134 135
dilaksanakan pada bulan Mei sampai denn Oktober 2012. Tiga faktor perlakuan yang dikombinasikan secara faktorial adalah dosis spora endomikoriza jenis Glomus sp 0; 25; 50 dan 75 butir; kompos
organik 0, 10 dan 20 gr dan pupuk pupuk N non organik 0 dan 5 gr, sehingga terdapat 24 kombinasi perlakuan. Perlakuan diuji dengan rancangan Acak Kelompok RAK dengan tiga ulangan.
Pemanfaatan pupuk hayati Glomus sp berdasarkan hasil penelitian sebelumnya Meti-unpublished data. Bibit tanaman mente diperoleh dari penanaman biji gelondongan mente yang diperoleh dari
petani mente di kawasan Kecamatan Kubu Kabupaten Karangasem.
Hasil analisa menunjukkan bahwa perlakuan kombinasi dosis spora endomikoriza 75 butir, pupuk organik 20 grdan pupuk N 0 gr memberikan pengaruh paling baik terhadap pertumbuhan bibit
mente jumlah daun dan tinggi bibit. Hasil ini penelitian juga menunjukkan bahwa penambahan pupuk hayati endomikriza tanpa pupuk organik dan N lebih baik dibandingkan dengan perlakuan tanpa
spora endomikoriza. Hasil penelitian ini menunjukkan bahwa penambahan pupuk hayati endomikroiza berpengaruh positif terhadap pertumbuhan bibit mente. Pupuk hayati endomikoriza berpengaruh secara
sinergis dengan pupuk organik namun tidak dengan pupuk non organikN.
1. Introduction
Vesicular Arbuscular Mycorrhizal VAM fungi are beneicial microorganisms that form symbiotic association with the ine roots of vascular plants and can be utilized as bio-fertilizers
supporting the plant growth because the mycorrhizal fungi play an important role in improving plant growth, nutrient uptake especially phosphorous Smith Read, 1997 ; Brundrett et al., 2008 and
provide stress tolerance and disease resistance to plants Smith et al., 2010. Efforts are being made to improve the quality of seedlings of forest trees under nursery conditions through inoculation
of suitable mycorrhizal strain alone or combined with other organic and an-organic fertilizer may improve the growth of seedlings Gill et al. 2002
Most host plants of non-arbuscular mycorrhizal fungi do not establish functional vesicular arbuscular mycorrhizal VAM symbiosis in nature Giovannetti and Sbrana 1998. Among the
known ectomycorrhizal hosts, Eucalyptus is an exception as it also forms vesicular arbuscular mycorrhiza Lapeyrie and Chilvers 1985. The present investigation analyses the effect of Glomus
sp spores number mixed with organic and an-organic fertilizer were observed on the growth of Anacardium occidentale L seedlings.
2. Materials And Methods
The spores of VAM fungi Glomus sp were isolated from the rhizosphere of Anacardium occidentale L plants and mass produced on maize Zea mays. Seedlings of Anacardium occidentale
L were procured from Divisional Forest Nursery of Bali Province.
Inoculation of Seedlings
One seedling of Anacardium occidentale L was grown in pots 30×30 cm in a sand soil mixture 1000:1000 g. Spores mycorrhizal Glomus sp i.e. 25, 50 and 75 of spores were added along
with infected roots. Observations were recorded after 15, 30, 45 and 60 days on seedlings shoot length increase in height, shoot weight and root weight. Percentage mycorrhizal root colonization,
mycorrhizal VAM spore number and phosphorous content of seedlings were studied. Three replicates of each treatment were taken.
Quantiication of VAM Spores, Root Colonization and Phosphorus Content
Percentage mycorrhizal root colonization was studied according to Kormanik dan Mc.Graw 1982 The VAM spore quantiication was also determined according to Gerdemann and Nicolson 1963.
Phosphorous content was determined by vanadomolybdate phosphoric yellow colour method Jackson 1958.
3. Results And Discussion
