The Test of Antioxidant Potential with DPPH Method. Antioxidant potential
3.5. Measurement of Maximum Wavelength and SPF Sun Protection Factor
value. Measurement of in vitro SPF value is done by determining the wavelength of maximum absorption ‐max by spectrophotometric method. UV‐Vis spectrophotometer scanning performed at wavelengths between ‐ nm to determine the maximum wavelength of RDFP‐AcOEt fractions at each concentration into the range of UVA, UVB or UVC. The maximum wavelength KBNM‐AcOEt fractions at concentrations of , , and ug mL known to reside in the UVB range area ‐ nm , while the maximum wavelength KBNM‐AcOEt fractions at a concentration of pg mL in the UVC range region ‐ nm Table . The concentration of RDFP‐AcOEt fractions which were in the UVB range can be calculated the SPF value using a formula that has been developed by Mansur et al. 8 . While the concentration of RDFP‐AcOEt fractions which were in the UVC range is not necessary SPF value calculation because the UVC rays are radiation which is not up to the earths surface being absorbed by the ozone layer McKinlay, 8 . The average value of the SPF in the UVB area of RDFP‐AcOEt fraction was . ± . . According to Wasitaadmatdja , the minimum SPF value of a sunscreen or photoprotective agent is at least . Therefore, it can be said that the RDFP‐AcOEt fraction concentration of , , and mgL does not have photoprotective potential. The low value of SPF on RDFP‐AcOEt fraction suspected to be caused by the concentration of RDFP‐AcOEt fractions used is too y = 0.0933x + 4.1018 R² = 0.9904 10 20 30 40 50 60 100 200 300 400 500 600 in hibit io n Concentration μgml 277 low. According to Widyastuti et al. , the concentration of ethanolic extract of super red dragon fruit peel ylocereus costaricensis FAC Weber Britton Rose demonstrated effectiveness either as a sunscreen at a concentration of ppm with SPF value of . 8, while at a concentration of ppm of ethanolic extract of super red dragon fruit peel does not show its effectiveness as sunscreen since the SPF value of less than or equal . . Table 5. The scanning of Maximum Wavelength of RDFP‐AcOEt Fraction Concentration µgmL The maximum Wavelength nm Absorbance Ultraviolet Area 5 . B 25 . 8 B 50 . B 100 . C Conclusion Ethylacetate fraction of an ethanolic extract of red dragon fruit peel RDFP‐AcOEt containing flavonoids at . ± . ww EQ is measured by the AlCl chelation method and total phenolic content of . ± . mg GAE g which measured by the Folin‐Ciocalteu method. Antioxidant potential of RDFP‐AcOEt fractions is weak by the C value that is equal to . µgmL measured by DPP method. SPF value KBNM‐AcOEt fraction was . ± . . This indicates KBNM‐AcOEt fraction concentration of , , and mgL were tested using in vitro methods spectrophotometry does not have significant photoprotective potential. Acknowledgements We thank nstitute of Research, Publications and Community Service of Universitas Muhammadiyah Yogyakarta for financial support through the Tridharma enhancement grant ‐ . References Abdul Rohman, Riyanto, S., idayati, N., , Antioxidant Activity, Total Phenolic Content and Leaf Total Flavonoids Noni Morinda citrifoia L , [Aktivitas Antioksidan, Kandungan Fenolik Total, dan Flavonoid Total Daun Mengkudu Morinda citrifoia L ], Agritech, Vol. No. . Budilaksono, W., Wahdaningsih, S., Fahrurroji, A., , Antioxidant Activity Test of N‐exane fraction Red Dragon Fruit Peel ylocereus lemairei Britton and Rose Method Using DPP , ‐ Diphenyl ‐ ‐ picrylhydrazyl , Journal of Pharmacy Students Faculty of Medicine UNTAN, [Uji Aktivitas Antioksidan Fraksi N‐eksana Kulit Buah Naga Merah Hylocereus lemairei Britton dan Rose Menggunakan Metode DPP , – Difenil – ‐ Pikrilhidrazil , Jurnal Mahasiswa Farmasi Fakultas Kedokteran UNTA]N, vol. no. . Chaiwut, P., O‐ki‐la, A., Phuttisatien, ., Thitilertdecha, N., Pintathong, P., , Extraction and Stability of Cosmetic Bioactive Compounds from Dragon Fruit Peel, Conference , School of Cosmetic Science Mae Fah Luang University, Thailand. Chet, N.W., , Total Phenolic and Total Flavonoids Content of Pitaya Peels by Water Extraction, Thesis, Faculty of Chemical and Natural Resources Engineering, Universiti Malaysia Pahang. Desmiaty, Y., Ratnawati, J., Andini, P., , penentuan Jumlah Flavonoid Total Ekstrak Etanol Daun Buah Merah Pandanus Conoideus Lamk Secara KolorimetriParts
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