MATERIAL AND METHOD 1 Fungal isolates RESULT AND DISCUSSION
Bogor, 21-22 October 2015
799 plants from pests and diseases Mejía et al., 2008; Vega et al., 2008, and to increase the
resistance of plants to various types of stress Lewis, 2004. Fungal endophytes have several mechanisms to promote plant growth, such as production of
plant growth regulating hormones and solubilization of insoluble minerals. Some fungal endophytes including Aspergillus flavus, A. niger, Fusarium oxysporum, Penicillium corylophilum, P.
cyclopium, P. funiculosum and Rhizopus stolonifer isolated from diverse kinds of plants were reported for their ability to produce different kinds of plant growth promoting hormones like indole
acetic acid IAA and gibberellic acid GA Hassan, 2002; Waqas et al., 2012: Nath et al., 2015. Various studies of endophytes application has been widely carried out in agricultural
crops, including tomatoes Zehnder et al., 1999; Schuhegger et al., 2006; Khallal, 2007, tobacco Zhang et al. 2002, wheat Gorlach et al., 1996 and rice De Vleesschauwer et al.,
2010. However, study for its application for forest crops are still rare. In this study, various isolates of fungal endophyte were analyzed to know their effect to the sengon Falcataria
moluccana seedling growth.
2. MATERIAL AND METHOD 2.1 Fungal isolates
Isolate of fungal endophytes used in this study were Trichoderma spp. such as isolate STB207, STK2012, STB208, STK3106 and STB107. This fungus is a collection of Laboratorium of
Forest Protection, The Centre for Forest Reseach and Development, Bogor. 2.2 Fungal endophytes production
Mass production of fungal endophytes were carried out by using rice media. Before being used for mass production, fungal endophytes were cultured in potato dextrose agar PDA
media for two weeks. The mycelia then harvested by adding sterilized water and then inoculated to the rice media. The rice media then incubated for 1 month before ready to be
used Saharayaj Namasivayam, 2008. 2.3 Application of fungal endophytes
Fungal endophyte were applied on 1 month seedling of sengon. Application were carried out by adding fungal endophyte under the plant root. About 100 g of mycelium was added in a
base of pot and then cover with soil media about 5 mm before planting the seedling in pot. 2.4 Analysis of sengon growth response by fungal endophyte application
The study was conducted using a completely randomized design CRD, which consists of seven levels i.e. control with no fertilizer, control + Sp36 0.2 g, Control + Sp36 0.4 g, isolate
STB207, STK2012, STB208, STK3106 and STB107. Each treatment had five replications. The parameters observed were the increase of plant height. Observations were carried out every
two weeks until sixth week. After final observation, the dry weight of root, stem and leave were measured.