EXPERIMENTAL METHOD 1 Place and time research

Bogor, 21-22 October 2015 819 A study reported by Saptono 1988 mentioned that the increase in the number of seeds planted soybeans can be used to overcome a decline in production due to decreasing of seed vigor up to 63 along the optimum environmental conditions. The density of seeds that are sown in beds or trays sown very closely related to the vigor seedlings which were produced. The seed germinability in the soil as a seed testing in sub-optimum conditions that aimed at predicting the ability of the seeds to grow in the conditions close to the actual conditions in the field, with observations on the initial count and the final Kartika, 1994. Seed density provides information on the success rate of germination to support the procurement of seedling for the planting program. The effectiveness of using seeds for seedling transfer to the nursery container can be improved by determining how many seed needed for seed sowing on the seed beds. The purpose of this study was to determine the seed density of jelutung in order to maintain the seed spacing in the seed bed for producing vigour seedlings to support planting material in the field. 2. EXPERIMENTAL METHOD 2.1 Place and time research Seed collection of jelutung was conducted in Banyuasin, South Sumatra. Seed testing carried out in the laboratory of Seed Technology Research Institute of Forest Plants in Bogor. The study was conducted from April to July, 2014. 2.2 Materials and equipment The jelutung seed was used in this study, and other materials and equipment were plastic boxes, distilled water, alcohol, a medium mixture of soil and sand 1 : 1 vv, germination box size 20 cm x 30 cm and stationery.

2.3 Metodology Stages of the activities carried out in this study are as follows:

a The amount of seed required for each treatment was 200 seeds 4 replicates 50 seeds. The treatment consisted of 5 seed aging level, including 0 hours, 48 hours, 96 hours, 144 hours and 192 hours. b Seed aging was conducted by placing seeds on a wire gauze and put in a plastic container filled with water 150 ml, and keep the water not to watering the seeds. The containers are then placed in an incubator at a temperature of 40 ◦C. The seed was germinated by sowing the seed on the medium of soil and sand mixture 1: 1 vv in the tub sprouts. The calculation of seed germination was carried out at each level of seed aging. c Observations germination was conducted every day to record the number of normal seedling. d Determination of the seed density based on the observation of seed aging data. If data germination of seeds N0, N1, N2, N3, N4 is obtained, it will be known information density of seed to be used. Determination of seed density ratio is calculated based on multiples of sprouts that grow normally each lot N to control. Jelutung seed density using a seed as much as 50 grains with sprouts tube size 20 cm x 30 cm area = 600 cm2, the seed density d early or d0 = 50 grains 600 cm2. The density of the seed after seed aging in this study were Kartika, 1994: d1 = N1 N0 d  and the formula for the following seed density ....d2 = N2 N0 d  Bogor, 21-22 October 2015 820

2.4 Data analysis Experimental design was completely randomized design CRD.

Linear model experimental design: Yij=  + Ai + ij Where: Yijk = observations on the density level of the i-th  = average common Ai = the effect of treatment density to-i 3. RESULT AND DISCUSSION 3.1 Research results