METHODOLOGY 1 Preparation Prosiding INAFOR III 2015 FullIssue low rest

Bogor, 21-22 October 2015 400 Until now there have been a lot of research on bio-active compounds derived from plants as a natural insecticide mosquito dengue example of volatile components such as chemical plants, alkaloids, saponin, and quinone Mulyana 2002, Cheng et al., 2003, Chapagain et al. 2007 . Quinone effective as insecticide dengue mosquito larvae is 2-methyl-anthraquinone found in Sµgi Tree or Cryptomeria japonica Cheng et al. 2008 and anthraquinon contained in Cassia sp Yang et al. 2003. While the structure of 2-methyl-anthraquinone is a major component of the extractive teakwood Tectona grandis, which is known as tectoquinone Ohi 2001, Haupt et al. 2003. It has been reported that this tectoquinone responsible for teak natural durability Lukmandaru and Takashi 2008, 2009, but there are no studies on the use of rosewood extract as Aedes aegypti mosquito larvicides. 2. METHODOLOGY 2.1 Preparation The wood used in this study is teak T. grandis 45 years old . Teak wood comes from KPH Madiun, East Java. Mosquito larvae used were larvae of the dengue mosquito A. aegypti instar-IV. Larvae from the eggs of mosquitoes in 8 days. Chemicals used as solvent is ethanol and toluene. As the solvent extract before water is dissolved into dimethyl sulfoxide DMSO. Tools necessary for research that Willey mill, filter 10-100 mesh, vacuum evaporator, tools soxhletation, instrument of GC-MS Pyrolisis, petri disk, pipette, and oven. The form test sample 40-60 mesh powder prepared from teak T. grandis. 2.2 Extraction and isolation Teak wood extraction is prepared by the soxhletation method and separation of the solvent using a rotary vacuum evaporator. Extraction using ethanol and ethanol-toluene mixture with a ratio of 1: 1, 1: 2, 2: 1, and 3: 1. Concentration of the extract is calculated as weight percent of the dry sample weight. 2.3 Quantification levels tectoquinone Tectoquinone levels tested using Gas Chromatography Mass Pyrolisis spectrophotometer Pyr-GC-MS with test conditions of 400 C temperature pyrolysis, pyrolysis time of 1 hour, the temperature pyrolyzer and transfer tube 280 C, 280 C injection temperature, relative detector temperature, and the temperature of the column enhanced early 50 C 15 C min until the temperature reached 280 C. Under this test will get the type of solvent or solvent mixture is capable of isolating the most effective extract with tectoquinone high levels. The resulting extract from the solvent or solvent mixture highest will be used for testing its effectiveness as larvicides A. aegypti mosquito larvae. 2.4 Bioassay test Testing the effectiveness of larvicides extract teak refers to research conducted by Cheng et al. 2008. Ten larvae of A. aegypti dengue mosquito- 4th instar were placed in 24.5 ml of distilled water, followed by the addition of 500 microliters of DMSO solution containing the test sample in a glass beaker. The solution was shaken slowly so homogeneously mixed and left at room temperature. Bio-active concentration used is equivalent to 1.0; 2.5; 5.0; 7.5; 10; 12.5; and 15 µg ml tectoquinone levels. The controls are used in the form of 24.5 ml of distilled water and 500 microliters of DMSO. As a positive control used insecticide Abate with Temephos 1 active ingredient is the same as the equivalent concentration of extract treatment of teak. Larvicidal activity were evaluated for 24 hours after treatment is expressed as mosquito larvae mortality. The percentage of mortality corrected with the control. Toxic value measured by Bogor, 21-22 October 2015 401 15.99 15.45 18.67 16.72 16.79 2 4 6 8 10 12 14 16 18 20 E x tr a ct iv e C o n te n t Solvent the LC 50 and LC 90 value that indicates the number concentration in µg ml which causes 50 and 90 mortality mosquito larvae within 24 hours. 2.5 Data analysis The relative levels of 2-methyl-anthraquinone obtained directly after testing Pyr-GC-MS. Correlation between mortality with concentrations of 2-methyl-anthraquinone is determined by a simple regression using Microsoft Excel 2007. Determination of the lethal concentration 50 LC 50 and lethal concentration 90 LC 90 effect on the mortality of larvae of the dengue mosquito A. aegypti instra-IV with probit analysis method using the software Minitab 14 for Windows. 3. RESULT AND DISCUSSION 3.1 Extract levels