METHOD 1 Time and location

Bogor, 21-22 October 2015 571 beekeepers to increase their income by doing diversification. According to Elton and Shimanuki 1978, bee bread is bee`s food produced in male flowers which was taken and stored in the honey bee nests by workers bee. Before the pollen is stored in the nest, it is added and mixed using saliva of worker bees Carpeset. al., 2007, nectar Somerville, 2007, lactic acid bacteria Egorova, 1971 and little propolis Dennis, et. al., 2009. The existance of lactic acid bacteria makes it better and hashigher nutritional value compared to the origin pollen that is not treated by the bees Purnomo et. al, 2008. In Riau province, wildforest honey is most popular than honey in which produce by A. mellifera beekeeping. Purnomo et. al. 2008 stated that forest honey productivity in Riau is the highest in Indonesia. However, diversification products such as bee bread are needed. Today, bee bread has not been taken or ignored by the forest honey beekeepers due to the lack of sufficient information about the bee bread. As a result, they assume that bee bread is honey bee feces and it should be discarded and abandoned when honey is harvested. Actually, bee bread is not only producing by A. dorsata in the forest but also making by T. itama. stingless bee. Similar to the A. dorsata, many beekeepers that keeping this stingless bee also think that bee bread is useless because they only want to gain the honey. Products such as bee pollen have been widely commercialized in the form of capsules and are sold in pharmacies as a dietary supplement for humans. However, the majority of this bee pollen product is derived A. mellifera Dinas Perkebunan ProvinsiJawaTimur, 2011 and until today we can not find the products using A. dorsata`s and T. itama`s bee bread. Whereas, the potency of A. dorsata bee bread is relatively abundant and unused. According to Purnomo et. al. 2008, the potency of A. dorsata`s bee bread in Riau province reached 50 to 100 tons per year and has not been utilized yet. In fact, it can be used as a feed supplement for A.mellifera, especially when the A. mellifera`s colonies is in poor conditions lack of pollen. For example, in the situation of lack of nectar, beekeepers can anticipate it by giving the syrup in the ratio 1: 1 Somerville, 2007. Yet as it turns out, it is more expensive for beekeepers to give their A. mellifera`s colonies the additionalsubsitute pollen instead of nectar substitute. By this condition the beekeepers used to use a fermented mixture of soy flour and milk Kuntadi, 2008. Therefore, there is need information about the composition of the A. dorsata and T. itama bee bread and to determine the effect A. dorsata`s bee bread to the A. mellifera colonies. The objectives of this research are to deliver the information about composition of A. dorsata`s and T. itama`s bee bread and to figure out the effect A. dorsata bee bread to the A. mellifera colonies. 2. METHOD 2.1 Time and location This research was conducted in two years 2010-2011 at 3 places in Riau. The first location where the sample of A. dorsata bee bread was taken located at Sungai Pagar village Kampar district. Perawang was the second location where the treatment was given to A. mellifera. Perawang is concession area of industrial plantation forest that was planted by Acacia sp. The third location where the analysis of amino acid was done was conducted at Center of Forest Products in Bogor. 2.2 Research design The research was used combination between field experiment and laboratorial activity. In the field experiment, colonies of A. mellifera was treated by using A. dorsata bee bread. The t-test was used to find out which A. mellifera colonies that gave significant respond regarding to the given of A. dorsata bee bread. Meanwhile, the value of protein and amino acid were done to A. Bogor, 21-22 October 2015 572 dorsata and T. itama bee bread. The sample of A. dorsata bee bread was taken from 3 sialang trees in Sungai Pagar village. Sample of T. itama bee bread was picked up from Riau province Kampar district and West Sumatera Tanah Datar district. 2.3 Observed parameter a. Composition of A. dorsata`s bee bread proteins, lipids, fibre, ash, carbohydrate, and amino acids. b. Composition of T. itama`s bee bread amino acids. c. Phytochemical component of A. dorsata`s and T. itama`s bee bread qualitative. d. Crude protein of A. mellifera workers. e. Honey productivity of A. mellifera. 2.4 Data Analysis Analysis of variance was used to know the effect of A. dorsata`s bee bread to the crude protein of A. mellifera colonies and honey productivity. Value is given as mean average. Meanwhile, the data of composition of bee bread A. dorsata and T. itama that were collected were analyzed by qualitative. A. dorsata bee bread was qualitatively compared to A, mellifera`s bee pollen and T. itama`s bee pollen. In addition, the protein composition of A. dorsata`s and T. itama`s bee bread were also compared to other pollens from different plants and food products. Moreover, the phytochemical analysist was also qualitatively conducted to find the products in which has better phytochemical compound alkaloid, steroid, flavonoid, tanin, and saponin. 3. RESULT AND DISCUSSION 3.1