology are presented in tables and pictures below : Table . The average thickness of the septum interalveolaris Group Average m± Standard Deviation Control K , ± , 88 a Gel P 8, ± , 8 8 b Spray P , ± , c a, b, c = shows significant differences with statistical test Mann Whitney Table . The average diameter of the alveoli Group Average µ ± Standard Deviation Control K , ± , 8 a Gel P , 8 ± , b Spray P , ± , 8 c a, b, c = showed statistically significant differences with the Mann Whitney test Table . The number of types of inflammatory cells No nflammatory Average Number of cells ± SD Control K Gel P Spray P Lymphocytes . ± , a . ± , b . ± , c PMN . ± , 8 d . ± , 8 e . ± , e Plasma . ± ,88 f .8 ± , g . ± , h Eosinophils . ± , i . ± , j . ± , j istiocytes . ± , k . ± , l . ± , l a‐i = show statistically significant differences with the Mann Whitney test a b c Figure 1. a Alveolar histology of Control group K HE, 10x10; b Alveolar histology of Gel group P1 HE, 10x10; c Alveolar histology of Spray group P2 HE, 10x10 197 This study shows that the effects of exposure to the gel air freshener septum thickness interalveolaris worse than freshener spray. Freshener gels, contain more formaldehyde than the liquid preparation spray SCER, . Mechanisms interalveolaris septal thickening gel group P caused by formaldehyde inhalation causes edema in the tissue around the pulmonary alveoli due to inflammation that occurs through the stimulation of tachykinin NK receptors and oxidative stress mechanisms. n the spray group P , interalveolaris septal thickening caused by di‐ ‐ ethylhexyl phthalate DEP phthalate derivatives which are compounds that are found in room deodorizers liquid spray . Compounds that settles in pulmonary DEP can act as a partial agonist of the Peroxisome Proliferator‐Activated Receptor‐γ PPARγ and stimulate oxidative stress. Both of these mechanisms cause edema septum interalveolaris therefore inflammatory process Kocbach, A. . Septal thickening interalveolaris also affected by the process of atelectasis lung development is not perfect due to alveolar collapse . n this study, atelectasis caused by lung extrinsic pressure increased due to scar tissue fibrosis of the septum interalveolaris Price, S and Wilson, L. M., . As a result of the collapse of the alveoli, the septum adjacent interalveolaris will blend and conjured images of a thickened septum. The results showed that the average diameter of alveolar spray group control group group gel. Alveolar diameter size changes in the gel group, influenced by the effects of formaldehyde in air freshener gels which causes edema and atelectasis. Both processes spur interalveolaris septal thickening, so that will compress the size of the alveoli Mohamed, A. M. T., et al , . This makes the size of the diameter of the alveoli become increasingly narrow. This study has shown that alveolar diameter in the spray group S , has the widest measure than other groups. This happens because of their exposure to DEP compound that causes increased production of free radicals Reactive Oxygen Substance ROS You,., et al., . Free radicals can damage cell membranes and protein constituent spur proteolytic degradation of elastin by elastase. Resulting in a drop forming septum interalveolaris woven from fibers elastin and reticuline Anindyajati, EA., . Acute inflammatory cells, appears to dominate the control group. Mild acute inflammation that occurs in the control group, may be caused by inhalation of ammonia substances in animal urine test and not caused by exposure freshener. The mechanism of injury occurs when ammonia gas N reacts with tissue fluids to form a highly alkaline solution, called ammonium hydroxide Permata, G. S., . Acute inflammatory cell infiltration in the gel group P and spray P is not only caused by the substance of ammonia, but also because their mechanism of acute exacerbation of chronic inflammation due to exposure freshener. Acute exacerbation of chronic inflammatory process causing no significant differences between groups of acute inflammatory cells, gel and spray. owever, the number of acute inflammatory cells and spray gel group more and has had a statistically significant difference with the control group. Gel group P and spray P is dominated by chronic inflammatory cells that should be marked by infiltration of lymphocytes, macrophages, and plasma cells are prominent. owever, in this study the cell histiocytes macrophages and spray gel group has an average amount of at least compared with other inflammatory cells. The condition occurs as a result of this study using formalin instead of formalin buffer for organParts
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