Axillary buds initiation in F. moluccana micro propagation

Bogor, 21-22 October 2015 776

3.2 Axillary buds initiation in F. moluccana micro propagation

Gall rust tolerant F. moluccana shoots initiation started from axillary buds after 1 week inoculation on MS solid medium supplemented with varying level of BAP. This initiation media have good respond on sprouting ability for all F. moluccana families as explants source. In micro propagation, cytokinins include BAP are applied to promote cell division of axillary branching. After 3 times subculture in 3 months incubation, explants shoot have 3 kind regenerated sprouting, multi sprouting more than 6 shoots, medium sprouting 2-4 shoots and single sprouting Figure 2. The ANOVA test appropriate for the design was carried out to detect the significance of differences P0.05 among the treatment means Table 1. Table 1: Resume of ANOVA test results for shoot number per nodule and shoots height of 10 families of F. moluccana branches rejuvenation Source of Variation Degree of Freedom Sum of Squares Mean of Square F Sig. shoot number Between Groups 9 76.800 8.533 4.655 0.002 Within Groups 20 36.667 1.833 Total 29 113.467 shoot height Between Groups 9 423.200 47.022 1.354 0.272 Within Groups 20 694.593 34.730 Total 29 1117.794 Significant at 95 confident interval level a b c Figure 2: Axillary buds regeneration in F. moluccana micro propagation with multi a, medium b and single c sprouting ability after 3 months subcultures After 6 months subculture, the best result of mean shoot number in micro propagation was 10.00 ± 0.4472 from 0.5 mgl IBA concentration, obtain in family 8. The best result of mean shoot length was 6.20 cm ± 0.0471 from family 2 and 6.20 cm ± 0.0422 from family 9, both were from 0.5 mgl BAP concentration Table 4. Mean of shoot number and mean of shoot length values have not significant differences among 10 F. moluccana families, but in BAP treatments. Bogor, 21-22 October 2015 777 Table 2: Shoot number per nodule and shoots height of 10 families of F. moluccana branches rejuvenation for 1 month incubation in nursery Factor Mean of shoot number nodule Mean of shoot height cmnodule Family 1 2.6667 ± 0.3333 b 19.2000 ± 4.5177 b Family 2 3.3333 ± 0.6666 b 14.3000 ± 3.6116 a Family 3 3.0000 ± 1.0000 c 26.5000 ± 3.2716 c Family 4 3.6667 ± 1.4530 b 25.6667 ± 4.2463 b Family 5 3.0000 ± 0.0000 b 21.1000 ± 5.4745 b Family 6 1.3333 ± 0.3333 a 24.4000 ± 0.9504 b Family 7 4.3333 ± 08819 c 19.6667 ± 0.6333 b Family 8 7.6667 ± 0.8819 d 17.5667 ± 3.7391 b Family 9 4.0000 ± 0.0001 c 19.3333 ± 0.5487